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image of An Assessment of BHLHE40 Transcription Factor Level and its Target Cytokines in Patients with Rheumatoid Arthritis

Abstract

Basic helix-loop-helix protein 40 (BHLHE40) can function as both a transcriptional activator and repressor. Recent studies have reported its regulatory functions in T helper (Th)1 and Th17 immune responses. Rheumatoid arthritis (RA) is an autoimmune inflammatory disease in which joints are involved. Both Th1 and Th17 contribute to the pathogenesis of the disease. In the present study, the levels of BHLHE40, interleukin 17 (), and interferon-gamma () were assessed in the RA patients.

Two groups, including RA patients and healthy individuals, were included in the study. The relative expression levels of BHLHE40, , and were quantified in peripheral blood mononuclear cells (PBMCs) using real-time PCR.

The results showed that the level of was significantly higher in RA patients compared to the healthy control ( < 0.001) (11.1-fold increase). Accordingly, a significant increase in the levels of (8.1 folds increase) ( < 0.021) and (12.7 folds) ( < 0.001) was observed.

Evaluation of the expression level of in RA patients showed a significant increase. In line with the elevated level of BHLHE40, a significant increase in the expression level of and was also detected. These findings point to the possible role of BHLHE40 in the disease course or severity by elevating the levels of inflammatory cytokines, including and . Therefore, BHLHE40 might be considered either as a putative biomarker or as a candidate for therapy in a variety of human diseases.

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/content/journals/raiad/10.2174/0127722708331558250129152044
2025-02-04
2025-06-20

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/content/journals/raiad/10.2174/0127722708331558250129152044
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An Assessment of BHLHE40 Transcription Factor Level and its Target Cytokines in Patients with Rheumatoid Arthritis
Bentham Science Publishers ; https://doi.org/10.2174/0127722708331558250129152044
/content/journals/raiad/10.2174/0127722708331558250129152044
/content/journals/raiad/10.2174/0127722708331558250129152044
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  • Article Type:     Research Article
Keywords: BHLHE40 ; IL-17 ; rheumatoid arthritis ; IFN-γ

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