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Volume 26, Issue 2
  • ISSN: 1389-2037
  • E-ISSN: 1875-5550

Abstract

Background

Hypogalactia and agalactia in lactating mothers are the major causes of child malnutrition, mortality, morbidity, and overall ill health. The development of such treatments requires a well-designed preclinical study with suitable laboratory animals, which needs to be improved. Thus, a suitably designed study with a laboratory animal to analyse galactagogue activity, along with an assessment of the quality and quantity of milk, is required.

Objectives

This study aimed to evaluate the potential of rabbit as an animal model for studying lactogenic activity.

Methods

The structural homology of prolactin, gene prolactin receptor, and prolactin hormone in humans, rabbit, and rat was studied using BLAST and PyMol to assess similarity in the lactogenic system. Daily and cumulative milk production and pre-treatment (control) and post-treatment (three drugs) in rabbits were recorded and evaluated by analysing protein, fat, lactose, solid non-fat, and ash values. All parameters were recorded on the 0th day and at the end of weeks 1, 2, and 3. Mammary gland histopathology was performed to evaluate the effects on mammary glands.

Results

Homology studies revealed that the sequences of the human and rabbit prolactin genes, receptors, and hormones had a high similarity index. Treatment with Domperidone, Metoclopramide, and Shatavari significantly enhanced milk production by enhancing prolactin secretion; only Shatavari increased milk nutrition. Enlargement of the tubuloalveolar ducts of the mammary glands was observed.

Conclusion

Our findings suggest that rabbits are robust, reproducible, ethically superior, and preclinically relevant animals for assessing lactogenic activity.

© 2025 Bentham Science Publishers
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2024-09-03
2025-01-22

