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2000
Volume 14, Issue 3
  • ISSN: 1570-1646
  • E-ISSN: 1875-6247

Abstract

Background: Hepatic cirrhosis is associated with a variety of hemodynamic and hydrodynamic abnormalities, including renal dysfunction with salt and water retention leading to ascites and dilutional hyponatremia. Objective: In the present study, a proteomic analysis of the kidney of rats subjected to bile duct ligation (BDL) has been carried out in order to identify the proteins altered under this condition. Method: The experiments were performed at the fourth week after BDL, and the protein mixtures were separated by two-dimensional gel electrophoresis using the differential in-gel system(DIGE). An internal standard composed of equal amounts of the above 12 extracts was labeled with Cy2, and loaded onto six gels. Results: More than 4,000 proteins were labeled and numbered, and around 60 proteins were differentially expressed in the kidneys of BDL rats as compared to the controls, as analyzed by De-Cyder software. The respective spots were excised and digested with trypsin for MALDITOF/TOF peptide mass fingerprinting analysis. Some of the proteins down-regulated in the kidney of the BDL rats were Ezrin, Moesin and Regucalcin, whereas Annexin-4, Cathepsin B and Heat Shock Proteins were significantly up-regulated. Discussion: The relationship of these proteins to the systemic and renal abnormalities seen in BDL rats is discussed.

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/content/journals/cp/10.2174/1570164614666170206130033
2017-09-01
2025-07-18
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/content/journals/cp/10.2174/1570164614666170206130033
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