Current Analytical Chemistry - Volume 2, Issue 3, 2006

The possibility of determining the content of various active pharmaceutical principles (Carbamazepine, Diclofenac, Ofloxacin, Sulfamethoxazol, Clofibric acid and Propranolol) in water and in commercial formulation by Voltammetric- Polarographic methods has been investigated. The considered pharmaceutical principles are the ones selected by the Rempharmawater European Union Program, as belonging to the clas Read More

Several alternative methods of conducting affinity electrophoresis are described, including countercurrent affinity electrophoresis, antibody affinity electrophoresis, functional affinity electrophoresis, and avidin functional affinity electrophoresis. The basis of these new techniques is to catch the prey molecules while they migrate electrophoretically through the immobilized bait molecules region of the polyacrylamide gel. Potentia Read More

A new flow injection method for the determination of levofloxacin based on the enhancement of the intensity of chemiluminescence (CL) from the luminol-KIO4 system was described in this paper. The enhanced chemiluminescence intensity was linear with the concentration of levofloxacin over the range from 7 ng mL-1 to 1000 ng mL-1 (r2ε0.9991) with a detection limit (3s) of 2 ng mL-1. At a flow rate of 2.0 mL min-1, a comple Read More

The study of telomeric DNA has acquired importance through the discovery of the relationship between telomerase activity in most cancer cells and telomere folding into tetraplex structure (guanine-quadruplex). G-quadruplexes exhibit four-stranded structure containing one or more nucleic acid strands with a central channel able to accommodate metal cations. Coordination of certain metal cations stabilizes G-quadruplex as Read More

This paper reviews the use of X-ray fluorescence spectrometry (XRFS) for non-destructive studies of valuable metallic objects in the fields of jewelry, archaeology and art, as well as its application in the analysis of coatings and solutions, due to the interest of these issues in jewelry manufacture. The general features of the technique as well as the most outstanding results obtained in the commented fields are descri Read More

The layer-by-layer assembly technique is a rich, versatile, and significantly inexpensive approach to the formation of thin films via alternating adsorption of positively and negatively charged species from aqueous solutions. Polymer organic and organic/inorganic thin films formed using this technique may contain a number of different functional groups, including electro-optic, electrochromics, conducting, dielectric layers wit Read More

The analytical community continues to search for cost-effective, reliable, and even portable analytical techniques that can give reliable and fast-response results for a variety of biochemicals. Advances in the field of microelectro- mechanical systems (MEMS) and their uses now offer unique opportunities in the design of ultrasensitive analytical devices. Microcantilevers (MCLs) have been emerged as a novel unique platform f Read More

The methylation of DNA is an epigenetic modification that can play an important role in the control of gene expression in mammalian cells. The aberrant or accidental methylation of CpG islands in the promoter region has been observed for many cancer-related genes resulting in the silencing of their expression. Hence, it has become a focus of scientific attraction currently. Many of the early procedures developed gav Read More

DNA mutation is the major cause of gene evolution. The variation of DNA sequence may result in genetic diseases, genotypes, single nucleotide polymorphism, and drug resistance of pathogens. Using biochip technologies, detection of mutations can be performed in parallel under identical conditions, hereby providing a solution to continuously increasing demands for large-scale genetic analysis. This article reviews the deve Read More

The mathematical models are established for conventional atomic spectrometric signals by simulating experimental results with computer. The laboratory-made derivative measurement system is described. The mathematical models of derivative signals are developed for flame atomic absorption spectrometry (FAAS), cold vapour atomic absorption spectrometry (CVAAS), hydride generation atomic absorption spectrom Read More