Current Nutrition & Food Science - Online First

Antimicrobial resistance is a growing global concern, highlighting the urgent need for effective non-antibiotic alternatives to treat urinary tract infections. Effortlessly blending traditional Unani knowledge with modern scientific evaluation, this study highlights the potential of Unani formulations in managing uncomplicated UTIs. This study's findings underscore the importance of exploring and bridging the gap between Unani medicine and evidence-based practices. This study investigates the efficacy and safety of an Unani Pharmacopeial powder formulation and Sharbat Banafsha in treating women with uncomplicated urinary tract infections (UTI).

The pre and post-test pilot self-control clinical study involved 13 participants with uUTI who received 3.5 g powder Unani formulation comprising Lagenaria siceraria (Molina) Standl, Malva sylvestris L., Cucumis sativus L. and Cucumis melo L., Portulaca oleracea L., and Adiantum capillus L. with Sharbat Banafsha (syrup of Viola odorata L.) 25 ml twice daily for 8 days. The primary outcomes were assessed using the urinary tract infection symptoms assessment (UTISA) questionnaire, while secondary outcomes included urine culture and sensitivity, as well as health-related quality of life (HRQoL) assessment using the SF-12 health survey. The data was analyzed, and p<0.05 was considered significant.

The UTISA total score from 15.69±2.18 was reduced to 4.00±5.07 on day 9 with a significance difference(p<0.001). On day 14, 84.61% of the population had negative cultures. The total SF-12, PCS, and MCS scores showed significant differences from Day 0 to 60 (p>0.05). No adverse effects were reported in both groups. HPLC analysis confirmed the presence of the plant metabolite luteolin in both the powder and syrup.

The data explores the effectiveness of Unani formulations for bacterial urinary tract infections (UTI), showing no adverse effects while also improving the HRQoL of women. These herbal products contain luteolin, a bioactive molecule with antimicrobial properties.

CTRI/2020/02/023404 dated 17/02/2020.

Babbularishta is an Ayurvedic medicine in liquid form used for the treatment of phthisis, skin diseases, urinary disorders,, and breathing problems. It is also beneficial in cases of blood disorders, acne, dermatitis, hemoptysis, lung injury, asthma, persistent cough, whooping cough, etc.

The functional role of this study is to develop a simple, novel, precise, accurate, reliable, and selective RP-HPLC method for the estimation of piperine content in herbal drug formulations (Babbularista).

A traditional approach to method development could fail to meet the desired separation. Consideration of the current regulatory requirement for analytical method development and RP-HPLC method for routine analysis of piperine in the herbal formulation has been optimized using an analytical Quality by Design (QbD) approach. Three laboratory formulations and three marketed preparations of Babularishta were investigated for organoleptic parameters, physicochemical parameters, and validation. The mobile phase composition (methanol and water % as A), pH (B), and flow rate (mL/min as C) were selected as independent variable,. In contrast, retention time (min. as Y2), area (AU as Y1) is selected as dependent variables.

The fingerprinting method was developed and optimized by using the AQbD approach. Piperine content was found to be in Piper nigrum (Marica) 3.01±0.01527% w/w and in Piper longum (Pipali) 2.74±0.01154% w/w in Babbularista.

An attempt has been made to develop quality control parameters of Babbularista by developing a suitable and sensitive HPLC methods for the quantitation of piperine in herbal drug formulations (Babbularista). The developed method was found to be simple, accurate, precise,, and economical for the estimation of piperine in herbal formulations.

The novelty of the current research work lies in developing and optimizing the RP-HPLC method by the AQbD approach for the estimation of piperine content in herbal formulations, Babbularista.

Cyprinus carpio, as a commercial fish in Iran, has an important effect on human health and nutrition.

The aim of this study was to investigate the effects of oil sources in the diet on the nutritional value and fatty acid composition of C. carpio fish.

Soybean oil (SO), palm oil (PO), fish oil (FO), and oil (soybean and palm) (SPO) contained in the carp diet were fed.

The quality of fatty acids in the fish fillet from SO and PO was significantly better than the FO diet. Total SFA (saturated fatty acids) in carp fillet fat fed with PO was significantly higher than others (p<0.05). In carp fillets fed with experimental diets, there was a significant difference between monounsaturated fatty acids, polyunsaturated fatty acids, high unsaturated fatty acids, and n-3/n-6 fatty acids (p>0.05). The highest amounts of alpha-linolenic acid, linoleic acid, and oleic acid were observed in fish fillets with FO and SO, respectively. Although the percentage of eicosapentaenoic acid in fillets fed with FO and SPO diets was higher than in other treatments, no significant difference in docosahexaenoic acid concentration was observed between all treatments (p<0.05).

The present study showed that oil sources (SO, PO, SPO) fed by C. carpio for 56 days are suitable in terms of productivity and efficiency.