Skip to content
2000
image of Analysis of Oral and Gut Microbiota Composition in Children with Dental Caries by NGS Approaches

Abstract

Objectives

This study aimed to characterize oral and gut microbiota of children with high dmft index and caries-free children at phylum, family and species levels as well as to evaluate the effect of M18 DSM 14685 (Carioblis) administration on microbiota composition of caries active children.

Materials and Methods

Ten children with active caries and nine caries-free children have been recruited. Four samples from different oral niches and stools were collected from each patient for the NGS sequencing of 16s Microbiota rDNA by S5 Ion Torrent.

Results

Our results revealed modifications in the microbiota composition of teeth, saliva and vestibular regions of the oral cavity and faecal samples in the presence of dental caries. These changes were evident at the family and species levels, with no significant differences found at the phylum composition level. In particular, Streptococcaceae were positively correlated to the high degree of caries in all niches, and the analysis at the species level led to the identification of 39 bacterial species significantly modulated in the analyzed groups. The use of probiotic seemed to exert beneficial effects on oral but not on faeces dysbiosis. The intestinal tract was confirmed to have a different microbiota composition compared to the oral cavity.

Conclusions

Dental caries mainly lead to modifications in the oral microbiota composition. DSM 14685 administration determines a shift in the oral microbiota composition towards a healthier state. Concerning the gastrointestinal tract, our study found for the first time that caries cause the increase of two bacterial species, related to other disorders: and .

© 2024 Bentham Science Publishers
Loading

Article metrics loading...

/content/journals/raiad/10.2174/0127722708335159241117062704
2024-12-04
2025-07-28

  • From This Site

    /content/journals/raiad/10.2174/0127722708335159241117062704
    dcterms_title,dcterms_subject,pub_keyword
    -contentType:Contributor -contentType:Concept -contentType:Institution
    10
    5
Loading full text...

Full text loading...

