Current Analytical Chemistry - Volume 7, Issue 3, 2011

A Phospholipase A2 termed as BSS PLA2 was purified from the venom of Bungarus sindanus sindanus (Sindhi Krait) through two steps: size exclusion chromatography on sephadex G-75 and reversed-phase chromatography on Vydac C18 HPLC column. The homogeneity and molecular mass of BSS PLA2 were analysed by SDS-PAGE and MALDI-TOF mass spectrometry. The N-terminal sequence of 32 amino acids of the B Read More

Duloxetine (DLX) is a selective orally administered serotonin and norepinephrine reuptake inhibitor usually used in the treatment of major depressive disorder. 1-Naphthol (NPH) is a toxic impurity produced when DLX is exposed to acid medium and it also proceeds from the synthesis material. A simple and rapid stability indicating capillary micellar electrokinetic chromatography method was developed and validated fo Read More

Interaction between prulifloxacin (PUFX) and pepsin was studied under a simulated physiological condition using fluorescence spectrophotometric techniques when the Eu(III) exists. The fluorescence emission intensity of pepsin was strongly quenched by the addition of prulifloxacin. Spectrophotometric observations are rationalized in terms of a static quenching process. The binding constants and the number of bindi Read More

Two nicotine (NC)-selective electrode systems were developed with different techniques. Precipitation based technique with tungstophosphoric acid as cationic exchanger in poly (vinyl chloride) (PVC) matrix was used for sensor 1 fabrication. 2,9,16,23-tetra-tert-butylphethalocynine (TTBPC) based technique with and without potassium tetrakis[3,5- bis(trifluoromethyl)phenyl]borate (KTFPB), as fixed anionic site in poly (vinyl ch Read More

The kinetic study of the thermal oxidative rancidification of extra-virgin olive oils at 98 °C carried out according to the A.O.M. (American Oxygen Method - official method) of the A.O.C.S. (American Oil Chemistry Society) was completed. The orders of magnitude for the values of the kinetic rate constant k (10-4 min-1) for this process, the activation energy Ea (38 kJ mol-1) and the reaction order n (1st order) were definitively Read More

A new high-performance thin-layer chromatographic (HPTLC) method has been established for determination of mycophenolate mofetil in human plasma. Mycophenolate mofetil is used as an important immunosuppressive agent. Chromatographic separation was performed on aluminium plates coated with silica gel 60F254; the mobile phase was a combination of triethylamine buffer (pH 5.3) and acetonitrile in the ratio of Read More

A method has been developed for the direct determination of Cu, Cd, Ni and Pb in aquatic humic substances (AHS) by graphite furnace atomic absorption spectrometry. AHS were isolated from water samples rich in organic matter, collected in the Brazilian Ecological Parks. All analytical curves presented good linear correlation coefficient. The limits of detection and quantification were in the ranges 2.5-16.7 μg g-1 and 8. Read More

Hybrid sorbent based on nano alumina - single walled carbon nanotube was applied to preconcentration of Pb(II), Cu(II) and Co(II) from various samples via solid phase extraction technique. The determination of Pb(II), Co(II) and Cu(II) ions was made by flame atomic absorption spectrometry (FAAS). Experimental parameters including pH of sample solution, sample volume, flow rate of sample solution, volume and concentrati Read More

This work described the electrochemical response characteristic of poly (vinyl chloride) (PVC) membrane sensor and coated wire ion selective electrode (CWISE) based on the use of copper (II) salicylidene-N- anilinoacetohydrazone ionophore. o-nitrophenyloctylether (o-NPOE) is used as a plasticizer in the PVC matrix. The membrane sensors were prepared and the performance characteristics were evaluated according to I Read More

Metallothioneins (MTs) are a group of low molecular mass, cysteine-rich proteins with a variety of functions including involvement in metal homeostasis, free radical scavenging, protection against heavy metal damage, and metabolic regulation via Zn donation. The overexpression of MTs in proliferating cells is turning the attention to the study of MTs as novel promising marker of tumor diseases. Besides, levels of MTs i Read More

A novel ionic liquid-based microextraction methodology was developed for preconcentration of ultra-traces of arsenic prior to its determination by electrothermal atomic absorption spectrometry (ETAAS). As(III) was extracted as ammonium pyrroldinedithiocarbamate (APDC) complex from 10 mL sample into a 50 μL-volume of ionic liquid, 1- hexyl-3-methylimidazolium hexafluorophosphate, with the aid of sonication in an ultras Read More