Skip to content
2000
  1. Home
  2. A-Z Publications
  3. Current Nanoscience
  4. Fast Track Articles
  5. Fast Track Article
image of Changes of Unit Cell Parameters in Lactose Saturated by Substances Technologically Processed by Dilution

Abstract

Background

Our research investigates how the structure of a lactose matrix can be altered by the specific drugs used to saturate it. In particular, we examine whether ultra-high dilutions of substances, such as antibodies, can change the crystal structure of lactose.

Methods

We employed methods including X-ray diffractometry, Raman spectroscopy, and granulometric content analysis.

Results

Our findings suggest that once saturated with highly diluted substances, lactose does indeed gain and retain changes in its crystalline structures [particularly cell parameters a, b, c, β]. These changes are most pronounced between intact and saturated lactose samples, and there are also differences observed when using different solutions for saturation.

Conclusion

The results support the idea that ultra-high dilutions can irreversibly change the structure of lactose, potentially due to differences in solution wetting and subsequent recrystallization of the lactose matrix.

© 2024 Bentham Science Publishers
Loading

Article metrics loading...

/content/journals/cnano/10.2174/0115734137309344240926051154
2024-10-07
2025-01-30

  • From This Site

    /content/journals/cnano/10.2174/0115734137309344240926051154
    dcterms_title,dcterms_subject,pub_keyword
    -contentType:Contributor -contentType:Concept -contentType:Institution
    10
    5
Loading full text...

Full text loading...