References

  1. Guideline on management of the developing dentition and occlusion in pediatric dentistry. Pediatr. Dent. 1990 27 143 155
    [Google Scholar]
  2. Wang Y. Wang S. Wu C. Chen X. Duan Z. Xu Q. Jiang W. Xu L. Wang T. Su L. Wang Y. Chen Y. Zhang J. Huang Y. Tong S. Zhou C. Deng S. Qin N. Oral microbiome alterations associated with early childhood caries highlight the importance of carbohydrate metabolic activities. mSystems 2019 4 6 00450-19 10.1128/msystems.00450‑19 31690590
    [Google Scholar]
  3. James S.L. Abate D. Abate K.H. Abay S.M. Abbafati C. Abbasi N. Abbastabar H. Abd-Allah F. Abdela J. Abdelalim A. Abdollahpour I. Abdulkader R.S. Abebe Z. Abera S.F. Abil O.Z. Abraha H.N. Abu-Raddad L.J. Abu-Rmeileh N.M.E. Accrombessi M.M.K. Acharya D. Acharya P. Ackerman I.N. Adamu A.A. Adebayo O.M. Adekanmbi V. Adetokunboh O.O. Adib M.G. Adsuar J.C. Afanvi K.A. Afarideh M. Afshin A. Agarwal G. Agesa K.M. Aggarwal R. Aghayan S.A. Agrawal S. Ahmadi A. Ahmadi M. Ahmadieh H. Ahmed M.B. Aichour A.N. Aichour I. Aichour M.T.E. Akinyemiju T. Akseer N. Al-Aly Z. Al-Eyadhy A. Al-Mekhlafi H.M. Al-Raddadi R.M. Alahdab F. Alam K. Alam T. Alashi A. Alavian S.M. Alene K.A. Alijanzadeh M. Alizadeh-Navaei R. Aljunid S.M. Alkerwi A. Alla F. Allebeck P. Alouani M.M.L. Altirkawi K. Alvis-Guzman N. Amare A.T. Aminde L.N. Ammar W. Amoako Y.A. Anber N.H. Andrei C.L. Androudi S. Animut M.D. Anjomshoa M. Ansha M.G. Antonio C.A.T. Anwari P. Arabloo J. Arauz A. Aremu O. Ariani F. Armoon B. Ärnlöv J. Arora A. Artaman A. Aryal K.K. Asayesh H. Asghar R.J. Ataro Z. Atre S.R. Ausloos M. Avila-Burgos L. Avokpaho E.F.G.A. Awasthi A. Ayala Quintanilla B.P. Ayer R. Azzopardi P.S. Babazadeh A. Badali H. Badawi A. Bali A.G. Ballesteros K.E. Ballew S.H. Banach M. Banoub J.A.M. Banstola A. Barac A. Barboza M.A. Barker-Collo S.L. Bärnighausen T.W. Barrero L.H. Baune B.T. Bazargan-Hejazi S. Bedi N. Beghi E. Behzadifar M. Behzadifar M. Béjot Y. Belachew A.B. Belay Y.A. Bell M.L. Bello A.K. Bensenor I.M. Bernabe E. Bernstein R.S. Beuran M. Beyranvand T. Bhala N. Bhattarai S. Bhaumik S. Bhutta Z.A. Biadgo B. Bijani A. Bikbov B. Bilano V. Bililign N. Bin Sayeed M.S. Bisanzio D. Blacker B.F. Blyth F.M. Bou-Orm I.R. Boufous S. Bourne R. Brady O.J. Brainin M. Brant L.C. Brazinova A. Breitborde N.J.K. Brenner H. Briant P.S. Briggs A.M. Briko A.N. Britton G. Brugha T. Buchbinder R. Busse R. Butt Z.A. Cahuana-Hurtado L. Cano J. Cárdenas R. Carrero J.J. Carter A. Carvalho F. Castañeda-Orjuela C.A. Castillo Rivas J. Castro F. Catalá-López F. Cercy K.M. Cerin E. Chaiah Y. Chang A.R. Chang H-Y. Chang J-C. Charlson F.J. Chattopadhyay A. Chattu V.K. Chaturvedi P. Chiang P.P-C. Chin K.L. Chitheer A. Choi J-Y.J. Chowdhury R. Christensen H. Christopher D.J. Cicuttini F.M. Ciobanu L.G. Cirillo M. Claro R.M. Collado-Mateo D. Cooper C. Coresh J. Cortesi P.A. Cortinovis M. Costa M. Cousin E. Criqui M.H. Cromwell E.A. Cross M. Crump J.A. Dadi A.F. Dandona L. Dandona R. Dargan P.I. Daryani A. Das Gupta R. Das Neves J. Dasa T.T. Davey G. Davis A.C. Davitoiu D.V. De Courten B. De La Hoz F.P. De Leo D. De Neve J-W. Degefa M.G. Degenhardt L. Deiparine S. Dellavalle R.P. Demoz G.T. Deribe K. Dervenis N. Des Jarlais D.C. Dessie G.A. Dey S. Dharmaratne S.D. Dinberu M.T. Dirac M.A. Djalalinia S. Doan L. Dokova K. Doku D.T. Dorsey E.R. Doyle K.E. Driscoll T.R. Dubey M. Dubljanin E. Duken E.E. Duncan B.B. Duraes A.R. Ebrahimi H. Ebrahimpour S. Echko M.M. Edvardsson D. Effiong A. Ehrlich J.R. El Bcheraoui C. El Sayed Zaki M. El-Khatib Z. Elkout H. Elyazar I.R.F. Enayati A. Endries A.Y. Er B. Erskine H.E. Eshrati B. Eskandarieh S. Esteghamati A. Esteghamati S. Fakhim H. Fallah Omrani V. Faramarzi M. Fareed M. Farhadi F. Farid T.A. Farinha C.S.E. Farioli A. Faro A. Farvid M.S. Farzadfar F. Feigin V.L. Fentahun N. Fereshtehnejad S-M. Fernandes E. Fernandes J.C. Ferrari A.J. Feyissa G.T. Filip I. Fischer F. Fitzmaurice C. Foigt N.A. Foreman K.J. Fox J. Frank T.D. Fukumoto T. Fullman N. Fürst T. Furtado J.M. Futran N.D. Gall S. Ganji M. Gankpe F.G. Garcia-Basteiro A.L. Gardner W.M. Gebre A.K. Gebremedhin A.T. Gebremichael T.G. Gelano T.F. Geleijnse J.M. Genova-Maleras R. Geramo Y.C.D. Gething P.W. Gezae K.E. Ghadiri K. Ghasemi Falavarjani K. Ghasemi-Kasman M. Ghimire M. Ghosh R. Ghoshal A.G. Giampaoli S. Gill P.S. Gill T.K. Ginawi I.A. Giussani G. Gnedovskaya E.V. Goldberg E.M. Goli S. Gómez-Dantés H. Gona P.N. Gopalani S.V. Gorman T.M. Goulart A.C. Goulart B.N.G. Grada A. Grams M.E. Grosso G. Gugnani H.C. Guo Y. Gupta P.C. Gupta R. Gupta R. Gupta T. Gyawali B. Haagsma J.A. Hachinski V. Hafezi-Nejad N. Haghparast Bidgoli H. Hagos T.B. Hailu G.B. Haj-Mirzaian A. Haj-Mirzaian A. Hamadeh R.R. Hamidi S. Handal A.J. Hankey G.J. Hao