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Volume 4, Issue 1
  • ISSN: 2950-5704
  • E-ISSN: 2950-5712

Abstract

Background

Spices, including and are not only popular for improving food flavour but are also accepted worldwide for their health benefits in various disorders.

Objective

This study aimed to elucidate the possible mechanism for the anti-apoptotic effect of terpene-rich extract of and against the rodent model of cardiotoxicity.

Methods

Effects of standardized terpenoids-rich aqueous methanolic extract of two commonly used spices, . Roxb. and Linn., were investigated on doxorubicin-induced apoptotic changes and cardiotoxicity in Wistar rats with the aim of investigating the mechanism. Prior to the experiment, the extracts were subjected to quantitative estimation of possible bioactive markers of the terpenes by employing a newly developed, optimized, and validated GC-MS method along with TLC profiling.

Results

Cardiotoxicity was evident from elevated creatinine kinase (CK-MB), lactate dehydrogenase (LDH), and malondialdehyde (MDA) levels in the toxic control group after treatment with doxorubicin (2.5 mg/kg . given twice a week for three weeks). Caspase-3, Tumor necrosis factor-α (TNF-α), and Interleukin-6 (IL-6) were also induced in animals treated with doxorubicin. Treatment with and at the doses of 100 and 200 mg/kg exhibited significant (<0.001) reversal of CK-MB, LDH, MDA, Caspase-3, TNF-α, and IL-6 levels. This protective effect was further supported by the results of DNA gel electrophoresis and histopathological observations.

Conclusion

This study supports the cardioprotective role of selected spices against doxorubicin-induced cardiotoxicity through the anti-apoptotic mechanism.

© 2024 Bentham Science Publishers
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2024-06-05
2025-01-31

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Elucidation of the Possible Molecular Mechanism for the Anti-Apoptotic Effect of Terpene-Rich Extract of Amomum subulatum and Coriandrum sativum against Rodent Model of Cardiotoxicity
J. Curr. Toxicol. Venomics 4, E050624230706 (2024); https://doi.org/10.2174/0126661217302439240513160410
/content/journals/jctv/10.2174/0126661217302439240513160410
/content/journals/jctv/10.2174/0126661217302439240513160410
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  • Article Type:     Research Article
Keyword(s): Amomum subulatum Roxb.; apoptosis; cardiovascular disease; caspase-3; Coriandrum sativum Linn.; doxorubicin
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