Current Analytical Chemistry - Volume 7, Issue 1, 2011

This special issue is aimed at providing a recent update on the analytical mainly electroanalytical techniques used in the development of electrochemical DNA biosensors regarding DNA structure and DNA hybridization. It also addresses the significance of electroanalytical techniques as an alternative and even more as pioneering analytical techniques in the study of epigenetic DNA modification such as methylation. Th Read More

Process and quality control requires fast and reliable obtaining of results in all topics of chemical analysis. Flow-through analytical systems offer unique features and advantages, particularly regarding a possibility of automation of key steps of measurement in analytical chemistry. Principles of flow injection analysis (FIA) have been numerously utilized in connection with the detection by biosensors and bioassay. While the flow Read More

The development of DNA-sensors has become significantly important in the past decades due to prospects of application in medicine, biotechnology and exploring fundamental problems related to cell biology and DNA functioning. Layer-by-layer (LbL) immobilization provides unique approach to the implementation of DNA into the surface sensing layers, a crucial step of DNA-sensor development. The review consider Read More

Osmium tetroxide complexes with nitrogenous ligands and analogous complexes of six-valent osmium proved excellent tools for selective labeling of biopolymers (nucleic acids, proteins and polysaccharides). Reactions of these species with target moieties within the biopolymer molecules (pyrimidine nucleobases, tryptophan residues or sugar moieties) are facile at physiological conditions and are in general structure-select Read More

Electrochemical redox compounds, which can interact or bind with different affinities to single and double stranded DNA and thus are referred to as electrochemical hybridization indicators, represent a powerful and, along with this, simple tool for electrochemical genetic analysis. In this paper, general tendencies and recent advances in this field, including the modes of DNA-redox indicators interactions, approaches for their ele Read More

Electrochemical biosensors have a key importance in many areas including clinical, biological, pharmaceutical, forensic, environmental and agricultural applications. They have the advantages of rapid, easy and low cost preparation allowing the localization of biocompound on electrodes of any size with independence of sample turbidity, high sensitivity and high selectivity. Electrochemical devices have attracted considera Read More

The application of electrochemical measurements of the redox couples of metal-based antitumor potential drugs in the presence of DNA is a highly sensitive method due to the resemblance between the electrochemical and biological reactions. CV provides a useful complement to the other biophysical methods of investigations such as UV-visible spectroscopy and fluorescence studies. Since the redox active metal complexes Read More

A great progress in the development of electrochemical carbon paste sensors for DNA hybridization and DNA damage achieved in recent years suggests that these sensors may soon become important tools in medicine and other areas of practical life of the 21st century. The article illustrates a growing number of applications of carbon electrodes in analytical chemistry. Various modifications of carbon pastes and car Read More