Y. Harb H.L. Harikrishnan S. Haro J.M. Hasan M. Hassankhani H. Hassen H.Y. Havmoeller R. Hawley C.N. Hay R.J. Hay S.I. Hedayatizadeh-Omran A. Heibati B. Hendrie D. Henok A. Herteliu C. Heydarpour S. Hibstu D.T. Hoang H.T. Hoek H.W. Hoffman H.J. Hole M.K. Homaie Rad E. Hoogar P. Hosgood H.D. Hosseini S.M. Hosseinzadeh M. Hostiuc M. Hostiuc S. Hotez P.J. Hoy D.G. Hsairi M. Htet A.S. Hu G. Huang J.J. Huynh C.K. Iburg K.M. Ikeda C.T. Ileanu B. Ilesanmi O.S. Iqbal U. Irvani S.S.N. Irvine C.M.S. Islam S.M.S. Islami F. Jacobsen K.H. Jahangiry L. Jahanmehr N. Jain S.K. Jakovljevic M. Javanbakht M. Jayatilleke A.U. Jeemon P. Jha R.P. Jha V. Ji J.S. Johnson C.O. Jonas J.B. Jozwiak J.J. Jungari S.B. Jürisson M. Kabir Z. Kadel R. Kahsay A. Kalani R. Kanchan T. Karami M. Karami Matin B. Karch A. Karema C. Karimi N. Karimi S.M. Kasaeian A. Kassa D.H. Kassa G.M. Kassa T.D. Kassebaum N.J. Katikireddi S.V. Kawakami N. Karyani A.K. Keighobadi M.M. Keiyoro P.N. Kemmer L. Kemp G.R. Kengne A.P. Keren A. Khader Y.S. Khafaei B. Khafaie M.A. Khajavi A. Khalil I.A. Khan E.A. Khan M.S. Khan M.A. Khang Y-H. Khazaei M. Khoja A.T. Khosravi A. Khosravi M.H. Kiadaliri A.A. Kiirithio D.N. Kim C-I. Kim D. Kim P. Kim Y-E. Kim Y.J. Kimokoti R.W. Kinfu Y. Kisa A. Kissimova-Skarbek K. Kivimäki M. Knudsen A.K.S. Kocarnik J.M. Kochhar S. Kokubo Y. Kolola T. Kopec J.A. Kosen S. Kotsakis G.A. Koul P.A. Koyanagi A. Kravchenko M.A. Krishan K. Krohn K.J. Kuate Defo B. Kucuk Bicer B. Kumar G.A. Kumar M. Kyu H.H. Lad D.P. Lad S.D. Lafranconi A. Lalloo R. Lallukka T. Lami F.H. Lansingh V.C. Latifi A. Lau K.M-M. Lazarus J.V. Leasher J.L. Ledesma J.R. Lee P.H. Leigh J. Leung J. Levi M. Lewycka S. Li S. Li Y. Liao Y. Liben M.L. Lim L-L. Lim S.S. Liu S. Lodha R. Looker K.J. Lopez A.D. Lorkowski S. Lotufo P.A. Low N. Lozano R. Lucas T.C.D. Lucchesi L.R. Lunevicius R. Lyons R.A. Ma S. Macarayan E.R.K. Mackay M.T. Madotto F. Magdy Abd El Razek H. Magdy Abd El Razek M. Maghavani D.P. Mahotra N.B. Mai H.T. Majdan M. Majdzadeh R. Majeed A. Malekzadeh R. Malta D.C. Mamun A.A. Manda A-L. Manguerra H. Manhertz T. Mansournia M.A. Mantovani L.G. Mapoma C.C. Maravilla J.C. Marcenes W. Marks A. Martins-Melo F.R. Martopullo I. März W. Marzan M.B. Mashamba-Thompson T.P. Massenburg B.B. Mathur M.R. Matsushita K. Maulik P.K. Mazidi M. McAlinden C. McGrath J.J. McKee M. Mehndiratta M.M. Mehrotra R. Mehta K.M. Mehta V. Mejia-Rodriguez F. Mekonen T. Melese A. Melku M. Meltzer M. Memiah P.T.N. Memish Z.A. Mendoza W. Mengistu D.T. Mengistu G. Mensah G.A. Mereta S.T. Meretoja A. Meretoja T.J. Mestrovic T. Mezerji N.M.G. Miazgowski B. Miazgowski T. Millear A.I. Miller T.R. Miltz B. Mini G.K. Mirarefin M. Mirrakhimov E.M. Misganaw A.T. Mitchell P.B. Mitiku H. Moazen B. Mohajer B. Mohammad K.A. Mohammadifard N. Mohammadnia-Afrouzi M. Mohammed M.A. Mohammed S. Mohebi F. Moitra M. Mokdad A.H. Molokhia M. Monasta L. Moodley Y. Moosazadeh M. Moradi G. Moradi-Lakeh M. Moradinazar M. Moraga P. Morawska L. Moreno Velásquez I. Morgado-Da-Costa J. Morrison S.D. Moschos M.M. Mountjoy-Venning W.C. Mousavi S.M. Mruts K.B. Muche A.A. Muchie K.F. Mueller U.O. Muhammed O.S. Mukhopadhyay S. Muller K. Mumford J.E. Murhekar M. Musa J. Musa K.I. Mustafa G. Nabhan A.F. Nagata C. Naghavi M. Naheed A. Nahvijou A. Naik G. Naik N. Najafi F. Naldi L. Nam H.S. Nangia V. Nansseu J.R. Nascimento B.R. Natarajan G. Neamati N. Negoi I. Negoi R.I. Neupane S. Newton C.R.J. Ngunjiri J.W. Nguyen A.Q. Nguyen H.T. Nguyen H.L.T. Nguyen H.T. Nguyen L.H. Nguyen M. Nguyen N.B. Nguyen S.H. Nichols E. Ningrum D.N.A. Nixon M.R. Nolutshungu N. Nomura S. Norheim O.F. Noroozi M. Norrving B. Noubiap J.J. Nouri H.R. Nourollahpour Shiadeh M. Nowroozi M.R. Nsoesie E.O. Nyasulu P.S. Odell C.M. Ofori-Asenso R. Ogbo F.A. Oh I-H. Oladimeji O. Olagunju A.T. Olagunju T.O. Olivares P.R. Olsen H.E. Olusanya B.O. Ong K.L. Ong S.K. Oren E. Ortiz A. Ota E. Otstavnov S.S. Øverland S. Owolabi M.O. P A M. Pacella R. Pakpour A.H. Pana A. Panda-Jonas S. Parisi A. Park E-K. Parry C.D.H. Patel S. Pati S. Patil S.T. Patle A. Patton G.C. Paturi V.R. Paulson K.R. Pearce N. Pereira D.M. Perico N. Pesudovs K. Pham H.Q. Phillips M.R. Pigott D.M. Pillay J.D. Piradov M.A. Pirsaheb M. Pishgar F. Plana-Ripoll O. Plass D. Polinder S. Popova S. Postma M.J. Pourshams A. Poustchi H. Prabhakaran D. Prakash S. Prakash V. Purcell C.A. Purwar M.B. Qorbani M. Quistberg D.A. Radfar A. Rafay A. Rafiei A. Rahim F. Rahimi K. Rahimi-Movaghar A. Rahimi-Movaghar V. Rahman M. Rahman M.H. Rahman M.A. Rahman S.U. Rai R.K. Rajati F. Ram U. Ranjan P. Ranta A. Rao P.C. Rawaf D.L. Rawaf S. Reddy K.S. Reiner R.C. Reinig N. Reitsma M.B. Remuzzi G. Renzaho A.M.N. Resnikoff S. Rezaei S. Rezai M.S. Ribeiro A.L.P. Roberts N.L.S. Robinson