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Volume 20, Issue 1
  • ISSN: 1573-4080
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Abstract

Background

The imbalance between free radical formation and antioxidant defence leads to the development of oxidative stress. The search for substances that would mitigate or prevent the effects of oxidative stress remains relevant.

Objective

Our goal was to compare the antioxidant and mitigation effects of L-glutamic acid (L-Glu) and N-acetylcysteine (NAC) alone or in combination using a battery of biomarkers of oxidative stress such as reduced glutathione (GSH) superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), glutathione reductase (GR), glutathione transferase (GST) and lipid peroxidation, determined as a content of lipid hydroperoxides (LOOH) and thiobarbituric acid reactive substances (TBARS). Histopathological examination of the liver was also performed.

Methods

Experimental rats were divided into five experimental groups. Exp.1: was treated with CCl only, Exp. 2: was treated with CCl/L-Glu, Exp. 3: was treated with CCl/Glu/NAC. Exp. 4: was treated with CCl/NAC, Control 5: served as the control rats.

Results

These findings suggest that the CCl leads to oxidative stress by depleting the antioxidant enzyme activities and increasing peroxidation products. The studied biochemical parameters were altered by the introduction of CCl, which was normalised (to one degree or another) by L-Glu, L-Glu/NAC and NAC treatment.

Conclusion

The most remarkable protective effect was observed in groups of rats that were treated with L-Glu only. This conclusion was confirmed by histopathological findings which showed less severe hepatocellular necrosis, fibrosis and inflammation in CCl/L- Glu and CCl/L-Glu/NAC treated group, compared to the CCl group.

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2024-03-01
2024-11-16

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/content/journals/cei/10.2174/0115734080257975230922050816
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Antioxidant and Hepatoprotective Effects of L-Glu and NAC against CCl4-induced Oxidative Damage in Rats. Biochemical and Histopathological Evaluation
Current Enzyme Inhibition 20, 40 (2024); https://doi.org/10.2174/0115734080257975230922050816
/content/journals/cei/10.2174/0115734080257975230922050816
/content/journals/cei/10.2174/0115734080257975230922050816
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  • Article Type:     Research Article
Keyword(s): antioxidant system; free radicals; L-Glu; NAC; Oxidative stress; xenobiotics

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