Skip to content
2000
  1. Home
  2. A-Z Publications
  3. Current Drug Metabolism
  4. Volume 25, Issue 8
  5. Article
Volume 25, Issue 8
  • ISSN: 1389-2002
  • E-ISSN: 1875-5453

Abstract

Background

(CF) is a herbal medicine with pharmacological activities such as antitumor, antiviral, antiallergic, antipruritic effects, and so on.

Objective

In this study, an ultra-high performance liquid chromatography/tandem mass spectrometry (UPLC-MS/MS) method was prepared and verified to measure the concentrations of seven analytes (bergapten, xanthotoxol, xanthotoxin, imperatorin, osthole, isopimpinellin, isoimperatorin) in HepG2 cells.

Methods

The separation of seven analytes was performed on an ACQUITY UPLC® BEH C18 column (2.1×100 mm, 1.7 μm) with a gradient mobile phase system of 0.1% formic acid/water and acetonitrile.

Results

The CV of analytes was within 7.77%, and the bias was in the range of -5.43%-3.84%. The matrix effects of analytes ranged from 92.95% to 104.58%, and the extraction recoveries ranged from 76.45% to 104.69%. The relative standard deviation of stability results was less than 8.21%, indicating that seven analytes were stable.

Conclusion

The method was successfully applied to the determination of the content of seven analytes of CF extracts by UPLC-MS/MS, and the results will provide a reference for the cellular pharmacokinetics of CF.

© 2024 Bentham Science Publishers
Loading

Article metrics loading...

/content/journals/cdm/10.2174/0113892002301262241107065717
2024-11-18
2025-04-13

  • From This Site

    /content/journals/cdm/10.2174/0113892002301262241107065717
    dcterms_title,dcterms_subject,pub_keyword
    -contentType:Contributor -contentType:Concept -contentType:Institution
    10
    5
Loading full text...

Full text loading...