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References

  1. HoddinottP. TappinD. WrightC. Breast feeding.BMJ.2008336764988188710.1136/bmj.39521.566296.BE18420694
     [Google Scholar]
  2. DadaltoE.C. RosaE.M. Conhecimentos sobre benefícios do aleitamento materno e desvantagens da chupeta relacionados à prática das mães ao lidar com recém-nascidos pré-termo.Rev. Paul. Pediatr.201735439940610.1590/1984‑0462/;2017;35;4;0000528977129
     [Google Scholar]
  3. DòreaJ.G. Breastfeeding is an essential complement to vaccination.Acta Paediatr.20099881244125010.1111/j.1651‑2227.2009.01345.x19594471
     [Google Scholar]
  4. HughesK. Comparative mammary gland postnatal development and tumourigenesis in the sheep, cow, cat and rabbit: Exploring the menagerie.Semin Cell Dev Biol.202111418619510.1016/j.semcdb.2020.09.010
     [Google Scholar]
  5. Segura-PérezS. RichterL. RhodesE.C. Hromi-FiedlerA. Vilar-CompteM. AdnewM. NyhanK. Pérez-EscamillaR. Risk factors for self-reported insufficient milk during the first 6 months of life: A systematic review.Matern. Child Nutr.202218S3e1335310.1111/mcn.1335335343065
     [Google Scholar]
  6. BazzanoA.N. HoferR. ThibeauS. GillispieV. JacobsM. TheallK.P. A review of herbal and pharmaceutical galactagogues for breast-feeding.Ochsner J.201616451152427999511
     [Google Scholar]
  7. Newborn Mortality2023 https://www.who.int/news-room/fact-sheets/detail/levels-and-trends-in-child-mortality-report-2021
  8. HosseinzadehH. TafaghodiM. MosaviM.J. TaghiabadiE. Effect of aqueous and ethanolic extracts of Nigella sativa seeds on milk production in rats.J. Acupunct. Meridian. Stud.2013611823
     [Google Scholar]
  9. Malnutrition2023 https://www.who.int/news-room/fact-sheets/detail/malnutrition
  10. RazmjoueiP MoghaddamSK HeydariO Investigating the effect of herbal medicine on reduction of breast engorgement in breastfeeding women: A review study.Int. J. Pediatr.202031104111047
     [Google Scholar]
  11. PenagosT.F. BedoyaJ.J.V. Ruiz-CortésZ.T. Pharmacological overview of galactogogues.Vet. Med. Int.2014201412010.1155/2014/60289425254141
     [Google Scholar]
  12. AbascalK. YarnellE. Botanical Galactagogues.Altern. Complement. Ther.200814628829410.1089/act.2008.14602
     [Google Scholar]
  13. MoiniJ.A. NasimiD.A.H. NasimiD.A.A. NasimiD.A.R. A comprehensive review of clinical studies with herbal medicine on polycystic ovary syndrome (PCOS).Daru201927286387710.1007/s40199‑019‑00312‑031741280
     [Google Scholar]
  14. PandeyR. BhairamM. ShuklaS.S. GidwaniB. Colloidal and vesicular delivery system for herbal bioactive constituents.Daru202129241543810.1007/s40199‑021‑00403‑x34327650
     [Google Scholar]
  15. DwivediJ. YadavS. Advents in contraception: immunocontraception.IJTA.20121519
     [Google Scholar]
  16. KrishnasamyS. AbellT.L. Diabetic gastroparesis: principles and current trends in management.Diabetes Ther.20189S1Suppl. 114210.1007/s13300‑018‑0454‑929934758
     [Google Scholar]
  17. SangerG.J. AndrewsP.L.R. A history of drug discovery for treatment of nausea and vomiting and the implications for future research.Front. Pharmacol.2018991310.3389/fphar.2018.0091330233361
     [Google Scholar]
  18. FerrierJ. Domperidone as an unintended antipsychotic.Can. Pharm. J.2014147276710.1177/1715163514521969
     [Google Scholar]
  19. NiceF.J. Selection and use of galactogogues. ICAN infant, child.Adolesc Nutr.20157192194
     [Google Scholar]
  20. McGuireT.M. Drugs affecting milk supply during lactation.Aust. Prescr.20184117910.18773/austprescr.2018.00229507453
     [Google Scholar]
  21. Shatavari.8th edNew DelhiMinistry of Health Family Welfare, Government of India2018
     [Google Scholar]
  22. PatelV JoshiA KalmaR ParmarS DamorS ChaudharyK. Asparagus racemosus Jivanti (Leptadenia reticulata) and Methi (Trigonella foenum-graecum) the herbal galactogogues for ruminants.201672317
     [Google Scholar]
  23. RaviR. JosephJ. Effect of fenugreek on breast milk production and weight gain among Infants in the first week of life.Clinic. Epidem. Global Health202083656-66010.1016/j.cegh.2019.12.021
     [Google Scholar]
  24. BadgujarS.B. BandivdekarA.H. Evaluation of a lactogenic activity of an aqueous extract of Cyperus rotundus Linn.J. Ethnopharmacol.2015163394210.1016/j.jep.2015.01.01925625349
     [Google Scholar]
  25. SahooH. BhaijiA. SantaniD. Lactogenic activity of Teramnus labialis (Linn.) fruit with special reference to the estimation of serum prolactin and cortisol level in nursing rats.Indian J. Pharmacol.201648671571910.4103/0253‑7613.19485628066113
     [Google Scholar]
  26. ToledoI.M. ZhaoX. LacasseP. Effects of milking frequency and domperidone injections on milk production and prolactin signaling in the mammary gland of dairy cows.J. Dairy Sci.202010321969198110.3168/jds.2019‑1733031864734
     [Google Scholar]
