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2000
Volume 16, Issue 4
  • ISSN: 1874-4710
  • E-ISSN: 1874-4729

Abstract

Background: Radiopharmaceuticals labeled with [68Ga] from positron emission tomography (PET) radionuclides are utilized in nuclear medicine for non-invasive in vivo molecular imaging. Buffer solutions for radiolabeling play an important role as choosing the right buffer for the reaction helps to obtain high yield radiopharmaceuticals. Zwitterionic organic buffer 4-(2- hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES), sodium acetate (CHCOONa), sodium bicarbonate (NaHCO) buffers are widely used for labeling of peptides with [68Ga]Cl. They can be used for peptide labelings with the acidic [68Ga]Cl precursor of triethanolammonium (TEA) buffer. The cost and toxicity of TAE buffer are relatively low. Method: For [68Ga]GaPSMA-HBED-CC and [68Ga]GaDOTA-TATE labeling, the effectiveness of TEA buffer without chemical impurities in radiolabeling reactions and QC parameters in successful labeling was investigated. Results: The method used to label [68Ga]Cl with PSMA-HBED-CC peptide in the presence of TEA buffer was successful when applied at room temperature. High purity radiosynthesis suitable for clinical use was performed to obtain DOTA-TATE peptide with the addition of 363K temperature and radical scavenger. Quality control tests with R-HPLC have shown that this method is suitable for clinical use. Conclusion: We present an alternative procedure for labeling PSMA-HBED-CC and DOTATATE peptides with [68GaCl] to obtain high radioactive doses of final radiopharmaceutical products used in nuclear medicine clinical applications. We have provided a quality-controlled final product that can be used in clinical diagnostic procedures. With the use of an alternative buffer, these methods could be adapted to semi-automatic or automated modules routinely used in nuclear medicine laboratories to label [68Ga]-based radiopharmaceuticals.

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/content/journals/crp/10.2174/1874471016666230522100024
2023-12-01
2025-05-29
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