Network Pharmacology Analysis to Explore the Pharmacological Mechanism of Cang Er Zi Powder Against Allergic Rhinitis

Qisheng Cui; Ping Liu; Huifang He; Cuiyin Chen; Xuemin Hou; Tao Xie; Weijie Chen

doi:10.2174/0127722708290725241022064414

image of Network Pharmacology Analysis to Explore the Pharmacological Mechanism of Cang Er Zi Powder Against Allergic Rhinitis

Network Pharmacology Analysis to Explore the Pharmacological Mechanism of Cang Er Zi Powder Against Allergic Rhinitis
Authors: Qisheng Cui¹, Ping Liu², Huifang He¹, Cuiyin Chen¹, Xuemin Hou¹, Tao Xie³ and Weijie Chen¹
View Affiliations Hide Affiliations

Affiliations: ¹ Department of Rehabilitation Medicine, Hexian Memorial Affiliated Hospital of Southern Medical University, Guangzhou, Guangdong, China ; ² Department of Chinese Traditional Medicine, Zhong Cui Block Community Health Service center of Panyu, Guangzhou, Guangdong, China ; ³ Department of Radiation Oncology, Affiliated Cancer Hospital and Institute of Guangzhou Medical University, Guangzhou Province, People’s Republic of China
Source: Recent Advances in Inflammation & Allergy Drug Discovery
Available online: 06 November 2024
DOI: https://doi.org/10.2174/0127722708290725241022064414
- Received: 27 Dec 2023
- Accepted: 20 Sep 2024
- Available online: 06 Nov 2024

Abstract

Background

Allergic rhinitis (AR) is a leading public health problem with high prevalence, but the therapies remain limited. Cang Er Zi Powder (CEZP), a Traditional Chinese Medicine formula, has been used for the clinical treatment of chronic rhinitis and allergic rhinitis in China for decades. However, the underlying mechanism is unclear.

Objective

In this study, we aimed to clarify the pharmacological mechanism of CEZP on allergic rhinitis

Method

The active ingredients of CEZP were screened in the TCMSP (http://tcmspw.com/tcmsp.php) database. The targets related to “allergic rhinitis” were retrieved from MALACARDS, TTD, and DisGeNET disease target databases. The active ingredients and the candidate targets for AR were constructed and visualized using Cytoscape 3.7.2 software. The underlying mechanism involved in the treatment of CP against AR was analyzed using the WEB-based GEne SeT AnaLysis Toolkit. The effects of CEZP on levels of β-hexosaminidase, histamine, interleukin (IL)-4, and tumor necrosis factor (TNF)-α on DNP-IgE/HSA-stimulated rat basophilic leukemia cells were determined by enzyme-linked immunosorbent assay (ELISA) kits.

Results

A total of 78 active ingredients in 9 Chinese herbs of CEZP and 90 target overlap targets from CEZP and AR were identified. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis suggested that the inflammation response and NF-κB signaling pathway were responsible for the therapeutic targets of CEZP on AR, and CEZP could suppress mast cell degranulation via Toll-like receptor (TLR) and NF-κb signaling pathway.

Conclusion

Network pharmacology analysis and in vitro assays suggested that CEZP may exert therapeutic effects on AR by inhibiting the NF-κB signaling pathways.

Article metrics loading...

/content/journals/raiad/10.2174/0127722708290725241022064414

2024-11-06

2025-01-20

From This Site

/content/journals/raiad/10.2174/0127722708290725241022064414

dcterms_title,dcterms_subject,pub_keyword

-contentType:Contributor -contentType:Concept -contentType:Institution

10

5

Full text loading...