References

  1. Rosilio V. Costa M.L. Baszkin A. Wettability of drug loaded polymer matrices J. Dispers. Sci. Technol. 1998 19 6-7 821 841 10.1080/01932699808913217
    [Google Scholar]
  2. Singh P. Desai S.J. Simonelli A.P. Higuchi W.I. Role of wetting on the rate of drug release from inert matrices. J. Pharm. Sci. 1968 57 2 217 226 10.1002/jps.2600570202 5641661
    [Google Scholar]
  3. Guziewicz N.A. Massetti A.J. Perez-Ramirez B.J. Kaplan D.L. Mechanisms of monoclonal antibody stabilization and release from silk biomaterials. Biomaterials 2013 34 31 7766 7775 10.1016/j.biomaterials.2013.06.039 23859659
    [Google Scholar]
  4. Epstein O. The Spatial Homeostasis Hypothesis. Symmetry (Basel) 2018 10 4 103 10.3390/sym10040103
    [Google Scholar]
  5. Gudkov S.V. Penkov N.V. Baimler I.V. Lyakhov G.A. Pustovoy V.I. Simakin A.V. Sarimov R.M. Scherbakov I.A. Effect of mechanical shaking on the physicochemical properties of aqueous solutions. Int. J. Mol. Sci. 2020 21 21 8033 10.3390/ijms21218033 33126612
    [Google Scholar]
  6. Gudkov S.V. Baimler I.V. Uvarov O.V. Smirnova V.V. Volkov M.Y. Semenova A.A. Lisitsyn A.B. Influence of the Concentration of Fe and Cu Nanoparticles on the Dynamics of the Size Distribution of Nanoparticles. Front. Phys. (Lausanne) 2020 8 622551 10.3389/fphy.2020.622551
    [Google Scholar]
  7. Ryzhkina I.S. Murtazina L.I. Kiseleva Y.V. Konovalov A.I. Self-organization and physicochemical properties of aqueous solutions of the antibodies to interferon gamma at ultrahigh dilution. Dokl. Phys. Chem. 2015 462 1 110 114 10.1134/S0012501615050048
    [Google Scholar]
  8. Lobyshev V.I. Biological Activity of Solutions of Substances at Low and Ultra Low Concentrations. Biophysics (Oxf.) 2022 67 4 523 533 10.1134/S0006350922040145
    [Google Scholar]
  9. Lobyshev V.I. Evolution of High-Frequency Conductivity of Pure Water Samples Subjected to Mechanical Action: Effect of a Hypomagnetic Filed. Phys. Wave Phenom. 2021 29 2 98 101 10.3103/S1541308X21020084
    [Google Scholar]
  10. Penkov N.V. Temporal Dynamics of the Scattering Properties of Deionized Water. Phys. Wave Phenom. 2020 28 2 135 139 10.3103/S1541308X20020132
    [Google Scholar]
  11. Slatinskaya O.V. Pyrkov Y.N. Filatova S.A. Guryev D.A. Penkov N.V. Study of the Effect of Europium Acetate on the Intermolecular Properties of Water. Front. Phys. (Lausanne) 2021 9 641110 10.3389/fphy.2021.641110
    [Google Scholar]
  12. Floris I. Rose T. Rojas J.A.C. Appel K. Roesch C. Lejeune B. Pro-Inflammatory Cytokines at Ultra-Low Dose Exert Anti-Inflammatory Effect In Vitro: A Possible Mode of Action Involving Sub-Micron Particles? Dose Response 2020 18 4 10.1177/1559325820961723 33633511
    [Google Scholar]
  13. Floris I. Appel K. Rose T. Lejeune B. 2LARTH®, a micro-immunotherapy medicine, exerts anti-inflammatory effects in vitro and reduces TNF-α and IL-1β secretion. J. Inflamm. Res. 2018 11 397 405 10.2147/JIR.S174326 30464572
    [Google Scholar]
  14. Jacques C. Floris I. Lejeune B. Ultra-Low Dose Cytokines in Rheumatoid Arthritis, Three Birds with One Stone as the Rationale of the 2LARTH® Micro-Immunotherapy Treatment. Int. J. Mol. Sci. 2021 22 13 6717 10.3390/ijms22136717 34201546
    [Google Scholar]
  15. Malarczyk E. Pazdzioch-Czochra M. Grąz M. Kochmańska-Rdest J. Jarosz-Wilkołazka A. Nonlinear changes in the activity of the oxygen-dependent demethylase system in Rhodococcus erythropolis cells in the presence of low and very low doses of formaldehyde. Nonlinear Biomed. Phys. 2011 5 1 9 10.1186/1753‑4631‑5‑9 22104369
    [Google Scholar]
  16. Kim W.K. Kim Y.H. Hong G. Kim J.M. Han J.G. Lee J.Y. Effect of Hydrogen Nanobubbles on the Mechanical Strength and Watertightness of Cement Mixtures. Materials (Basel) 2021 14 8 1823 10.3390/ma14081823 33917068
    [Google Scholar]
  17. Kamentsev K.E. Bush A.A. The superconducting properties of YBa2Cu3Oy ceramics fabricated using ultrahigh dilution technology. Ceram. Int. 2022 48 21 32196 32204 10.1016/j.ceramint.2022.07.161
    [Google Scholar]
  18. Spitsin A.I. Bush A.A. Kamentsev K.E. Piezoelectric and dielectric properties of Bi3TiNbO9 prepared by hot pressing from powders activated using the serial dilution method. Sci. Rep. 2020 10 1 22198 10.1038/s41598‑020‑78826‑w 33335172