S.R. Roever L. Ronfani L. Roshandel G. Rostami A. Roth G.A. Roy A. Rubagotti E. Sachdev P.S. Sadat N. Saddik B. Sadeghi E. Saeedi Moghaddam S. Safari H. Safari Y. Safari-Faramani R. Safdarian M. Safi S. Safiri S. Sagar R. Sahebkar A. Sahraian M.A. Sajadi H.S. Salam N. Salama J.S. Salamati P. Saleem K. Saleem Z. Salimi Y. Salomon J.A. Salvi S.S. Salz I. Samy A.M. Sanabria J. Sang Y. Santomauro D.F. Santos I.S. Santos J.V. Santric Milicevic M.M. Sao Jose B.P. Sardana M. Sarker A.R. Sarrafzadegan N. Sartorius B. Sarvi S. Sathian B. Satpathy M. Sawant A.R. Sawhney M. Saxena S. Saylan M. Schaeffner E. Schmidt M.I. Schneider I.J.C. Schöttker B. Schwebel D.C. Schwendicke F. Scott J.G. Sekerija M. Sepanlou S.G. Serván-Mori E. Seyedmousavi S. Shabaninejad H. Shafieesabet A. Shahbazi M. Shaheen A.A. Shaikh M.A. Shams-Beyranvand M. Shamsi M. Shamsizadeh M. Sharafi H. Sharafi K. Sharif M. Sharif-Alhoseini M. Sharma M. Sharma R. She J. Sheikh A. Shi P. Shibuya K. Shigematsu M. Shiri R. Shirkoohi R. Shishani K. Shiue I. Shokraneh F. Shoman H. Shrime M.G. Si S. Siabani S. Siddiqi T.J. Sigfusdottir I.D. Sigurvinsdottir R. Silva J.P. Silveira D.G.A. Singam N.S.V. Singh J.A. Singh N.P. Singh V. Sinha D.N. Skiadaresi E. Slepak E.L.N. Sliwa K. Smith D.L. Smith M. Soares Filho A.M. Sobaih B.H. Sobhani S. Sobngwi E. Soneji S.S. Soofi M. Soosaraei M. Sorensen R.J.D. Soriano J.B. Soyiri I.N. Sposato L.A. Sreeramareddy C.T. Srinivasan V. Stanaway J.D. Stein D.J. Steiner C. Steiner T.J. Stokes M.A. Stovner L.J. Subart M.L. Sudaryanto A. Sufiyan M.B. Sunguya B.F. Sur P.J. Sutradhar I. Sykes B.L. Sylte D.O. Tabarés-Seisdedos R. Tadakamadla S.K. Tadesse B.T. Tandon N. Tassew S.G. Tavakkoli M. Taveira N. Taylor H.R. Tehrani-Banihashemi A. Tekalign T.G. Tekelemedhin S.W. Tekle M.G. Temesgen H. Temsah M-H. Temsah O. Terkawi A.S. Teweldemedhin M. Thankappan K.R. Thomas N. Tilahun B. To Q.G. Tonelli M. Topor-Madry R. Topouzis F. Torre A.E. Tortajada-Girbés M. Touvier M. Tovani-Palone M.R. Towbin J.A. Tran B.X. Tran K.B. Troeger C.E. Truelsen T.C. Tsilimbaris M.K. Tsoi D. Tudor Car L. Tuzcu E.M. Ukwaja K.N. Ullah I. Undurraga E.A. Unutzer J. Updike R.L. Usman M.S. Uthman O.A. Vaduganathan M. Vaezi A. Valdez P.R. Varughese S. Vasankari T.J. Venketasubramanian N. Villafaina S. Violante F.S. Vladimirov S.K. Vlassov V. Vollset S.E. Vosoughi K. Vujcic I.S. Wagnew F.S. Waheed Y. Waller S.G. Wang Y. Wang Y-P. Weiderpass E. Weintraub R.G. Weiss D.J. Weldegebreal F. Weldegwergs K.G. Werdecker A. West T.E. Whiteford H.A. Widecka J. Wijeratne T. Wilner L.B. Wilson S. Winkler A.S. Wiyeh A.B. Wiysonge C.S. Wolfe C.D.A. Woolf A.D. Wu S. Wu Y-C. Wyper G.M.A. Xavier D. Xu G. Yadgir S. Yadollahpour A. Yahyazadeh Jabbari S.H. Yamada T. Yan L.L. Yano Y. Yaseri M. Yasin Y.J. Yeshaneh A. Yimer E.M. Yip P. Yisma E. Yonemoto N. Yoon S-J. Yotebieng M. Younis M.Z. Yousefifard M. Yu C. Zadnik V. Zaidi Z. Zaman S.B. Zamani M. Zare Z. Zeleke A.J. Zenebe Z.M. Zhang K. Zhao Z. Zhou M. Zodpey S. Zucker I. Vos T. Murray C.J.L. GBD 2017 Disease and Injury Incidence and Prevalence Collaborators Global, regional, and national incidence, prevalence, and years lived with disability for 354 diseases and injuries for 195 countries and territories, 1990–2017: a systematic analysis for the Global Burden of Disease Study 2017. Lancet 2018 392 10159 1789 1858 10.1016/S0140‑6736(18)32279‑7 30496104
    [Google Scholar]
  4. Phantumvanit P. Makino Y. Ogawa H. Rugg-Gunn A. Moynihan P. Petersen P.E. Evans W. Feldens C.A. Lo E. Khoshnevisan M.H. Baez R. Varenne B. Vichayanrat T. Songpaisan Y. Woodward M. Nakornchai S. Ungchusak C. WHO Global Consultation on Public Health Intervention against Early Childhood Caries. Community Dent. Oral Epidemiol. 2018 46 3 280 287 10.1111/cdoe.12362 29380407
    [Google Scholar]
  5. Casamassimo P.S. Thikkurissy S. Edelstein B.L. Maiorini E. Beyond the dmft. J. Am. Dent. Assoc. 2009 140 6 650 657 10.14219/jada.archive.2009.0250 19491160
    [Google Scholar]
  6. Loesche W.J. Role of Streptococcus mutans in human dental decay. Microbiol. Rev. 1986 50 4 353 380 10.1128/mr.50.4.353‑380.1986 3540569
    [Google Scholar]
  7. Rupf S. Merte K. Eschrich K. Kneist S. Streptococcus sobrinus in children and its influence on caries activity. Eur. Arch. Paediatr. Dent. 2006 1 1 17 22 10.1007/BF03320810 17140523
    [Google Scholar]
  8. Aas J.A. Griffen A.L. Dardis S.R. Lee A.M. Olsen I. Dewhirst F.E. Leys E.J. Paster B.J. Bacteria of dental caries in primary and permanent teeth in children and young adults. J. Clin. Microbiol. 2008 46 4 1407 1417 10.1128/JCM.01410‑07 18216213
    [Google Scholar]
