Structure Study of Recombinant RGD-Hirudin by Vibrational and Circular Dichroism Spectroscopy

X. Liu; W. Mo; L. Dai; X. Yan; H. Song

doi:10.2174/092986606774502135

ISSN: 0929-8665
E-ISSN: 1875-5305

Structure Study of Recombinant RGD-Hirudin by Vibrational and Circular Dichroism Spectroscopy
Authors: X. Liu¹, W. Mo², L. Dai³, X. Yan⁴ and H. Song⁵
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¹ Key Laboratory of Molecular Medicine, the Ministry of Education, Fudan University, Shanghai, 200032, P.R.China. ² Key Laboratory of Molecular Medicine, the Ministry of Education, Fudan University, Shanghai, 200032, P.R.China. ³ Key Laboratory of Molecular Medicine, the Ministry of Education, Fudan University, Shanghai, 200032, P.R.China. ⁴ Key Laboratory of Molecular Medicine, the Ministry of Education, Fudan University, Shanghai, 200032, P.R.China. ⁵ Key Laboratory of Molecular Medicine, the Ministry of Education, Fudan University, Shanghai, 200032, P.R.China.
Source: Protein and Peptide Letters, Volume 13, Issue 1, Jan 2006, p. 47 - 51
DOI: https://doi.org/10.2174/092986606774502135
- Available online: 01 Jan 2006

Abstract

The secondary structure of a new type of recombinant RGD-hirudin, which has the activities of anti-thrombin and anti-platelet aggregation, has been studied by Fourier transform infrared spectroscopy (FT-IR), Raman spectroscopy and circular dichroism (CD) methods. The distribution of various secondary structure elements was determined using only a very small amount of sample protein. It was found that the recombinant RGD-hirudin contains about 26% extended chain, 21% β-turn and 53% unordered structure, leaving no α-helix. The results showed that the regular secondary structure of recombinant RGD-hirudin is increased compared with wild-type hirudin. The RGD segment that is located at the end of a long arm of a β-sheet is thought to play an important role in the additional function of anti-platelet aggregation. Throughout the experiments, FT-IR, Raman spectroscopy and CD generated mutually reinforcing results.

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2006-01-01

2025-05-23

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Article Type: Research Article

Keyword(s): CD; FT-IR; Raman spectroscopy; Recombinant RGD-hirudin; secondary structure

Structure Study of Recombinant RGD-Hirudin by Vibrational and Circular Dichroism Spectroscopy

Abstract

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