PCR: An Effective, Faster and Relevant Method for the Detection of Clinical Isolates of Candida albicans

Background: Candida albicans is associated with infectious diseases with colonization, in both immunocompromised and immunocompetent patients. Antibiotic treatment can develop candidiasis. Objective: Evaluation of ITS 4 and ITS5 primer expression on clinical isolates of C. albicans. Methods: Fifty clinical isolates of C. albicans were collected from different sites viz. Catheter Tip (CT), High Vaginal Swab (HVS) and Urine (U). Results: Using the universal fungal primers ITS4 and ITS5, PCR produced a single monomorphic amplification at 520 bp indicating that the strains are of same genetic identity. Conclusion: This is an effective, faster and relevant method for the detection and characterization of clinically isolated strains of C. albicans.