  9. Xu H. Hao W. Zhou Q. Wang W. Xia Z. Liu C. Chen X. Qin M. Chen F. Plaque bacterial microbiome diversity in children younger than 30 months with or without caries prior to eruption of second primary molars. PLoS One 2014 9 2 e89269 10.1371/journal.pone.0089269 24586647
    [Google Scholar]
  10. Takahashi N. Nyvad B. The role of bacteria in the caries process: ecological perspectives. J. Dent. Res. 2011 90 3 294 303 10.1177/0022034510379602 20924061
    [Google Scholar]
  11. Peterson J. Garges S. Giovanni M. McInnes P. Wang L. Schloss J.A. Bonazzi V. McEwen J.E. Wetterstrand K.A. Deal C. Baker C.C. Di Francesco V. Howcroft T.K. Karp R.W. Lunsford R.D. Wellington C.R. Belachew T. Wright M. Giblin C. David H. Mills M. Salomon R. Mullins C. Akolkar B. Begg L. Davis C. Grandison L. Humble M. Khalsa J. Little A.R. Peavy H. Pontzer C. Portnoy M. Sayre M.H. Starke-Reed P. Zakhari S. Read J. Watson B. Guyer M. NIH HMP Working Group The NIH Human Microbiome Project. Genome Res. 2009 19 12 2317 2323 10.1101/gr.096651.109 19819907
    [Google Scholar]
  12. Fechney J.M. Browne G.V. Prabhu N. Irinyi L. Meyer W. Hughes T. Bockmann M. Townsend G. Salehi H. Adler C.J. Preliminary study of the oral mycobiome of children with and without dental caries. J. Oral Microbiol. 2019 11 1 1536182 10.1080/20002297.2018.1536182 30598729
    [Google Scholar]
  13. Cornejo Ulloa P. van der Veen M.H. Krom B.P. Review: modulation of the oral microbiome by the host to promote ecological balance. Odontology 2019 107 4 437 448 10.1007/s10266‑019‑00413‑x 30719639
    [Google Scholar]
  14. Santacroce L. Passarelli P.C. Azzolino D. Bottalico L. Charitos I.A. Cazzolla A.P. Colella M. Topi S. Godoy F.G. D’Addona A. Oral microbiota in human health and disease: A perspective. Exp. Biol. Med. (Maywood) 2023 248 15 1288 1301 10.1177/15353702231187645 37688509
    [Google Scholar]
  15. Lamont R.J. Koo H. Hajishengallis G. The oral microbiota: dynamic communities and host interactions. Nat. Rev. Microbiol. 2018 16 12 745 759 10.1038/s41579‑018‑0089‑x 30301974
    [Google Scholar]
  16. Yamashita Y. Takeshita T. The oral microbiome and human health. J. Oral Sci. 2017 59 2 201 206 10.2334/josnusd.16‑0856 28637979
    [Google Scholar]
  17. Sampaio-Maia B. Caldas I.M. Pereira M.L. Pérez-Mongiovi D. Araujo R. The Oral Microbiome in Health and Its Implication in Oral and Systemic Diseases. Adv. Appl. Microbiol. 2016 97 171 210 10.1016/bs.aambs.2016.08.002 27926431
    [Google Scholar]
  18. Fakhruddin K.S. Ngo H.C. Samaranayake L.P. Cariogenic microbiome and microbiota of the early primary dentition: A contemporary overview. Oral Dis. 2019 25 4 982 995 10.1111/odi.12932 29969843
    [Google Scholar]
  19. Jandhyala S.M. Talukdar R. Subramanyam C. Vuyyuru H. Sasikala M. Nageshwar Reddy D. Role of the normal gut microbiota. World J. Gastroenterol. 2015 21 29 8787 8803 10.3748/wjg.v21.i29.8787 26269668
    [Google Scholar]
  20. Clarke G. Sandhu K.V. Griffin B.T. Dinan T.G. Cryan J.F. Hyland N.P. Gut Reactions: Breaking Down Xenobiotic–Microbiome Interactions. Pharmacol. Rev. 2019 71 2 198 224 10.1124/pr.118.015768 30890566
    [Google Scholar]
  21. Johnson K.V.A. Foster K.R. Why does the microbiome affect behaviour? Nat. Rev. Microbiol. 2018 16 10 647 655 10.1038/s41579‑018‑0014‑3 29691482
    [Google Scholar]
  22. Rodrigues J.Z.S. Passos M.R. Silva de Macêdo Neres N. Almeida R.S. Pita L.S. Santos I.A. Santana Silveira P.H. Reis M.M. Santos I.P. de Oliveira Negrão Ricardo L. Lima B.O. D’Orleans Farias Marinho P. Soares A.B. Silva Bastos Andrade L.O. Brasileiro Pessoa S.M. Leles Silva M.M. Oliveira M.C. Pinheiro da Silva J. Moura M.A. Cruz M.P. Marques L.M. Santos T.T. Pires P.N. Teixeira Dias J.C. Rezende R.P. Trovatti Uetanabaro A.P. Yatsuda R. Antimicrobial activity of Lactobacillus fermentum TcUESC01 against Streptococcus mutans UA159. Microb. Pathog. 2020 142 104063 10.1016/j.micpath.2020.104063 32061821
    [Google Scholar]
  23. Homayouni Rad A. Pourjafar H. Mirzakhani E. A comprehensive review of the application of probiotics and postbiotics in oral health. Front. Cell. Infect. Microbiol. 2023 13 1120995 10.3389/fcimb.2023.1120995 36968114
    [Google Scholar]
  24. Mahasneh S. Mahasneh A. Probiotics: A Promising Role in Dental Health. Dent. J. 2017 5 4 26 10.3390/dj5040026 29563432
    [Google Scholar]
  25. Cannon M.L. Vorachek A. Le C. White K. Retrospective Review of Oral Probiotic Therapy. J. Clin. Pediatr. Dent. 2019 43 6 367 371 10.17796/1053‑4625‑43.6.1 31657987
    [Google Scholar]
  26. Di Pierro F. Zanvit A. Nobili P. Risso P. Fornaini C. Cariogram outcome after 90 days of oral treatment with Streptococcus salivarius M18 in children at high risk for dental caries: results of a randomized, controlled study. Clin. Cosmet. Investig. Dent. 2015 7 107 113 10.2147/CCIDE.S93066 26491371
