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image of Growth Promotion and In vitro Seed Germination of Lycium barbarum L. (Red Goji) Using Different Types of Cytokinins

Abstract

Introduction

L., commonly known as red goji berry, is a widely recognized plant-based medicinal herb with nutritional and therapeutic properties. In this study, the effects of various cytokinins on the germination of seeds and the growth of seedlings were investigated under conditions.

Methods

The berries were first surface sterilized and dissected, and the seeds were then cultured on Murashige and Skoog (MS) medium supplemented with different concentrations (0.5, 1.0, and 1.5 mg/L) of 6-benzylaminopurine (BAP), thidiazuron (TDZ), and kinetin (KIN) for 10 weeks at 25±2ºC with a photoperiod of 16 hours and a light intensity of 1000 lux. Upon observation after 10 weeks of culture, all cytokinin-treated cultures produced 100% seed germination as early as 7 days. KIN at 0.5 mg/L produced plantlets with the greatest height (8.40 ± 0.97 cm) with extensive rooting and the greatest total chlorophyll production. Besides, KIN at 1.5 mg/L resulted in the highest number of leaves per plantlet (6.90 ± 0.72), while 1.0 mg/L of TDZ led to the greatest biomass, i.e., fresh weight (FW) of 0.328 ± 0.05 gram and dry weight (DW) of 0.023 ± 0.003 gram.

Results

All cytokinins used in this experiment (BAP, TDZ, and KIN) promoted different growth promotion responses in . The effects of different types and concentrations of cytokinin on the height of plantlets, number of leaves per plantlet, fresh and dry weight, the extent of rooting, and the chlorophyll content were demonstrated to be statistically significant.

Conclusion

This study provides valuable insights into optimizing cultivation techniques for goji berry propagation, which could contribute to developing superior cultivars and increased production of this superfruit in the future. For future perspectives, extended research in elucidating the underlying mechanism associated with cytokinin supplementation is imperative in understanding the roles of cytokinins and optimizing their effects on plant growth promotion.

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2024-10-11
2024-11-18

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/content/journals/rafna/10.2174/012772574X313421240902052600
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Growth Promotion and In vitro Seed Germination of Lycium barbarum L. (Red Goji) Using Different Types of Cytokinins
Bentham Science Publishers ; https://doi.org/10.2174/012772574X313421240902052600
/content/journals/rafna/10.2174/012772574X313421240902052600
/content/journals/rafna/10.2174/012772574X313421240902052600
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  • Article Type:     Research Article
Keywords: in vitro germination ; kinetin ; Red goji ; chlorophyll ; 6-benzylaminopurine ; thidiazuron

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