Synthesis of Stable-isotope (13C and 15N) Labeled Nucleosides and Their Applications

NMR studies of stable-isotope labeled oligonucleotides have provided useful information regarding nucleic acid structures, nucleic acid - drug binding, and nucleic acid - protein interactions. For the conformational elucidation of the sugar-phosphate backbone and / or the sugar moieties in nucleic acids, 13Clabeled oligonucleotides are very useful, and for the transformation of nucleic acid - drug binding sight are 15N-labeled oligonucleotides. The present paper has introduced the recent synthetic methods of nucleosides containing either a 13C labeled sugar moiety or a 15N-labeled exocyclic amine, and applications including our research work.