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2000
Volume 13, Issue 8
  • ISSN: 0929-8665
  • E-ISSN: 1875-5305

Abstract

We report the stepwise transformation of a linear peptide epitope recognized by the anti-transforming growth factor a monoclonal antibody Tab2 into peptomers and finally into peptoid analogs. The key experiment in this study is the substitution analysis in which each position of the peptide is exchanged by a set of different peptoid building blocks resulting in a peptidomimetic array. After probing the array toward antibody binding, the best binding peptomer spots were selected and subjected to a successive transformation. The best peptoid found in this study has a KD of 200 nM when binding to Tab2, which is only 8-fold higher than the starting peptide. Moreover, this approach permits to ask directly questions about the transformation of peptide lead structures into non-peptidic compounds in the context of protein recognition.

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/content/journals/ppl/10.2174/092986606777841299
2006-08-01
2025-06-15
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/content/journals/ppl/10.2174/092986606777841299
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  • Article Type:
    Research Article
Keyword(s): peptid-peptoid transformation; peptidomimetic; peptoid; peptomer; SPOT synthesis
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