    [Google Scholar]
  27. Zheng H. Xie T. Li S. Qiao X. Lu Y. Feng Y. Analysis of oral microbial dysbiosis associated with early childhood caries. BMC Oral Health 2021 21 1 181 10.1186/s12903‑021‑01543‑x 33827534
    [Google Scholar]
  28. Oral health surveys: basic methods - 5th edition. 2013 Available from: https://www.who.int/publications/i/item/9789241548649(accessed on 21-9-2024)
  29. Ismail A.I. Pitts N.B. Tellez M. Banerjee A. Deery C. Douglas G. Eggertsson H. Ekstrand K. Ellwood R. Gomez J. Jablonski-Momeni A. Kolker J. Longbottom C. Manton D. Martignon S. McGrady M. Rechmann P. Ricketts D. Sohn W. Thompson V. Twetman S. Weyant R. Wolff M. Zandona A. Authors of International Caries Classification and Management System (ICCMS) The International Caries Classification and Management System (ICCMS™) An Example of a Caries Management Pathway. BMC Oral Health 2015 15 S1 Suppl. 1 S9 10.1186/1472‑6831‑15‑S1‑S9 26391116
    [Google Scholar]
  30. Bárcena García M. Cobo Plana J.M. Rodríguez Cagiao G. Arcos González P.I. Epidemiological methods used in the periodontal health research in military personnel: a systematic review. BMJ Mil. Health 2024 170 1 72 77 10.1136/bmjmilitary‑2021‑001977 34921095
    [Google Scholar]
  31. Luo T. Srinivasan U. Ramadugu K. Shedden K.A. Neiswanger K. Trumble E. Li J.J. McNeil D.W. Crout R.J. Weyant R.J. Marazita M.L. Foxman B. Effects of Specimen Collection Methodologies and Storage Conditions on the Short-Term Stability of Oral Microbiome Taxonomy. Appl. Environ. Microbiol. 2016 82 18 5519 5529 10.1128/AEM.01132‑16 27371581
    [Google Scholar]
  32. Zaura E. Pappalardo V.Y. Buijs M.J. Volgenant C.M.C. Brandt B.W. Optimizing the quality of clinical studies on oral microbiome: A practical guide for planning, performing, and reporting. Periodontol. 2000 2021 85 1 210 236 10.1111/prd.12359 33226702
    [Google Scholar]
  33. Xu L. Wu Z. Wang Y. Wang S. Shu C. Duan Z. Deng S. High-throughput sequencing identifies salivary microbiota in Chinese caries-free preschool children with primary dentition. J. Zhejiang Univ. Sci. B 2021 22 4 285 294 10.1631/jzus.B2000554 33835762
    [Google Scholar]
  34. Chen W. Jiang Q. Yan G. Yang D. The oral microbiome and salivary proteins influence caries in children aged 6 to 8 years. BMC Oral Health 2020 20 1 295 10.1186/s12903‑020‑01262‑9 33115458
    [Google Scholar]
  35. Yang F. Zeng X. Ning K. Liu K.L. Lo C.C. Wang W. Chen J. Wang D. Huang R. Chang X. Chain P.S. Xie G. Ling J. Xu J. Saliva microbiomes distinguish caries-active from healthy human populations. ISME J. 2012 6 1 1 10 10.1038/ismej.2011.71 21716312
    [Google Scholar]
  36. Chen Z. Radjabzadeh D. Chen L. Kurilshikov A. Kavousi M. Ahmadizar F. Ikram M.A. Uitterlinden A.G. Zhernakova A. Fu J. Kraaij R. Voortman T. Association of Insulin Resistance and Type 2 Diabetes With Gut Microbial Diversity. JAMA Netw. Open 2021 4 7 e2118811 10.1001/jamanetworkopen.2021.18811 34323983
    [Google Scholar]
  37. Lo T.T. Van Lissa C.J. Verhagen M. Hoemann K. Erbaş Y. Maciejewski D.F. A theory-informed emotion regulation variability index: Bray–Curtis dissimilarity. Emotion 2024 24 5 1273 1285 10.1037/emo0001344 38358696
    [Google Scholar]
  38. Lif Holgerson P. Öhman C. Rönnlund A. Johansson I. Maturation of Oral Microbiota in Children with or without Dental Caries. PLoS One 2015 10 5 e0128534 10.1371/journal.pone.0128534 26020247
    [Google Scholar]
  39. Parisotto T.M. Steiner-Oliveira C. Silva C.M. Rodrigues L.K. Nobre-dos-Santos M. Early childhood caries and mutans streptococci: a systematic review. Oral Health Prev. Dent. 2010 8 1 59 70 20480056
    [Google Scholar]
  40. Hughes C.V. Dahlan M. Papadopolou E. Loo C.Y. Pradhan N.S. Lu S.C. Mathney J.M. Bravoco A. Kent R.L. Jr Tanner A.C. Aciduric microbiota and mutans streptococci in severe and recurrent severe early childhood caries. Pediatr. Dent. 2012 34 2 e16 e23 22583872
    [Google Scholar]
  41. Takahashi Y. Takashima E. Shimazu K. Yagishita H. Aoba T. Konishi K. Contribution of sialic acid-binding adhesin to pathogenesis of experimental endocarditis caused by Streptococcus gordonii DL1. Infect. Immun. 2006 74 1 740 743 10.1128/IAI.74.1.740‑743.2006 16369032
    [Google Scholar]
  42. Belstrøm D. Fiehn N.E. Nielsen C.H. Holmstrup P. Kirkby N. Klepac-Ceraj V. Paster B.J. Twetman S. Altered bacterial profiles in saliva from adults with caries lesions: a case-cohort study. Caries Res. 2014 48 5 368 375 10.1159/000357502 24643218
    [Google Scholar]
  43. Xu L. Chen X. Wang Y. Jiang W. Wang S. Ling Z. Chen H. Dynamic Alterations in Salivary Microbiota Related to Dental Caries and Age in Preschool Children With Deciduous Dentition: A 2-Year Follow-Up Study. Front. Physiol. 2018 9 342 10.3389/fphys.2018.00342 29670544
