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image of Design and Characterization of Antibacterial Peptide Nanofibrils as Components of Composites for Biomaterial Applications

Abstract

Purpose

The purpose of this study was to design and synthesize the ug46 peptide, incorporate its fibrils into composite materials, and evaluate its structural and antimicrobial properties. Another objective was to utilize spectroscopy and molecular simulation, enhanced by Machine Vision methods, to monitor the aggregation process of the ug46 peptide and assess its potential as a scaffold for an antimicrobial peptide.

Method

The structural analysis of the ug46 peptide reveals its dynamic conformational changes. Initially, the peptide exhibits a disordered structure with minimal α-helix content, but as incubation progresses, it aggregates into fibrils rich in β-sheets. This transformation was validated by CD and ThT assays, which showed decreased molar ellipticity and an increase in ThT fluorescence.

Results

Laser-induced fluorescence and molecular dynamics simulations further revealed the transition from a compact native state to extended “worm-like” filament structures, influenced by peptide concentration and temperature. TEM and AFM confirmed these changes, showing the evolution of protofibrils into mature fibrils with characteristic twists. When incorporated into chitosan-bioglass composites, these fibrils significantly enhanced antimicrobial activity against pathogens such as and .

Conclusion

Overall, ug46 peptide fibrils show promise as a multifunctional scaffold with structural and antimicrobial benefits in composite biomaterials.

© 2025 Bentham Science Publishers
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2025-02-19
2025-05-16

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Design and Characterization of Antibacterial Peptide Nanofibrils as Components of Composites for Biomaterial Applications
Bentham Science Publishers ; https://doi.org/10.2174/0113892037353453241219185311
/content/journals/cpps/10.2174/0113892037353453241219185311
/content/journals/cpps/10.2174/0113892037353453241219185311
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  • Article Type:     Research Article
Keywords: composite biomaterials ; Peptides ; fibrils ; antimicrobial properties ; biomaterial applications ; chitosan-bioglass

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