  27. Mustofa YulianiF.S. PurwonoS. SadewaA.H. DamayantiE. HeriyantoD.S. Polyherbal formula (ASILACT®) induces Milk production in lactating rats through Upregulation of α-Lactalbumin and aquaporin expression.BMC Comple. Med.Therap.202020136810.1186/s12906‑020‑03152‑733243201
     [Google Scholar]
  28. HussainN.H. NoorN.M. IsmailS.B. ZainuddinN.A. SulaimanZ. Metoclopramide for milk production in lactating women: A systematic review and meta-analysis.Korean J. Fam. Med.202142645346310.4082/kjfm.20.023834871486
     [Google Scholar]
  29. ChithraS. PriyaS. RaibyP.P. Evaluvation of the lactogenic activity of erythrina indica lam. leaves.Indian J. Tradit. Knowl.202221552557
     [Google Scholar]
  30. YulianiFS. PurwonoS. SadewaAH. DamayantDS. Polyherbal formula (ASILACT®) induces Milk production in lactating rats through Upregulation of α-Lactalbumin and aquaporin expression.BMC Complement Med. Ther.202020368
     [Google Scholar]
  31. ZhongJ. LiangY. ChenY. ZhangJ. ZouX. DengJ. WangD. SunY. LiM. Study and experimental validation of the functional components and mechanisms of Hemerocallis citrina baroni in the treatment of lactation deficiency.Foods2021108186310.3390/foods1008186334441640
     [Google Scholar]
  32. MirN.A. KumarP. RatherS.A. SheikhF.A. WaniS.A. Effect of supplementation of Tinospora cordifolia on lactation parameters in early lactating murrah buffaloes.Buffalo Bull.2015341720
     [Google Scholar]
  33. Sanjeev KumarR.P.S. BaghelA.K. Effect of Chandrasoor (Lepidium sativum) supplementation on dry matter intake, body weight and milk yield in lactation murrah buffaloes.Buffalo Bull.201130262266
     [Google Scholar]
  34. BudionoB. PertamiS.B. Kasiati ArifahS.N. Atho’illahM.F. Lactogenic effect of Polyscias scutellaria extract to maintain postpartum prolactin and oxytocin in lactating rats.J. Ayurveda Integr. Med.202314210058010.1016/j.jaim.2022.10058035753895
     [Google Scholar]
  35. SumanthM. NarasimharajuK. Evaluation of galactagogue activity of lactovedic: A polyherbal formulation.Int. J. Green Pharmacy.201151616410.4103/0973‑8258.82102
     [Google Scholar]
  36. LiuH. HuaY. LuoH. ShenZ. TaoX. ZhuX. An herbal galactagogue mixture increases milk production and aquaporin protein expression in the mammary glands of lactating rats.Evid. Based Complement. Alternat. Med.201520151610.1155/2015/76058526075000
     [Google Scholar]
  37. Kennethh. Official Methods of Analysis The AssociationArlington, VA1990
     [Google Scholar]
  38. AhmadzadehA. BarnesM.A. GwazdauskasF.C. AkersR.M. Dopamine antagonist alters serum cortisol and prolactin secretion in lactating Holstein cows.J. Dairy Sci.20068962051205510.3168/jds.S0022‑0302(06)72272‑X16702268
     [Google Scholar]
  39. DruryR.A. WallingtonE.A. Carleton’s Histological Technique.New YorkOxford University Press1983
     [Google Scholar]
  40. SinghB. The common integument.Dyce, Sack, Wensing’s Textb Vet Anat.Philadelphia, PA, USASaunders2016341358
     [Google Scholar]
  41. SjaastadO.V. SandO.H.K. The endocrine system. Physiol Domest Anim.Scandinavian Veterinary PressOslo, Norway2010219258
     [Google Scholar]
  42. SimelaneM.B.C. LawalO.A. DjarovaT.G. MusabayaneC.T. SinghM. OpokuA.R. Lactogenic activity of rats stimulated by Gunnera perpensa L. (Gunneraceae) from South Africa.Afr. J. Tradit. Complement. Altern. Med.20129456157310.4314/ajtcam.v9i4.1423983393
     [Google Scholar]
  43. DhillodS. KarD. PatilC.S. SahuS. SinghN. Study of the dairy characters of lactating Murrah buffaloes on the basis of body parts measurements.Vet. World2017101172110.14202/vetworld.2017.17‑2128246443
     [Google Scholar]
  44. MartignoniM. GroothuisG.M. de KanterR. Species differences between mouse, rat, dog, monkey and human CYP-mediated drug metabolism, inhibition and induction.Expert Opin. Drug Metab. Toxicol.20062687589410.1517/17425255.2.6.87517125407
     [Google Scholar]
  45. FegerosK. ZervasG. StamouliS. ApostolakiE. Nutritive value of dried citrus pulp and its effect on milk yield and milk composition of lactating ewes.J. Dairy Sci.19957851116112110.3168/jds.S0022‑0302(95)76728‑57622722
     [Google Scholar]
  46. DonovanT.J. BuchananK. Medications for increasing milk supply in mothers expressing breastmilk for their preterm hospitalised infants.Cochrane Database Syst. Rev.2012310.1002/14651858.CD005544.pub2
     [Google Scholar]
  47. ReddymasuS.C. SoykanI. McCallumR.W. Domperidone: review of pharmacology and clinical applications in gastroenterology.Am. J. Gastroenterol.200710292036204510.1111/j.1572‑0241.2007.01255.x17488253
     [Google Scholar]
  48. OsadchyA. MorettiM.E. KorenG. Effect of domperidone on insufficient lactation in puerperal women: a systematic review and meta-analysis of randomized controlled trials.Obstet. Gynecol. Int.20121710.1155/2012/64289322461793
     [Google Scholar]
