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Volume 21, Issue 2
  • ISSN: 1573-4072
  • E-ISSN: 1875-6646

Abstract

Background

In the past few decades, researchers have focused on finding the benefits of natural substances derived from plants.

Objective

This study aimed to evaluate the use of Brassica rapa seeds in poultry feed as an antioxidant and immunostimulant of host defenses.

Methods

We prepared three extracts using ethanol, Ethyl Acetate, and water. Spectrophotometric methods determined the total phenolic and flavonoid content in the three extracts. Antioxidant activity was assessed using 1,1-diphenyl-2-picrylhydrazyl (DPPH), ferric reducing/antioxidant power (FRAP), 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS) radicals and β-Carotene bleaching methods. An assessment of their immunostimulant activities was performed on chicken immunity cell proliferation (splenocytes, thymocytes, and Bursa cells) and IgY production.

Results

The total phenolic contents ranged from 462.5 to 794.8 mg /g of extract, the order of TPC was as follows: EtOAc > EtOH >Water. Flavonoid contents were, 63.7 mg in EtOH extract, 81.2 mg in EtOAc extract, and 108.7 mg/g Extract in aqueous extract. By DPPH, the IC of EtOH, EtOAc, and water, were 1.8 µg/ml, 2.4 µg/ml, and 1.5 µg/ml, respectively. Using the Ferric-Reducing Antioxidant Power (FRAP), we remarked that the EtOH and EtOAc extracts have important antioxidant powers. The ABTS assay indicated that EtOH and water had the highest activities with IC of 0.19 and 0.07, respectively. Finally, by the β-carotene bleaching test, we observed that the IC of the EtOH, EtOAc, and water were 62.1 µg/mL; 72.7 µg/mL, and 45.8 µg/mL, respectively. The results obtained indicate that Brassica aqueous extract stimulates humoral immunity by stimulating splenocyte (B and T-lymphocytes) and Bursa cell (B-lymphocyte) proliferation by more than 200% of response control. In addition, the aqueous extract highly stimulated the function of bursa cells by 208% of the reaction. In the same conditions, we recorded a stimulation of cellular immunity mediated by thymocytes by an increase in cell proliferation (352.7% of response) implicated in virus protection. These extracts also possessed an antimicrobial effect against diverse microorganisms such as coliforms and . The FT-IR spectrum indicated that the hydroxyl group (phenol), hydroxybenzoic acid family, carbohydrate molecules such as glucopyranose, a carbonyl ester group, hydrocarbon chains (alkyls groups) were present.

Conclusion

This result could have interesting applications in the poultry feed industry to enhance the performance of animal development.

© 2025 Bentham Science Publishers
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2024-04-09
2025-06-19

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/content/journals/cbc/10.2174/0115734072282302240402043805
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Antioxidant Properties of Brassica Rapa L. Seeds and Immunostimulant Effect on Immunity Broilers Cells (Gallus Gallus Domesticus)
Curr. Bioact. Compd. 21, E090424228866 (2025); https://doi.org/10.2174/0115734072282302240402043805
/content/journals/cbc/10.2174/0115734072282302240402043805
/content/journals/cbc/10.2174/0115734072282302240402043805
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  • Article Type:     Research Article
Keyword(s): antioxidants; Brassica rapa seeds; chicken immunity cells; FT-IR; immunostimulation; poultry feed

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