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2000
Volume 19, Issue 3
  • ISSN: 2772-2708
  • E-ISSN: 2772-2716

Abstract

Background

Sepsis is a life-threatening condition responsible for high morbidity and mortality rates around the world and is characterized by a dysregulated host response to infection, resulting in multiple organ dysfunctions. Eugenol is a phenolic aromatic compound derived from clove oil. It has anti-inflammatory, antioxidant, antibacterial, antiviral, antifungal, and anticancer characteristics, which have led to its extensive use in diverse fields, including cosmetology, medicine, and pharmacology. The ongoing study aimed to evaluate the efficacy of eugenol-loaded chitosan nanoparticles (EC-NPs) on sepsis-induced liver damage using the cecal ligation and puncture (CLP) model.

Methods

Thirty male albino rats were randomly divided into five groups: Sham, sepsis, and septic rats treated with chitosan, eugenol, or EC-NPs.

Results

EC-NPs showed excellent antibacterial, antioxidant, and anti-inflammatory effects . EC-NPs administration significantly improved liver function, as indicated by the decreased liver enzyme activities and C-reactive protein (CRP) level, as well as the increase of albumin content. Moreover, EC-NPs caused an increase in glutathione-reduced and antioxidant enzymes activities, as well as a reduction of malondialdehyde and nitric oxide formation. In addition, the EC-NPs treatment reduced the DNA damage in septic rats; also, the EC-NPs treatment repaired, to some extent, the abnormal architecture of the hepatic tissues of septic rats. Furthermore, the immunohistochemical examination showed a marked decrease in inflammation through the reduction of TNF-α and IL-1β expression.

Conclusion

In conclusion, EC-NPs attenuated liver injury in sepsis through their anti-inflammatory, anti-bacterial, anti-oxidant activities and protection of DNA.

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Efficacy of Eugenol Loaded Chitosan Nanoparticles on Sepsis Induced Liver Injury in Rats
Rec Adv Inflamm Allergy Drug Discov 19, 380 (2025); https://doi.org/10.2174/0127722708334976241004041438
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  • Article Type:     Research Article
Keyword(s): cecal ligation and puncture; chitosan nanoparticles; Eugenol; hepatic injury; oxidative stress; sepsis

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