    [Google Scholar]
  44. Johansson I. Witkowska E. Kaveh B. Lif Holgerson P. Tanner A.C.R. The Microbiome in Populations with a Low and High Prevalence of Caries. J. Dent. Res. 2016 95 1 80 86 10.1177/0022034515609554 26442950
    [Google Scholar]
  45. Parker B.J. Wearsch P.A. Veloo A.C.M. Rodriguez-Palacios A. The Genus. Front. Immunol. 2020 11 906 10.3389/fimmu.2020.00906 32582143
    [Google Scholar]
  46. Ulsemer P. Toutounian K. Schmidt J. Karsten U. Goletz S. Preliminary safety evaluation of a new Bacteroides xylanisolvens isolate. Appl. Environ. Microbiol. 2012 78 2 528 535 10.1128/AEM.06641‑11 22101046
    [Google Scholar]
  47. Duranti S. Ruiz L. Lugli G.A. Tames H. Milani C. Mancabelli L. Mancino W. Longhi G. Carnevali L. Sgoifo A. Margolles A. Ventura M. Ruas-Madiedo P. Turroni F. Bifidobacterium adolescentis as a key member of the human gut microbiota in the production of GABA. Sci. Rep. 2020 10 1 14112 10.1038/s41598‑020‑70986‑z 32839473
    [Google Scholar]
  48. Malinen E. Krogius-Kurikka L. Lyra A. Nikkilä J. Jääskeläinen A. Rinttilä T. Vilpponen-Salmela T. von Wright A.J. Palva A. Association of symptoms with gastrointestinal microbiota in irritable bowel syndrome. World J. Gastroenterol. 2010 16 36 4532 4540 10.3748/wjg.v16.i36.4532 20857523
    [Google Scholar]
  49. Belstrøm D. Holmstrup P. Fiehn N.E. Kirkby N. Kokaras A. Paster B.J. Bardow A. Salivary microbiota in individuals with different levels of caries experience. J. Oral Microbiol. 2017 9 1 1270614 10.1080/20002297.2016.1270614 28326153
    [Google Scholar]
  50. Yousuf B. Mishra A. Exploring human bacterial diversity toward prevention of infectious disease and health promotion. Microbial Diversity in the Genomic Era. Das S. Dash H.R. Academic Press 2019 519 533 10.1016/B978‑0‑12‑814849‑5.00029‑0
    [Google Scholar]
  51. DeGruttola A.K. Low D. Mizoguchi A. Mizoguchi E. Current understanding of dysbiosis in disease in human and animal models. Inflamm. Bowel Dis. 2016 22 5 1137 1150 10.1097/MIB.0000000000000750 27070911
    [Google Scholar]
  52. Fiorucci S. Carino A. Baldoni M. Santucci L. Costanzi E. Graziosi L. Distrutti E. Biagioli M. Bile Acid Signaling in Inflammatory Bowel Diseases. Dig. Dis. Sci. 2021 66 3 674 693 10.1007/s10620‑020‑06715‑3 33289902
    [Google Scholar]
  53. Dewhirst F.E. Chen T. Izard J. Paster B.J. Tanner A.C.R. Yu W.H. Lakshmanan A. Wade W.G. The human oral microbiome. J. Bacteriol. 2010 192 19 5002 5017 10.1128/JB.00542‑10 20656903
    [Google Scholar]
  54. Jiang S. Gao X. Jin L. Lo E. Salivary Microbiome Diversity in Caries-Free and Caries-Affected Children. Int. J. Mol. Sci. 2016 17 12 1978 10.3390/ijms17121978 27898021
    [Google Scholar]
  55. Xiao C. Ran S. Huang Z. Liang J. Bacterial Diversity and Community Structure of Supragingival Plaques in Adults with Dental Health or Caries Revealed by 16S Pyrosequencing. Front. Microbiol. 2016 7 1145 10.3389/fmicb.2016.01145 27499752
    [Google Scholar]
  56. Costalonga M. Herzberg M.C. The oral microbiome and the immunobiology of periodontal disease and caries. Immunol. Lett. 2014 162 2 22 38 10.1016/j.imlet.2014.08.017 25447398
    [Google Scholar]
  57. Agnello M. Marques J. Cen L. Mittermuller B. Huang A. Chaichanasakul Tran N. Shi W. He X. Schroth R.J. Microbiome Associated with Severe Caries in Canadian First Nations Children. J. Dent. Res. 2017 96 12 1378 1385 10.1177/0022034517718819 28709393
    [Google Scholar]
  58. Crielaard W. Zaura E. Schuller A.A. Huse S.M. Montijn R.C. Keijser B.J.F. Exploring the oral microbiota of children at various developmental stages of their dentition in the relation to their oral health. BMC Med. Genomics 2011 4 1 22 10.1186/1755‑8794‑4‑22 21371338
    [Google Scholar]
  59. Liu G. Wu C. Abrams W.R. Li Y. Structural and Functional Characteristics of the Microbiome in Deep-Dentin Caries. J. Dent. Res. 2020 99 6 713 720 10.1177/0022034520913248 32196394
    [Google Scholar]
  60. Hurley E. Barrett M.P.J. Kinirons M. Whelton H. Ryan C.A. Stanton C. Harris H.M.B. O’Toole P.W. Comparison of the salivary and dentinal microbiome of children with severe-early childhood caries to the salivary microbiome of caries-free children. BMC Oral Health 2019 19 1 13 10.1186/s12903‑018‑0693‑1 30642327
    [Google Scholar]
  61. Becker M.R. Paster B.J. Leys E.J. Moeschberger M.L. Kenyon S.G. Galvin J.L. Boches S.K. Dewhirst F.E. Griffen A.L. Molecular analysis of bacterial species associated with childhood caries. J. Clin. Microbiol. 2002 40 3 1001 1009 10.1128/JCM.40.3.1001‑1009.2002 11880430
    [Google Scholar]
  62. Gross E.L. Leys E.J. Gasparovich S.R. Firestone N.D. Schwartzbaum J.A. Janies D.A. Asnani K. Griffen A.L. Bacterial 16S sequence analysis of severe caries in young permanent teeth. J. Clin. Microbiol. 2010 48 11 4121 4128 10.1128/JCM.01232‑10 20826648
