Synthesis of Novel α-Amino Acids Bearing 1,2,4-Triazinone and Steroidal Moieties as Enzymatic Affects (Cellobaiase Activity) – Part II

Novel α-amino acids bearing 1,2,4-triazinone and steroidal moieties (N,C-disubstituted glycine) (5a and 5b) and the related systems (6 and 7) have been produced from condensation of 6-(8-aminophenyl)-4-phenyl-3-thioxo-1,2,4-triazin(2H)-5-one (1) with the selective steroids, 5-α-Androstan-17β-ol-3-one (2a) and Transandrosterone (2b) followed by addition of hydrocyanic acid and acidic hydrolysis. A nucleophilic substitution of mercapto group by 4-fluoro aniline and sulfanilamide of 5 afforded the corresponding α-amino acids 6 and 7. These newly synthesis compounds are further evaluated for enzymatic effects against some fungi (Cellobiase activity).