Current Pharmaceutical Analysis - Volume 12, Issue 3, 2016

Profens (aryl propionic acid) are non-steroidal anti-inflammatory drugs. These are broadly used for treating swelling, arthritis and fever. This article presents the comprehensive ordinary and enantioselective analyses of the profens by liquid chromatography. Liquid-liquid extraction, solid phase extraction, solid phase micro-extraction and other sample preparation techniques have been discussed. Most widely used high-performa Read More

The development and general performance of the PVC membrane sensors for tizanidine HCl (Tz) are evaluated. The method included two ion -pair complexes of tizanidine cation and tetraphenyl borate (TPB) or phosphotungstate (PT) as electroactive sensing materials. The characteristic performance of the two sensors was evaluated according to the IUPAC guidelines, which revealed that both sensors show fast, stable an Read More

Background: A fast and reliable method for the quantification of ursodeoxycholic acid (UDCA) in hepatic function medicines was developed. Objective: To develop and validate a simple cleanup procedure based on a solid-phase extraction using an OASIS HLB cartridge for extracting ursodeoxycholic acid and its internal standard, dehydrocholic acid, from pharmaceutical formulations. Methods: Resoluble separation of Read More

Furosemide is a potent high ceiling (loop) diuretic mainly used in the treatment of edematous states associated with cardiac, renal, and hepatic failure, and the treatment of hypertension. Furosemide has been classified as a class IV drug as per the biopharmaceutical classification system (BCS). D-Glucosamine HCl was used to increase the aqueous solubility of furosemide. The objective of the present work is to improve de Read More

Background: Orlistat is commonly as a potent and long-lasting specific inhibitor of gastric and pancreatic lipase. Seven potential process-related impurities and degradation products were discovered in orlistat capsule out of which two were novel. However, no analytical methods reported in the literature could separate all the impurities presented in this work. Therefore, it is necessary to develop a simple, rapid and reliable H Read More

Background: Methods reported so far in literature, considered to be less sensitive, uneconomical and time consuming; overall run time more than 10 minutes. Objective: From economic point of view and for the purpose of routine analysis, it was decided to develop a simple, more sensitive, rapid and economical HPLC method for estimation of EM. Method: A HPLC analytical method has been developed for the quantification Read More

Background: Humans are exposed continuously to a large number of chemicals from various sources and via multiple routes. One of the most common sources is the use of cosmeceuticals like lipsticks and sunscreen which contain significant amount of heavy metals like Lead (Pb) and Cadmium (Cd). Introduction: The present study was undertaken with an objective to investigate the content of heavy metals especially P Read More

Background: In the last years, the toxicity of the waters due to the presence of antibiotics is well studied. Introduction: The Microtox test, based on the measurement of bioluminescence from the bacterium Vibrio fischeri, was applied on samples of river water spiked with several antibiotics to assess acute toxicity. Thirteen antibiotics, presenting different mechanism of action, were selected as the most widely used in anti-infect Read More

Background: Given that pharmacopoeia pyrogen tests, rabbit pyrogen test, and limulus amoebocyte lysate (LAL) test have some shortcomings, supplements for pyrogen tests are necessary. Objective: This study aimed to develop a novel monocyte-based pyrogen test. Method: The human promyelocytic leukemia cell line (HL-60), which was selected for its high sensitivity to pyrogens, such as lipopolysaccharide (LPS), zymos Read More

Introduction: Predictability of partial least squares regression (PLSR) method is improved by four multivariate signal processing modelling approaches; including genetic algorithm PLS (GAPLS), net analyte processing PLS (NAP-PLS), orthogonal signal correction PLS (OSC-PLS) and direct orthogonal signal correction PLS (DOSC-PLS). The objective of the introduced work is to establish a comparison among proposed chemo Read More

An accurate and selective high-performance liquid chromatography with ultraviolet detector (HPLC-UV) method was developed for quantification of alibendol in human plasma. The sample was prepared by one-step liquid-liquid extraction (LLE) using ethyl acetate from plasma. The chromatographic retention times of alibendol and carvedilol (the internal standard; IS) were 4.3 and 3.5 min, respectively, on a reverse Read More

A series of 16 Daphniphyllum alkaloids isolated from Daphniphyllum macropodum were investigated by electrospray ionization quadrupole time-of-flight tandem mass spectrometry (ESIQTOF- MS/MS) in positive-ion mode. Deuterium labeling experiments were carried out to identify the major product ions. Although compounds have similar structures, the profiles of their MS/MS spectra vary greatly. For compounds 1 and 2, abu Read More

Background: Tamsulosin is used for the treatment of difficult urination, a common symptom of an enlarged prostate. Objective: In the present paper, for the first time a microextraction technique was introduced for the detection and determination of tamsulosin in urine and plasma samples. Method: Procedure for extraction of tamsulosin consists of hollow fiber based liquid phase microextraction (HF-LPME) followed Read More

Background: Febuxostat is xanthin oxidase inhibitor and Diclofenac Potassium is potent prostaglandin inhibitor. A fixed dose combination of febuxostat and diclofenac potassium is used for the treatment of gout. Objective: The presented work explains the development and validation of simple accurate TLC/Densitometric method for simultaneous estimation of Febuxostat (Feb) and Diclofenac potassium (Dic) in tablet for Read More