Rapid Determination of Levofloxacin at Nanogram Level in Pharmaceuticals and Biological Fluids Using Flow Injection Chemiluminescence

A new flow injection method for the determination of levofloxacin based on the enhancement of the intensity of chemiluminescence (CL) from the luminol-KIO4 system was described in this paper. The enhanced chemiluminescence intensity was linear with the concentration of levofloxacin over the range from 7 ng mL-1 to 1000 ng mL-1 (r2ε0.9991) with a detection limit (3s) of 2 ng mL-1. At a flow rate of 2.0 mL min-1, a complete analytical process could be performed within 0.5 minute, with a relative standard deviation of less than 3.0% (nε5). The recovery for the levofloxacin samples was from 91.0% to 109.4%. The proposed method was applied successfully in the determination of levofloxacin in tablets, eyedrop, human serum and urine samples.