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image of Treating Burn Infections With Topical Delivery of Positively Charged Norfloxacin-Loaded Lipid-Polymer Hybrid Nanoparticles

Abstract

Background

Norfloxacin (NFX) is a wide-spectrum antibacterial agent that suffers from low water solubility and first-pass metabolism. This diminishes its oral bioavailability by 60-70%.

Objective

This work aims to formulate a topical gel of NFX-loaded lipid polymer hybrid nanoparticles (NFX-LPHNPs) that combine the merits of liposomes and polymeric nanoparticles to overcome these problems.

Methods

NFX-LPHNPs formulations were developed using Precirol ATO (lipid) and Eudragit RL100 (polymer). They were characterized for particle size, uniformity of distribution, entrapment efficiency, zeta potential, and in-vitro release. Box–Behnken design was applied to study sequentially different variables' impact on material attributes. Then the optimized formula was re-evaluated, and incorporated in an HPMC-gel formulation. The gel formulation was evaluated for its physical properties, -release, and antibacterial activity.

Results

NFX-LPHNPs exhibited particle sizes ranging from 28.92 to 730.30 nm. Particles were uniformly distributed with a positively charged surface (indicated by zeta potential with values from +3.91 to +60.2 mV). Formulations showed a % cumulative drug release of 87.9-100% in 8 h. The optimized formula showed a satisfied fit of measured-to-predicted responses with 159 nm particle size, 92.61% release and 79.2% entrapment efficiency Gel formulation showed a sustained release over 24 h. Antibacterial testing against and revealed enhanced activity of NFX-LPHNPs against these pathogens compared to bare NFX loaded gel.

Conclusion

These results illustrated the high potential of lipid-polymer hybrid nanoparticles to improve NFX activity against resistant pathogens common in burn infections. Moreover, the topical application helps overcome Norfloxacin oral-associated problems.

© 2024 Bentham Science Publishers
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2024-12-09
2025-01-20

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Treating Burn Infections With Topical Delivery of Positively Charged Norfloxacin-Loaded Lipid-Polymer Hybrid Nanoparticles
Bentham Science Publishers ; https://doi.org/10.2174/0126673878316672241122041157
/content/journals/raddf/10.2174/0126673878316672241122041157
/content/journals/raddf/10.2174/0126673878316672241122041157
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  • Article Type:     Research Article
Keywords: norfloxacin ; eudragit RL100 ; antibacterial activity ; box–behnken design ; burn infections ; precirol ATO ; Lipid polymer hybrid nanoparticles

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