References

Varshney J. Varshney H. Allergic rhinitis: An overview. Indian J. Otolaryngol. Head Neck Surg. 2015 67 143 149 10.1007/s12070‑015‑0828‑5
[Google Scholar]
Min Y.G. The pathophysiology, diagnosis and treatment of allergic rhinitis. Allergy Asthma Immunol. Res. 2010 2 2 65 76 10.4168/aair.2010.2.2.65 20358020
[Google Scholar]
Cvetkovski B. Tan R. Kritikos V. A patient-centric analysis to identify key influences in allergic rhinitis management. NPJ Prim. Care Respir. Med. 2018 28 1 34 10.1038/s41533‑018‑0100‑z 30213945
[Google Scholar]
Zhao Y. van Hasselt C.A. Woo J.K. Effect of a Chinese herbal formula, Shi-Bi-Lin, on an experimental model of allergic rhinitis. Ann. Allergy Asthma Immunol. 2006 96 6 844 850 10.1016/S1081‑1206(10)61348‑8 16802773
[Google Scholar]
Zhao Y. Woo K.S. Ma K.H. Treatment of perennial allergic rhinitis using Shi-Bi-Lin, a Chinese herbal formula. J. Ethnopharmacol. 2009 122 1 100 105 10.1016/j.jep.2008.12.005 19118617
[Google Scholar]
Zhang W. Huai Y. Miao Z. Qian A. Wang Y. Systems pharmacology for investigation of the mechanisms of action of traditional Chinese medicine in drug discovery. Front. Pharmacol. 2019 10 743 10.3389/fphar.2019.00743 31379563
[Google Scholar]
Hopkins A.L. Network pharmacology: The next paradigm in drug discovery. Nat. Chem. Biol. 2008 4 11 682 690 10.1038/nchembio.118 18936753
[Google Scholar]
Ru J. Li P. Wang J. Zhou W. Li B. Huang C. Li P. Guo Z. Tao W. Yang Y. Xu X. Li Y. Wang Y. Yang L. TCMSP: A database of systems pharmacology for drug discovery from herbal medicines. J. Cheminform. 2014 6 1 13 10.1186/1758‑2946‑6‑13 24735618
[Google Scholar]
Rappaport N. Twik M. Plaschkes I. MalaCards: An amalgamated human disease compendium with diverse clinical and genetic annotation and structured search. Nucleic Acids Res. 2017 45 D1 D877 D887 10.1093/nar/gkw1012 27899610
[Google Scholar]
Wang Y. Zhang S. Li F. Therapeutic target database 2020: Enriched resource for facilitating research and early development of targeted therapeutics. Nucleic Acids Res. 2020 48 D1 D1031 D1041 31691823
[Google Scholar]
Piñero J. Bravo À. Queralt-Rosinach N. DisGeNET: A comprehensive platform integrating information on human disease-associated genes and variants. Nucleic Acids Res. 2017 45 D1 D833 D839 10.1093/nar/gkw943 27924018
[Google Scholar]
Chen L. Cao Y. Zhang H. Network pharmacology-based strategy for predicting active ingredients and potential targets of Yangxinshi tablet for treating heart failure. J. Ethnopharmacol. 2018 219 359 368 10.1016/j.jep.2017.12.011 29366769
[Google Scholar]
Liao Y. Wang J. Jaehnig E.J. Shi Z. Zhang B. WebGestalt 2019: Gene set analysis toolkit with revamped UIs and APIs. Nucleic Acids Res. 2019 47 W1 W199 W205 10.1093/nar/gkz401 31114916
[Google Scholar]
Zaugg J. Eickmeier E. Rueda D.C. Hering S. Hamburger M. HPLC-based activity profiling of Angelica pubescens roots for new positive GABAA receptor modulators in Xenopus oocytes. Fitoterapia 2011 82 3 434 440 10.1016/j.fitote.2010.12.001 21147202
[Google Scholar]
Wang C.C. Lai J.E. Chen L.G. Yen K.Y. Yang L.L. Inducible nitric oxide synthase inhibitors of Chinese herbs. Part 2: Naturally occurring furanocoumarins. Bioorg. Med. Chem. 2000 8 12 2701 2707 10.1016/S0968‑0896(00)00200‑5 11131161
[Google Scholar]
Lin C.H. Chang C.W. Wang C.C. Chang M.S. Yang L.L. Byakangelicol, isolated from Angelica dahurica, inhibits both the activity and induction of cyclooxygenase-2 in human pulmonary epithelial cells. J. Pharm. Pharmacol. 2010 54 9 1271 1278 10.1211/002235702320402125 12356282
[Google Scholar]
Yokozawa T. Tanaka T. Kimura T. Examination of the nitric oxide production-suppressing component in Tinospora tuberculata. Biol. Pharm. Bull. 2001 24 10 1153 1156 10.1248/bpb.24.1153 11642322
[Google Scholar]
Lee S.H. Veeriah V. Levine F. Liver fat storage is controlled by HNF4α through induction of lipophagy and is reversed by a potent HNF4α agonist. Cell Death Dis. 2021 12 6 603 10.1038/s41419‑021‑03862‑x 34117215
[Google Scholar]
Jiang Y. Yu L. Wang M.H. N-trans-feruloyltyramine inhibits LPS-induced NO and PGE2 production in RAW 264.7 macrophages: Involvement of AP-1 and MAP kinase signalling pathways. Chem. Biol. Interact. 2015 235 56 62 10.1016/j.cbi.2015.03.029 25843058
[Google Scholar]
Redhu N.S. Saleh A. Shan L. Proinflammatory and Th2 cytokines regulate the high affinity IgE receptor (FcepsilonRI) and IgE-dependant activation of human airway smooth muscle cells. PLoS One 2009 4 7 e6153 10.1371/journal.pone.0006153 19582151
[Google Scholar]
Lin X. Lv J. Ge D. Heme oxygenase-1 alleviates eosinophilic inflammation by inhibiting STAT3-SOCS3 signaling. Pediatr. Pulmonol. 55 6 1440 1447 2020 10.1002/ppul.24759 32297710
[Google Scholar]
Santana F.P.R. da Silva R.C. Grecco S.S. Inhibition of MAPK and STAT3-SOCS3 by sakuranetin attenuated chronic allergic airway inflammation in mice. Mediators Inflamm. 2019 2019 1 14 10.1155/2019/1356356 31565031
[Google Scholar]
Wu Z. Mehrabi Nasab E. Arora P. Athari S.S. Study effect of probiotics and prebiotics on treatment of OVA-LPS-induced of allergic asthma inflammation and pneumonia by regulating the TLR4/NF-kB signaling pathway. J. Transl. Med. 2022 20 1 130 10.1186/s12967‑022‑03337‑3 35296330
[Google Scholar]
Iannucci A. Caneparo V. Raviola S. Toll-like receptor 4-mediated inflammation triggered by extracellular IFI16 is enhanced by lipopolysaccharide binding. PLoS Pathog. 2020 16 9 e1008811 10.1371/journal.ppat.1008811 32903274
[Google Scholar]
Zhang D. Liu B. Jie X. Uncovering bupi yishen formula pharmacological mechanisms against chronic kidney disease by network pharmacology and experimental validation. Front. Pharmacol. 2021 12 761572 10.3389/fphar.2021.761572 34867380
[Google Scholar]
Li H. Kreiner J.M. Wong A.R. Oral application of Chinese herbal medicine for allergic rhinitis: A systematic review and meta‐analysis of randomized controlled trials. Phytother. Res. 2021 35 6 3113 3129 10.1002/ptr.7037 33533107
[Google Scholar]