    [Google Scholar]
  63. Goret J. Baudinet T. Camou F. Issa N. Gaillard P. Wirth G. Greib C. Barandon L. Mégraud F. Bébéar C. Peuchant O. Ménard A. Identification of Streptococcus sinensis from a patient with endocarditis using MALDI-TOF mass spectrometry, 16S rDNA- and sodA-based phylogeny. J. Microbiol. Immunol. Infect. 2019 52 3 507 509 10.1016/j.jmii.2018.04.004 29891358
    [Google Scholar]
  64. Spatafora G. Li Y. He X. Cowan A. Tanner A.C.R. The Evolving Microbiome of Dental Caries. Microorganisms 2024 12 1 121 10.3390/microorganisms12010121 38257948
    [Google Scholar]
  65. Meriç E. Bolgül B. Duran N. Ay E. Evaluation of oral streptococci in saliva of children with severe Early Childhood Caries and caries-free. Eur. J. Paediatr. Dent. 2020 21 1 13 17 10.23804/ejpd.2020.21.01.03 32183522
    [Google Scholar]
  66. Anderson A.C. Rothballer M. Altenburger M.J. Woelber J.P. Karygianni L. Lagkouvardos I. Hellwig E. Al-Ahmad A. In-vivo shift of the microbiota in oral biofilm in response to frequent sucrose consumption. Sci. Rep. 2018 8 1 14202 10.1038/s41598‑018‑32544‑6 30242260
    [Google Scholar]
  67. Martín V. Mañes-Lázaro R. Rodríguez J.M. Maldonado-Barragán A. Streptococcus lactarius sp. nov., isolated from breast milk of healthy women. Int. J. Syst. Evol. Microbiol. 2011 61 5 1048 1052 10.1099/ijs.0.021642‑0 20511454
    [Google Scholar]
  68. Tapiainen T. Paalanne N. Arkkola T. Renko M. Pokka T. Kaijalainen T. Uhari M. Diet as a risk factor for pneumococcal carriage and otitis media: a cross-sectional study among children in day care centers. PLoS One 2014 9 3 e90585 10.1371/journal.pone.0090585 24599395
    [Google Scholar]
  69. Kistler J.O. Booth V. Bradshaw D.J. Wade W.G. Bacterial community development in experimental gingivitis. PLoS One 2013 8 8 e71227 10.1371/journal.pone.0071227 23967169
    [Google Scholar]
  70. Wang Y. Zhang J. Chen X. Jiang W. Wang S. Xu L. Tu Y. Zheng P. Wang Y. Lin X. Chen H. Profiling of oral microbiota in early childhood caries using single-molecule real-time sequencing. Front. Microbiol. 2017 8 2244 10.3389/fmicb.2017.02244 29187843
    [Google Scholar]
  71. Rawlinson A. Duerden B.I. Goodwin L. New findings on the microbial flora associated with adult periodontitis. J. Dent. 1993 21 3 179 184 10.1016/0300‑5712(93)90029‑P 8315093
    [Google Scholar]
  72. Loozen G. Boon N. Pauwels M. Slomka V. Rodrigues Herrero E. Quirynen M. Teughels W. Effect of Bdellovibrio bacteriovorus HD100 on multispecies oral communities. Anaerobe 2015 35 Pt A 45 53 10.1016/j.anaerobe.2014.09.011 25252124
    [Google Scholar]
  73. Seminario-Amez M. López-López J. Estrugo-Devesa A. Ayuso-Montero R. Jané-Salas E. Probiotics and oral health: A systematic review. Med. Oral Patol. Oral Cir. Bucal 2017 22 3 0 10.4317/medoral.21494 28390121
    [Google Scholar]
  74. Pfaffe T. Cooper-White J. Beyerlein P. Kostner K. Punyadeera C. Diagnostic potential of saliva: current state and future applications. Clin. Chem. 2011 57 5 675 687 10.1373/clinchem.2010.153767 21383043
    [Google Scholar]
  75. Yang Y. Weng W. Peng J. Hong L. Yang L. Toiyama Y. Gao R. Liu M. Yin M. Pan C. Li H. Guo B. Zhu Q. Wei Q. Moyer M.P. Wang P. Cai S. Goel A. Qin H. Ma Y. Fusobacterium nucleatum increases proliferation of colorectal cancer cells and tumor development in mice by activating toll-like receptor 4 signaling to nuclear factor−κb, and up-regulating expression of microRNA-21. Gastroenterology 2017 152 4 851 866.e24 10.1053/j.gastro.2016.11.018 27876571
    [Google Scholar]
  76. Kim M. Friesen L. Park J. Kim H.M. Kim C.H. Microbial metabolites, short‐chain fatty acids, restrain tissue bacterial load, chronic inflammation, and associated cancer in the colon of mice. Eur. J. Immunol. 2018 48 7 1235 1247 10.1002/eji.201747122 29644622
    [Google Scholar]
/content/journals/raiad/10.2174/0127722708335159241117062704
Loading
Analysis of Oral and Gut Microbiota Composition in Children with Dental Caries by NGS Approaches
Bentham Science Publishers ; https://doi.org/10.2174/0127722708335159241117062704
/content/journals/raiad/10.2174/0127722708335159241117062704
/content/journals/raiad/10.2174/0127722708335159241117062704
Loading

Data & Media loading...

Supplements

Supplementary material is available on the publisher's website along with the published article.

file format download Download

  • Article Type:     Research Article
Keywords: Paediatric dentistry ; next generation sequencing ; molecular biology ; probiotics ; microbial ecology

Most Cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error
Please enter a valid_number test