Skip to content
2000
  1. Home
  2. A-Z Publications
  3. Current Drug Metabolism
  4. Fast Track Articles
  5. Fast Track Article
image of Application  of  UPLC-MS/MS  to  Study  Cellular  Pharmacokinetics of Seven Active Components of Cnidii Fructus Extracts

Abstract

Background

(CF) is a herbal medicine with pharmacological activities such as antitumor, antiviral, antiallergic, antipruritic effects, and so on.

Objective

In this study, an ultra-high performance liquid chromatography/tandem mass spectrometry (UPLC-MS/MS) method was prepared and verified to measure the concentrations of seven analytes (bergapten, xanthotoxol, xanthotoxin, imperatorin, osthole, isopimpinellin, isoimperatorin) in HepG2 cells.

Methods

The separation of seven analytes was performed on an ACQUITY UPLC® BEH C18 column (2.1×100 mm, 1.7 μm) with a gradient mobile phase system of 0.1% formic acid/water and acetonitrile.

Results

The CV of analytes was within 7.77%, and the bias was in the range of -5.43%-3.84%. The matrix effects of analytes ranged from 92.95% to 104.58%, and the extraction recoveries ranged from 76.45% to 104.69%. The relative standard deviation of stability results was less than 8.21%, indicating that seven analytes were stable.

Conclusion

The method was successfully applied to the determination of the content of seven analytes of CF extracts by UPLC-MS/MS, and the results will provide a reference for the cellular pharmacokinetics of CF.

© 2024 Bentham Science Publishers
Loading

Article metrics loading...

/content/journals/cdm/10.2174/0113892002301262241107065717
2024-11-18
2025-01-24

  • From This Site

    /content/journals/cdm/10.2174/0113892002301262241107065717
    dcterms_title,dcterms_subject,pub_keyword
    -contentType:Contributor -contentType:Concept -contentType:Institution
    10
    5
Loading full text...

Full text loading...

