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Volume 21, Issue 2
  • ISSN: 1573-4072
  • E-ISSN: 1875-6646

Abstract

Background

Marine algae stand out as repositories of bioactive metabolites, widely harnessed in treating diverse diseases. Given the often-challenging side effects associated with conventional cancer treatments, researchers are increasingly turning their attention to marine algae as a promising source for discovering novel and potent anticancer compounds. In this study, we meticulously explored the potential anticancer activity of and sourced from the Indian Ocean.

Materials and Methods

The secondary metabolites were extracted using methanol solvent according to the standard protocol. The extract was subjected to GC-MS analysis and evaluated for its short- and long-term cytotoxicity and anti-proliferative ability in the HCT116 cell line using MTT, clonogenic, and trypan blue dye exclusion assays, respectively.

Results and Discussion

The results indicate that increasing the concentration of algal extracts decreased the cell viability significantly, with an IC of 51.49 µg/mL () and 37.50 µg /mL () in the HCT116 cell line. The results showed a significant reduction in colorectal cancer colony formation and a gradual reduction in cell proliferation. These results underscore the potential for targeted, low-toxicity biomedicines due to their selective cytotoxicity against cancer cells.

Conclusion

Our findings show that & extracts contain various bioactive compounds, display long-term and short-term cytotoxicity and anti-proliferative effects, which indicates that their constituent compounds might be further refined into effective anti-colorectal cancer medications.

© 2025 Bentham Science Publishers
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2024-04-24
2025-06-24

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Anticancer Activity and GC-MS Profiling of Bioactive Constituents in the Methanolic Extracts of Spatoglossum variabile and Gracilaria corticata
Curr. Bioact. Compd. 21, E240424229273 (2025); https://doi.org/10.2174/0115734072296835240409124751
/content/journals/cbc/10.2174/0115734072296835240409124751
/content/journals/cbc/10.2174/0115734072296835240409124751
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  • Article Type:     Research Article
Keyword(s): bioactive compounds; cytotoxicity assay; GC-MS analysis; HCT116; in-vitro; Marine algae

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