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image of In-vitro,In-silico InvestigationsRevealsPotential Cytotoxic Activity of Fermentation Metabolites from Actinomycetes Isolated from Lonar Soda Lake Against HeLa Cancer Cell Lines

Abstract

Background

Actinomycetes, Gram-positive bacteria, are recognized for producing bioactive metabolites. Lonar Soda Lake, an alkaline ecosystem, hosts diverse actinomycetes with possible anticancer activities.

Aim

To assess the cytotoxic potential of fermentation metabolites from actinomycetes isolated from Lonar Soda Lake against HeLa cancer cells employing and methods.

Objectives

Evaluate the cytotoxicity of fermentation metabolites from Lonar Lake actinomycetes on HeLa cells. Execute molecular docking to forecast metabolite connections with cancer-related proteins.

Materials and Methods

The actinomycetes were isolated from the sediment sample of Lonar Lake using a selective medium and recognized by gene sequencing. Cytotoxicity on HeLa cells was assessed using the MTT assay, in consort with oxidative stress and apoptotic markers (GSH, MDA, TNF-α, and caspase 3). Molecular docking and molecular dynamics studies evaluated metabolite binding to cancer-related proteins (Bcl-2, TNF-α, caspase 3).

Results

Fermentation metabolites of three Lonar Lake Sediment isolates (LLSD), LLSD-5, LLSD-7, and LLSD-9 showing promising cytotoxic activity against HeLa cell lines by MTT assay, also significantly modulate the oxidative stress parameters (GSH, MDA), and cell apoptotic marker (TNF-α, caspase 3). IC50 values were 82.9 µg/ml (LLSD-5), 162.3 µg/ml (LLSD-7), and 20.15 µg/ml (LLSD-9). Furthermore, molecular docking displayed robust binding affinities to cancer-related proteins, uncovering the possible mechanism of action.

Conclusion

The fermentation metabolites actinomycete isolates from Lonar Lake exhibit significant cytotoxic activity against HeLa cancer cell lines. Both and analyses support the potential of these metabolites as anticancer agents.

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2025-01-08
2025-03-13

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In-vitro,In-silico InvestigationsRevealsPotential Cytotoxic Activity of Fermentation Metabolites from Actinomycetes Isolated from Lonar Soda Lake Against HeLa Cancer Cell Lines
Bentham Science Publishers ; https://doi.org/10.2174/0113892037334392241216074545
/content/journals/cpps/10.2174/0113892037334392241216074545
/content/journals/cpps/10.2174/0113892037334392241216074545
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  • Article Type:     Research Article
Keywords: fermentation metabolites ; Actinomycetes ; cytotoxic activity ; hela cancer cell lines ; molecular docking ; lonar soda lake

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