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Volume 4, Issue 1
  • ISSN: 2950-5704
  • E-ISSN: 2950-5712

Abstract

Background

Envenomation with scorpion results in pain at the bite site, inflammation, necrosis, and neurotoxicity. Administration of anti-scorpion antiserum prepared from immunized horses is the main therapy for envenomation. However, in some cases, anaphylactic shock related to antiserum administration limits its use. Therefore, the identification of the venom component and the development of a specific neutralizing agent is very important. Identification of linear epitopes of using a peptide-displayed phage library (Ph.D.TM -7) was the main aim of the current study.

Methods

Three rounds of biopanning were performed on immobilized immunoglobulins (IgGs) isolated from the sera of an immunized horse. The biopanning process was checked by polyclonal phage enzyme-linked immunosorbent assay (ELISA). Forty blue phage colonies were randomly selected from the third round of biopanning, amplified, DNA extracted and submitted for sequencing.

Results

Polyclonal phage ELISA results confirmed the progress of the biopanning process. The antigens involved in stimulating the horse's immune system were identified, namely sodium channel toxin, potassium channel toxin, chloride channel toxin, cell protein, venom protein, and antimicrobial peptide.

Conclusion

Identified epitopes promise further research to develop novel diagnostic or therapies against envenomation.

© 2024 Bentham Science Publishers
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/content/journals/jctv/10.2174/0126661217280650231204071837
2024-01-01
2024-11-26

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References

  1. KassiriH. KasiriN. DianatA. Species composition, sex ratio, geographical distribution, seasonal and monthly activity of scorpions and epidemiological features of scorpionism in Zarrin-dasht County, Fars Province, Southern Iran.Asian Pac. J. Trop. Dis.20155S99S10310.1016/S2222‑1808(15)60867‑2
     [Google Scholar]
  2. VatanpourH. JalaliA. G Rowan E, Rahim F. Effects of Odontobuthus doriae scorpion venom on mouse sciatic nerve.Iran. J. Pharm. Res.201312145151 24250682
     [Google Scholar]
  3. MaertensC. CuypersE. AmininasabM. JalaliA. VatanpourH. TytgatJ. Potent modulation of the voltage-gated sodium channel Nav1.7 by OD1, a toxin from the scorpion Odonthobuthus doriae.Mol. Pharmacol.200670140541410.1124/mol.106.022970 16641312
     [Google Scholar]
  4. JalaliA. BosmansF. AmininasabM. OD1, the first toxin isolated from the venom of the scorpion Odonthobuthus doriae active on voltage‐gated Na + channels.FEBS Lett.2005579194181418610.1016/j.febslet.2005.06.052 16038905
     [Google Scholar]
  5. BoyerL. DeganJ. RuhaA.M. MallieJ. ManginE. Alagón A. Safety of intravenous equine F(ab’)2: Insights following clinical trials involving 1534 recipients of scorpion antivenom.Toxicon20137638639310.1016/j.toxicon.2013.07.017 23916602
     [Google Scholar]
  6. JahdasaniR. JamnaniF.R. BehdaniM. Identification of the immunogenic epitopes of the whole venom component of the Hemiscorpius lepturus scorpion using the phage display peptide library.Toxicon2016124839310.1016/j.toxicon.2016.11.247 27845058
     [Google Scholar]
  7. Kazemi-LomedashtF. JamnaniF.R. BehdaniM. ShahbazzadehD. Linear mimotope analysis of Iranian cobra (Naja oxiana) snake venom using peptide displayed phage library.Toxin Rev.2018
     [Google Scholar]
  8. AlmaaytahA. AlbalasQ. Scorpion venom peptides with no disulfide bridges: A review.Peptides201451354510.1016/j.peptides.2013.10.021 24184590
     [Google Scholar]
  9. WagstaffS.C. LaingG.D. TheakstonR.D.G. PapaspyridisC. HarrisonR.A. Bioinformatics and multiepitope DNA immunization to design rational snake antivenom.PLoS Med.200636e18410.1371/journal.pmed.0030184 16737347
     [Google Scholar]
  10. ChenJ. LiuH. YangJ. ChouK.C. Prediction of linear B-cell epitopes using amino acid pair antigenicity scale.Amino Acids200733342342810.1007/s00726‑006‑0485‑9 17252308
     [Google Scholar]
  11. Van RegenmortelM.H.V. Immunoinformatics may lead to a reappraisal of the nature of B cell epitopes and of the feasibility of synthetic peptide vaccines.J. Mol. Recognit.200619318318710.1002/jmr.768 16680720
     [Google Scholar]
  12. PonomarenkoJ.V. BourneP.E. Antibody-protein interactions: Benchmark datasets and prediction tools evaluation.BMC Struct. Biol.2007716410.1186/1472‑6807‑7‑64 17910770
     [Google Scholar]
  13. HansD. YoungP. FairlieD. Current status of short synthetic peptides as vaccines.Med. Chem.20062662764610.2174/1573406410602060627 17105445
     [Google Scholar]
  14. ArnonR. Ben-YedidiaT. Old and new vaccine approaches.Int. Immunopharmacol.2003381195120410.1016/S1567‑5769(03)00016‑X 12860175
     [Google Scholar]
/content/journals/jctv/10.2174/0126661217280650231204071837
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Identification of Linear B-cell Epitopes of Odontobuthus doriae Scorpion Venom Using Peptide Library
J. Curr. Toxicol. Venomics 4, E26661217280650 (2024); https://doi.org/10.2174/0126661217280650231204071837
/content/journals/jctv/10.2174/0126661217280650231204071837
/content/journals/jctv/10.2174/0126661217280650231204071837
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  • Article Type:     Research Article
Keyword(s): epitope; Odontobuthus doriae; peptide; phage display; Scorpion; venom
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