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image of Evaluation of Anti-Proliferative and Apoptosis-Inducing Activities of the New Ciprofloxacin Derivative on Human Leukemia NB4 Cells

Abstract

Backgrounds

Leukemic stem cells are considered to be the main cause of treatment failure and disease recurrence due to their resistance to most common therapies. Apoptosis induction is one of the highly effective methods for treating cancer. Ciprofloxacin is among the compounds whose antitumor effects have been confirmed.

Objectives

In this study, we investigated the anti-proliferative effect and induction of apoptosis by one of the derivatives of this family called (ICH-CP) on NB4 cell line as an model of Acute promyelocytic leukemia (APL). NB4 cells were treated using the ICH-CP combination in various concentrations.

Methods

The viability of NB4 cells was evaluated by MTT assay, and their morphology of apoptosis was examined by fluorescence microscopy. Flow cytometry and Annexin V/PI staining were used to quantify apoptosis. Finally, the expression of three genes, Bax, Bcl-2, and Survivin was inquired by real-time PCR.

Results

According to the results, ICH-CP was able to destroy about 60% of NB4 cells in a dose and time-dependent manner. Light microscopy and fluorescence microscopy studies on treated cells confirmed the induction of apoptosis. Also, the real-time PCR analysis showed that ICH-CP induces apoptosis in the NB4 cell line the down-regulation of Survivin and Bcl-2, in contrast to the up-regulation of the Bax gene.

Conclusion

Based on the present data, it seems that the novel compound can be a good candidate for the treatment of acute myeloid leukemia. Furthermore, it is recommended to evaluate the qualification of ICH-CP as an adjunctive agent for other cancer cell lines.

© 2024 Bentham Science Publishers
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2024-10-11
2024-11-22

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/content/journals/ccb/10.2174/0122127968280272240925103621
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Evaluation of Anti-Proliferative and Apoptosis-Inducing Activities of the New Ciprofloxacin Derivative on Human Leukemia NB4 Cells
Bentham Science Publishers ; https://doi.org/10.2174/0122127968280272240925103621
/content/journals/ccb/10.2174/0122127968280272240925103621
/content/journals/ccb/10.2174/0122127968280272240925103621
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  • Article Type:     Research Article
Keywords: survivin ; NB4 cells ; cell cycle ; Apoptosis ; Bcl-2

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