References

  1. China Pharmacopoeia Committee Pharmacopoeia of the People’s Republic of China (The first Division). China Medical Science and Technology Press Beijing 2020
    [Google Scholar]
  2. Khan S.A. Wu Y. Li A.S.M. Fu X.Q. Yu Z.L. Network pharmacology and molecular docking-based prediction of active compounds and mechanisms of action of Cnidii Fructus in treating atopic dermatitis. BMC Complement. Med. Ther. 2022 22 1 275 10.1186/s12906‑022‑03734‑7 36261841
    [Google Scholar]
  3. Chen D. Wang J. Jiang Y. Zhou T. Fan G. Wu Y. Separation and determination of coumarins in Fructus cnidii extracts by pressurized capillary electrochromatography using a packed column with a monolithic outlet frit. J. Pharm. Biomed. Anal. 2009 50 5 695 702 10.1016/j.jpba.2009.05.026 19608371
    [Google Scholar]
  4. Chen Z. Xu A. Bi X. Luo W. Li J. Fan G. Sun D. A fast and accurate method for the pharmacokinetic research of four coumarin analogs in Fructus cnidii using capillary electro‐chromatography with a methacrylate ester‐based monolithic column. Electrophoresis 2017 38 22-23 3036 3047 10.1002/elps.201600428 28714122
    [Google Scholar]
  5. Sun Y. Yang A.W.H. Lenon G.B. Phytochemistry, ethnopharmacology, pharmacokinetics and toxicology of Cnidium monnieri (L.) Cusson. Int. J. Mol. Sci. 2020 21 3 1006 10.3390/ijms21031006 32028721
    [Google Scholar]
  6. Hu X. Huang W. Yang Y. Cytochrome P450 isoenzymes in rat and human liver microsomes associate with the metabolism of total coumarins in Fructus Cnidii. Eur. J. Drug Metab. Pharmacokinet. 2015 40 4 373 377 10.1007/s13318‑014‑0219‑4 24993184
    [Google Scholar]
  7. Kordulewska N.K. Kostyra E. Cieślińska A. Fiedorowicz E. Jarmołowska B. Cytokine production by PBMC and serum from allergic and non-allergic subjects following in vitro histamine stimulation to test fexofenadine and osthole anti-allergic properties. Eur. J. Pharmacol. 2016 791 763 772 10.1016/j.ejphar.2016.10.020 27756601
    [Google Scholar]
  8. Hussain M.I. Syed Q.A. Khattak M.N.K. Hafez B. Reigosa M.J. El-Keblawy A. Natural product coumarins: Biological and pharmacological perspectives. Biologia (Bratisl.) 2019 74 7 863 888 10.2478/s11756‑019‑00242‑x
    [Google Scholar]
  9. Jiang G. Liu J. Ren B. Tang Y. Owusu L. Li M. Zhang J. Liu L. Li W. Anti-tumor effects of osthole on ovarian cancer cells in vitro. J. Ethnopharmacol. 2016 193 368 376 10.1016/j.jep.2016.08.045 27566206
    [Google Scholar]
  10. Lahiri D. Dash S. Dutta R. Nag M. Elucidating the effect of anti-biofilm activity of bioactive compounds extracted from plants. J. Biosci. 2019 44 2 52 10.1007/s12038‑019‑9868‑4 31180065
    [Google Scholar]
  11. Zhang Q. Qin L. He W. Van Puyvelde L. Maes D. Adams A. Zheng H. De Kimpe N. Coumarins from Cnidium monnieri and their antiosteoporotic activity. Planta Med. 2007 73 1 13 19 10.1055/s‑2006‑951724 17315308
    [Google Scholar]
  12. Basnet P. Yasuda I. Kumagai N. Tohda C. Nojima H. Kuraishi Y. Komatsu K. Inhibition of itch-scratch response by fruits of Cnidium monnieri in mice. Biol. Pharm. Bull. 2001 24 9 1012 1015 10.1248/bpb.24.1012 11558560
    [Google Scholar]
  13. Lim E.G. Kim G.T. Lee S.H. Kim S.Y. Kim Y.M. Apoptotic effects of extract from Cnidium monnieri (L.) Cusson by adenosine monosphosphate-activated protein kinase-independent pathway in HCT116 colon cancer cells. Mol. Med. Rep. 2016 13 6 4681 4688 10.3892/mmr.2016.5115 27082059
    [Google Scholar]
  14. Yang L.L. Wang M.C. Chen L.G. Wang C.C. Cytotoxic activity of coumarins from the fruits of Cnidium monnieri on leukemia cell lines. Planta Med. 2003 69 12 1091 1095 10.1055/s‑2003‑45188 14750023
    [Google Scholar]
  15. Sumiyoshi M. Sakanaka M. Taniguchi M. Baba K. Kimura Y. Anti-tumor effects of various furocoumarins isolated from the roots, seeds and fruits of Angelica and Cnidium species under ultraviolet A irradiation. J. Nat. Med. 2014 68 1 83 94 10.1007/s11418‑013‑0774‑z 23649674
    [Google Scholar]
  16. Fan Y. Zhao L. Huang X. Shen J. Wang W. Jia X. Gao M. Ouyang H. Chang Y. He J. UPLC-MS/MS method for the simultaneous quantification of eight compounds in rat plasma and its application to a pharmacokinetic study after oral administration of Veratrum (Veratrum nigrum L.) extract Biomed Res Int 2020 2020 8883277 10.1155/2020/8883277
    [Google Scholar]
  17. Lv L. You Y. Liu Y. Yang Z. Advanced research in cellular pharmacokinetics and its cutting-edge technologies. Curr. Pharm. Des. 2022 28 37 3095 3104 10.2174/1381612828666220907102606 36082865
    [Google Scholar]
  18. Zhang J. Zhou F. Wu X. Zhang X. Chen Y. Zha B.S. Niu F. Lu M. Hao G. Sun Y. Sun J. Peng Y. Wang G. Cellular pharmacokinetic mechanisms of adriamycin resistance and its modulation by 20(S)‐ginsenoside Rh2 in MCF‐7/Adr cells. Br. J. Pharmacol. 2012 165 1 120 134 10.1111/j.1476‑5381.2011.01505.x 21615726
    [Google Scholar]
  19. Liu J. Geng T. Duan K. Gao X. Huang C. Wang J. Huang W. Huang L. Wang Z. Xiao W. Cellular pharmacokinetics and pharmacodynamics mechanisms of ginkgo diterpene lactone and its modulation of P‐glycoprotein expression in human SH‐SY5Y cells. Biomed. Chromatogr. 2019 33 12 e4692 10.1002/bmc.4692 31452210
    [Google Scholar]
  20. Tang C. Niu X. Shi L. Zhu H. Lin G. Xu R. In vivo pharmacokinetic drug-drug interaction studies between Fedratinib and antifungal agents based on a newly developed and validated UPLC/MS-MS method. Front. Pharmacol. 2021 11 626897 10.3389/fphar.2020.626897 33613287
    [Google Scholar]
  21. Xu R. Lin Q. Qiu X. Chen J. Shao Y. Hu G. Lin G. UPLC-MS/MS method for the simultaneous determination of imatinib, voriconazole and their metabolites concentrations in rat plasma. J. Pharm. Biomed. Anal. 2019 166 6 12 10.1016/j.jpba.2018.12.036 30594035
    [Google Scholar]
  22. Zhang Y. Liu Y. Xie S. Xu X. Xu R. Evaluation of the inhibitory effect of quercetin on the pharmacokinetics of tucatinib in rats by a novel UPLC–MS/MS assay. Pharm. Biol. 2022 60 1 621 626 10.1080/13880209.2022.2048862 35289238
    [Google Scholar]
  23. Li J. Ma B. Zhang Q. Yang X. Sun J. Tang B. Cui G. Yao D. Liu L. Gu G. Zhu J. Wei P. Ouyang P. Simultaneous determination of osthole, bergapten and isopimpinellin in rat plasma and tissues by liquid chromatography–tandem mass spectrometry. J. Chromatogr. B Analyt. Technol. Biomed. Life Sci. 2014 970 77 85 10.1016/j.jchromb.2014.06.014 25240925
    [Google Scholar]
/content/journals/cdm/10.2174/0113892002301262241107065717
Loading
Application  of  UPLC-MS/MS  to  Study  Cellular  Pharmacokinetics of Seven Active Components of Cnidii Fructus Extracts
Bentham Science Publishers ; https://doi.org/10.2174/0113892002301262241107065717
/content/journals/cdm/10.2174/0113892002301262241107065717
/content/journals/cdm/10.2174/0113892002301262241107065717
Loading

Data & Media loading...

Supplements

Supplementary material is available on the publisher's website along with the published article.

file format download Download

  • Article Type:     Research Article
Keywords: coumarin ; UPLC-MS/MS ; pharmacokinetic ; herb medicine ; Cnidii fructus ; Cell

Most Cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error
Please enter a valid_number test