  49. WallE. McFaddeT. Regulation of mammary development as it relates to changes in milk production efficiency.Milk Prod - An Up-to-Date Overv. Anim. Nutr. Manag. Heal.InTech201210.5772/50777
     [Google Scholar]
  50. SakhaK. BehbahanA.G. Training for perfect breastfeeding or metoclopramide: which one can promote lactation in nursing mothers?Breastfeed. Med.20083212012310.1089/bfm.2007.002018564001
     [Google Scholar]
  51. El-SabroutK. AggagS. El-RaffaA. Comparison of milk production and milk composition for an exotic and a local synthetic rabbit lines.Vet. World201710552652910.14202/vetworld.2017.526‑52928620257
     [Google Scholar]
  52. IngramJ. TaylorH. ChurchillC. PikeA. GreenwoodR. Metoclopramide or domperidone for increasing maternal breast milk output: a randomised controlled trial.Arch. Dis. Child. Fetal Neonatal Ed.2012974F241F24510.1136/archdischild‑2011‑30060122147287
     [Google Scholar]
  53. Campbell-YeoM.L. AllenA.C. JosephK.S. LedwidgeJ.M. CaddellK. AllenV.M. DooleyK.C. Effect of domperidone on the composition of preterm human breast milk.Pediatrics20101251e107e11410.1542/peds.2008‑344120008425
     [Google Scholar]
  54. de GezelleH. OogheW. ThieryM. DhontM. Metoclopramide and breast milk.Eur. J. Obstet. Gynecol. Reprod. Biol.1983151313610.1016/0028‑2243(83)90294‑06350073
     [Google Scholar]
  55. PatelA.B. KanitkarU.K. Asparagus racemosus willd--form bordi, as a galactogogue, in buffaloes.Indian Vet. J.19694687187215389557
     [Google Scholar]
  56. BerhaneM. SinghV.P. Effect of feeding indigenous galactopoietic feed supplements on milk production in crossbred cows.Indian J. Anim. Sci.200272609611
     [Google Scholar]
  57. SabnisPB GaitondeBB JetmalaniM Effects of alcoholic extracts of Asparagus racemosus on mammary glands of rats.Indian J. Exp. Biol.19686557
     [Google Scholar]
  58. PaësC. AymardP. DebrusseA.M. LaperruqueF. BannelierC. BébinK. DuperrayJ. GohierC. Guené-GrandE. ReboursG. GidenneT. Fortun-LamotheL. CombesS. Data set on early feed intake and growth performances of rabbits fed during the suckling period with pellets differing in diameter or compression rate using a double-choice testing design.Data Brief20202910519610.1016/j.dib.2020.10519632071976
     [Google Scholar]
  59. VentrellaD. AshkenaziN. ElmiA. AllegaertK. AniballiC. DeLiseA. DevineP.J. SmitsA. SteinerL. ForniM. Bouisset-LeonardM. BacciM.L. Animal models for in vivo lactation studies: Anatomy, physiology and milk compositions in the most used non-clinical species: A contribution from the conception project.Animals202111371410.3390/ani1103071433808012
     [Google Scholar]
  60. SevrinT. Alexandre-GouabauM.C. CastellanoB. AguesseA. OuguerramK. NgyuenP. DarmaunD. BoquienC.Y. Impact of fenugreek on milk production in rodent models of lactation challenge.Nutrients20191111257110.3390/nu1111257131653107
     [Google Scholar]
  61. SaleemM. MartinH. CoatesP. Prolactin biology and laboratory measurement: An update on physiology and current analytical issues.Clin. Biochem. Rev.201839131630072818
     [Google Scholar]
  62. FreemanM.E. KanyicskaB. LerantA. NagyG. Prolactin: structure, function, and regulation of secretion.Physiol. Rev.20008041523163110.1152/physrev.2000.80.4.152311015620
     [Google Scholar]
  63. KhoranaM WongsinP TorbunsupachaiR KanjanapattanakulW. Effect of domperidone on breast milk production in mothers of sick neonates: a randomized, double-blinded, placebo-controlled trial.Breastfeed Med.2020162455010.1089/bfm.2020.0234
     [Google Scholar]
  64. Campbell-YeoM.L. AllenA.C. JosephK.S. LedwidgeJ.M. AllenV.M. DooleyK.C. Study protocol: a double blind placebo controlled trial examining the effect of domperidone on the composition of breast milk [NCT00308334].BMC Pregnancy Childbirth2006611710.1186/1471‑2393‑6‑1716719919
     [Google Scholar]
  65. FifeS GillP HopkinsM AngelloC BoswellS NelsonKM Metoclopramide to augment lactation, does it work? A randomized trial.J Matern Fetal Neonatal Med.2011241131720
     [Google Scholar]
  66. FoongS.C. TanM.L. FoongW.C. MarascoL.A. HoJ.J. OngJ.H. Oral galactagogues (natural therapies or drugs) for increasing breast milk production in mothers of non-hospitalised term infants.Cochrane Database Syst. Rev.202055CD01150532421208
     [Google Scholar]
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Comparative Study of Lactogenic Effect and Milk Nutritional Density of Oral Galactagogue in Female Rabbit
Curr. Protein Pept. Sci. 26, 125 (2025); https://doi.org/10.2174/0113892037300581240830052536
/content/journals/cpps/10.2174/0113892037300581240830052536
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  • Article Type:     Research Article
Keyword(s): domperidone; Galactagogue; metoclopramide; milk nutrition; prolactin; rabbit; shatavari

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