References

  1. China Pharmacopoeia Committee Pharmacopoeia of the People’s Republic of China (The first Division).China Medical Science and Technology PressBeijing2020
     [Google Scholar]
  2. KhanS.A. WuY. LiA.S.M. FuX.Q. YuZ.L. Network pharmacology and molecular docking-based prediction of active compounds and mechanisms of action of Cnidii Fructus in treating atopic dermatitis.BMC Complement. Med. Ther.202222127510.1186/s12906‑022‑03734‑736261841
     [Google Scholar]
  3. ChenD. WangJ. JiangY. ZhouT. FanG. WuY. Separation and determination of coumarins in Fructus cnidii extracts by pressurized capillary electrochromatography using a packed column with a monolithic outlet frit.J. Pharm. Biomed. Anal.200950569570210.1016/j.jpba.2009.05.02619608371
     [Google Scholar]
  4. ChenZ. XuA. BiX. LuoW. LiJ. FanG. SunD. A fast and accurate method for the pharmacokinetic research of four coumarin analogs in Fructus cnidii using capillary electro-chromatography with a methacrylate ester-based monolithic column.Electrophoresis20173822-233036304710.1002/elps.20160042828714122
     [Google Scholar]
  5. SunY. YangA.W.H. LenonG.B. Phytochemistry, ethnopharmacology, pharmacokinetics and toxicology of Cnidium monnieri (L.) Cusson.Int. J. Mol. Sci.2020213100610.3390/ijms2103100632028721
     [Google Scholar]
  6. HuX. HuangW. YangY. Cytochrome P450 isoenzymes in rat and human liver microsomes associate with the metabolism of total coumarins in Fructus Cnidii.Eur. J. Drug Metab. Pharmacokinet.201540437337710.1007/s13318‑014‑0219‑424993184
     [Google Scholar]
  7. KordulewskaN.K. KostyraE. CieślińskaA. FiedorowiczE. JarmołowskaB. Cytokine production by PBMC and serum from allergic and non-allergic subjects following in vitro histamine stimulation to test fexofenadine and osthole anti-allergic properties.Eur. J. Pharmacol.201679176377210.1016/j.ejphar.2016.10.02027756601
     [Google Scholar]
  8. HussainM.I. SyedQ.A. KhattakM.N.K. HafezB. ReigosaM.J. El-KeblawyA. Natural product coumarins: Biological and pharmacological perspectives.Biologia (Bratisl.)201974786388810.2478/s11756‑019‑00242‑x
     [Google Scholar]
  9. JiangG. LiuJ. RenB. TangY. OwusuL. LiM. ZhangJ. LiuL. LiW. Anti-tumor effects of osthole on ovarian cancer cells in vitro.J. Ethnopharmacol.201619336837610.1016/j.jep.2016.08.04527566206
     [Google Scholar]
  10. LahiriD. DashS. DuttaR. NagM. Elucidating the effect of anti-biofilm activity of bioactive compounds extracted from plants.J. Biosci.20194425210.1007/s12038‑019‑9868‑431180065
     [Google Scholar]
  11. ZhangQ. QinL. HeW. Van PuyveldeL. MaesD. AdamsA. ZhengH. De KimpeN. Coumarins from Cnidium monnieri and their antiosteoporotic activity.Planta Med.2007731131910.1055/s‑2006‑95172417315308
     [Google Scholar]
  12. BasnetP. YasudaI. KumagaiN. TohdaC. NojimaH. KuraishiY. KomatsuK. Inhibition of itch-scratch response by fruits of Cnidium monnieri in mice.Biol. Pharm. Bull.20012491012101510.1248/bpb.24.101211558560
     [Google Scholar]
  13. LimE.G. KimG.T. LeeS.H. KimS.Y. KimY.M. Apoptotic effects of extract from Cnidium monnieri (L.) Cusson by adenosine monosphosphate-activated protein kinase-independent pathway in HCT116 colon cancer cells.Mol. Med. Rep.20161364681468810.3892/mmr.2016.511527082059
     [Google Scholar]
  14. YangL.L. WangM.C. ChenL.G. WangC.C. Cytotoxic activity of coumarins from the fruits of Cnidium monnieri on leukemia cell lines.Planta Med.200369121091109510.1055/s‑2003‑4518814750023
     [Google Scholar]
  15. SumiyoshiM. SakanakaM. TaniguchiM. BabaK. KimuraY. Anti-tumor effects of various furocoumarins isolated from the roots, seeds and fruits of Angelica and Cnidium species under ultraviolet A irradiation.J. Nat. Med.2014681839410.1007/s11418‑013‑0774‑z23649674
     [Google Scholar]
  16. FanY. ZhaoL. HuangX. ShenJ. WangW. JiaX. GaoM. OuyangH. ChangY. HeJ. UPLC-MS/MS method for the simultaneous quantification of eight compounds in rat plasma and its application to a pharmacokinetic study after oral administration of Veratrum (Veratrum nigrum L.) extractBiomed Res Int20202020888327710.1155/2020/8883277
     [Google Scholar]
  17. LvL. YouY. LiuY. YangZ. Advanced research in cellular pharmacokinetics and its cutting-edge technologies.Curr. Pharm. Des.202228373095310410.2174/138161282866622090710260636082865
     [Google Scholar]
  18. ZhangJ. ZhouF. WuX. ZhangX. ChenY. ZhaB.S. NiuF. LuM. HaoG. SunY. SunJ. PengY. WangG. Cellular pharmacokinetic mechanisms of adriamycin resistance and its modulation by 20(S)-ginsenoside Rh2 in MCF-7/Adr cells.Br. J. Pharmacol.2012165112013410.1111/j.1476‑5381.2011.01505.x21615726
     [Google Scholar]
  19. LiuJ. GengT. DuanK. GaoX. HuangC. WangJ. HuangW. HuangL. WangZ. XiaoW. Cellular pharmacokinetics and pharmacodynamics mechanisms of ginkgo diterpene lactone and its modulation of P-glycoprotein expression in human SH-SY5Y cells.Biomed. Chromatogr.20193312e469210.1002/bmc.469231452210
     [Google Scholar]
  20. TangC. NiuX. ShiL. ZhuH. LinG. XuR. In vivo pharmacokinetic drug-drug interaction studies between Fedratinib and antifungal agents based on a newly developed and validated UPLC/MS-MS method.Front. Pharmacol.20211162689710.3389/fphar.2020.62689733613287
     [Google Scholar]
  21. XuR. LinQ. QiuX. ChenJ. ShaoY. HuG. LinG. UPLC-MS/MS method for the simultaneous determination of imatinib, voriconazole and their metabolites concentrations in rat plasma.J. Pharm. Biomed. Anal.201916661210.1016/j.jpba.2018.12.03630594035
     [Google Scholar]
  22. ZhangY. LiuY. XieS. XuX. XuR. Evaluation of the inhibitory effect of quercetin on the pharmacokinetics of tucatinib in rats by a novel UPLC–MS/MS assay.Pharm. Biol.202260162162610.1080/13880209.2022.204886235289238
     [Google Scholar]
  23. LiJ. MaB. ZhangQ. YangX. SunJ. TangB. CuiG. YaoD. LiuL. GuG. ZhuJ. WeiP. OuyangP. Simultaneous determination of osthole, bergapten and isopimpinellin in rat plasma and tissues by liquid chromatography–tandem mass spectrometry.J. Chromatogr. B Analyt. Technol. Biomed. Life Sci.2014970778510.1016/j.jchromb.2014.06.01425240925
     [Google Scholar]
/content/journals/cdm/10.2174/0113892002301262241107065717
Loading
Application of UPLC-MS/MS to Study Cellular Pharmacokinetics of Seven Active Components of Cnidii Fructus Extracts
Current Drug Metabolism 25, 576 (2024); https://doi.org/10.2174/0113892002301262241107065717
/content/journals/cdm/10.2174/0113892002301262241107065717
/content/journals/cdm/10.2174/0113892002301262241107065717
Loading

Data & Media loading...

Supplements

Supplementary material is available on the publisher’s website along with the published article.

file format download Download

  • Article Type:     Research Article
Keyword(s): Cell; Cnidii Fructus; coumarin; herb medicine; pharmacokinetic; UPLC-MS/MS

Most Cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error
Please enter a valid_number test