Scorpion Neurotoxin: An Analgesic Antitumor Peptide Down-Regulates VGSCs and Exhibits Local Anesthetic Potency during Sciatic Nerve Block

Sylvanus Kampo; Natacha Raissa Doudou; David Zawumya Kolbilla; Thomas Winsum Anabah

doi:10.2174/0126661217299525240407162148

ISSN: 2950-5704
E-ISSN: 2950-5712

Scorpion Neurotoxin: An Analgesic Antitumor Peptide Down-Regulates VGSCs and Exhibits Local Anesthetic Potency during Sciatic Nerve Block
Authors: Sylvanus Kampo¹, Natacha Raissa Doudou², David Zawumya Kolbilla³ and Thomas Winsum Anabah⁴
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Affiliations: ¹ Department of Anesthesia and Intensive Care, School of Medical Sciences, C. K. Tedam University of Technology and Applied Sciences, Navrango, Ghana ; ² National Radiation Protection Agency, Ministry of Scientific Research and Innovation, Yaounde, Cameroon ; ³ Department of Obstetrics and Gynecology, School of Medicine, University for Development Studies, Tamale, Ghana ; ⁴ Anesthesia Unit, Habana Medical Services, Tamale, Ghana
Source: Journal of Current Toxicology and Venomics, Volume 4, Issue 1, Jan 2024, e250424229313
DOI: https://doi.org/10.2174/0126661217299525240407162148
- Received: 16 Jan 2024
- Accepted: 20 Mar 2024
- Available online: 25 Apr 2024

Abstract

Background

Some neurotoxins inhibit nerves strongly. Saxitoxins and tetrodotoxin exhibit strong affinity for various voltage-gated sodium channels (VGSCs), including Nav 1.7, but low affinity for Nav 1.8. Emerging data suggests AGAP mediate analgesia by binding voltage-independently to sodium channel site-3.

Objective

This study examined AGAP's mechanism and analgesic efficacy during sciatic nerve block.

Methods

In rats, partial sciatic nerve ligation caused rapid and persistent mechanical hyperalgesia. Mechanical hyperalgesia after medication delivery was assessed with von Frey filaments. Western blot and immunohistochemical staining assessed VGSC expression following drug treatment.

Results

The data showed that lidocaine and AGAP increased paw withdrawal threshold compared to control rats. Compared to the control group, lidocaine or AGAP inhibited VGSCs.

Conclusion

This study suggested that AGAP inhibits VGSCs to elicit lidocaine-like analgesia.

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2024-04-25

2025-01-19

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/content/journals/jctv/10.2174/0126661217299525240407162148

Scorpion Neurotoxin: An Analgesic Antitumor Peptide Down-Regulates VGSCs and Exhibits Local Anesthetic Potency during Sciatic Nerve Block

J. Curr. Toxicol. Venomics 4, e250424229313 (2024); https://doi.org/10.2174/0126661217299525240407162148

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ARRIVE checklist is available as suypplementary material on the publisher website along with the published article.

Article Type: Research Article

Keyword(s): AGAP; immune histo chemical stain; local anesthetic; mechanical hyperalgesia; voltage-gated sodium channels; western blot

Scorpion Neurotoxin: An Analgesic Antitumor Peptide Down-Regulates VGSCs and Exhibits Local Anesthetic Potency during Sciatic Nerve Block

Abstract

From This Site

ARRIVE checklist is available as suypplementary material on the publisher website along with the published article.

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