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2000
Volume 21, Issue 2
  • ISSN: 1573-4013
  • E-ISSN: 2212-3881

Abstract

Background

is an opportunistic pathogen protected from antibiotics' harmful effects by various mechanisms. One of the most important mechanisms is the activation of exudation systems. Therefore, identifying such targets in food contaminating bacteria can help us target them and eliminate bacteria resistant to several drugs, making food safe. Therefore, the present study aimed to trace and efflux pump genes in isolated from dairy products in Qom City, Iran.

Methods

300 samples of raw milk, 30 samples of pasteurized milk, and 30 samples of local cream were collected. After a 24-hour culture on nutrient agar and blood agar and performing warm staining, confirmatory biochemical tests were performed to identify bacteria. The disk method was used according to CLSI guidelines to determine the antibiotic sensitivity pattern. Then and efflux pump genes were detected using specific primers with PCR technique.

Results

117 strains of were isolated from 300 raw milk samples, and 7 strains were resistant to ciprofloxacin. Out of 117 isolates, 51 isolates (43.5%) had gene and 10 isolates (8.5%) had gene. Three isolates had both and genes. Among the strains with resistance to ciprofloxacin (7 isolates), 4 isolates did not have any of the genes. One isolate had and genes; 2 isolates were positive and negative, and positive and negative isolates were not observed.

Conclusions

Based on the results, among the studied isolates, the gene was more abundant. In addition, no significant relationship was found between the existence of and genes and ciprofloxacin resistance.

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2025-02-21
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  • Article Type:
    Research Article
Keyword(s): ciprofloxacin; dairy product; efflux pump; Mex A; Mex B; Pseudomonas aeruginosa
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