    [Google Scholar]
  19. Subbotin K.A. Didenko Y.S. Titov A.I. Lis D.A. Pavlov S.K. Volkov P.A. Runina K.I. Voronov V.V. Chernova E.V. Lis O.N. Kuleshova K.V. Zimina Y.I. Influence of Ultrahigh Dilution Treatment of the Charge on the Growth and Spectroscopic Properties of Nd:MgMoO4 Potential Laser Crystal. Crystals (Basel) 2024 14 1 100 https://www.mdpi.com/2073-4352/14/1/100 [Internet]. 10.3390/cryst14010100
    [Google Scholar]
  20. Carello R. Ricottini L. Miranda V. Panei P. Rocchi L. Arcieri R. Galli E. Long-term treatment with low-dose medicine in chronic childhood eczema: a double-blind two-stage randomized control trial. Ital. J. Pediatr. 2017 43 1 78 10.1186/s13052‑017‑0393‑5 28874171
    [Google Scholar]
  21. Martin-Martin L.S. Giovannangeli F. Bizzi E. Massafra U. Ballanti E. Cassol M. Migliore A. An open randomized active-controlled clinical trial with low-dose SKA cytokines versus DMARDs evaluating low disease activity maintenance in patients with rheumatoid arthritis. Drug Des. Devel. Ther. 2017 11 985 994 10.2147/DDDT.S118298 28408798
    [Google Scholar]
  22. Petrova N.V. Tarasov S.A. Epstein O.I. Dubroca C. Sulpice T. Highly Diluted Antibodies to eNOS Restore Endothelium Function in Aortic Rings From Hypertensive Rats. Dose Response 2022 20 2 10.1177/15593258221099281 35602582
    [Google Scholar]
  23. Geppe N.A. Blokhin B.M. Shamsheva O.V. Abdrakhmanova S.T. Alikhanova K.A. Myrzabekova G.T. Efficacy and Safety of Ergoferon in Children from 6 Months to 6 Years Old with Acute Respiratory Viral Infections in Contemporary Outpatient Practice: A Multicenter, Double-Blind, Placebo-Controlled Randomized Trial. Can. Respir. J. 2021 2021 1 9 10.1155/2021/5570178 34820034
    [Google Scholar]
  24. Khamitov R.F. Nikiforov V.V. Zaytsev A.A. Tragira I.N. Evaluation of the efficacy and safety of a complex antiviral drug based on antibodies in the treatment of adult patients with acute respiratory viral infection. Ter. Arkh. 2022 94 1 83 93 10.26442/00403660.2022.01.201345 36286922
    [Google Scholar]
  25. Mkrtumyan A. Ametov A. Demidova T. Volkova A. Dudinskaya E. Vertkin A. Vorobiev S. A New Approach to Overcome Insulin Resistance in Patients with Impaired Glucose Tolerance: The Results of a Multicenter, Double-Blind, Placebo-Controlled, Randomized Clinical Trial of Efficacy and Safety of Subetta. J. Clin. Med. 2022 11 5 1390 10.3390/jcm11051390 35268481
    [Google Scholar]
  26. Avdeev S.N. Vizel A.A. Abrosimov V.N. Zaicev A.A. Ignatova G.L. Khamitov R.F. Mikhaylusova M.P. Shapovalova J.S. Pavlysh E.F. Trofimov B.I. Emelyanov A.V. Martynenko T.I. Martynenko V.A. Kostina N.E. Chizhov D.A. Chizhova O.Y. Kuzubova N.A. Makova E.V. Makarova E.V. Management of Cough in Patients with Chronic Obstructive Pulmonary Disease: Results of the Multicenter Randomized Placebo-Controlled Clinical Trial. Int. J. Chron. Obstruct. Pulmon. Dis. 2021 16 1243 1253 10.2147/COPD.S292109 33981141
    [Google Scholar]
  27. Tkacheva O.N. Mkhitaryan E.A. Kolykhalov I.V. Belova A.N. Kolokolov O.V. Zaslavsky L.G. Treatment of cognitive, behavioral and mental disorders in patients with vascular dementia: results of a multicenter, randomized, double-blind, placebo-controlled clinical trial. Zh Nevrol Psikhiatr Im S S Korsakova. 2023 123 7 41 49
    [Google Scholar]
  28. Baranova A. Lykina A. Antonova D. Smolyanskaya O. Optical Properties of Crystalline Lactose Fluidized with Dilutions of Various Substances in the Terahertz Frequency Range. Pharmaceutics 2021 14 1 32 10.3390/pharmaceutics14010032 35056928
    [Google Scholar]
  29. Konar A. Sarkar T. Sukul N.C. Sukul A. Drugs in ultra-high dilution induce changes in the enthalpy associated with loss of crystallization water in lactose. Int. J. High Dilut. Res. 2021 17 1 14 10.51910/ijhdr.v17i1.905
    [Google Scholar]
  30. Mondal P. Sukul N.C. Dey A. High dilutions of two drugs induce changes in crystal water structure of lactose as revealed by thermogravimetry and differential scanning calorimetry. Indian J. Biochem. Biophys. 2020
    [Google Scholar]
  31. Kaialy W. Martin G.P. Ticehurst M.D. Royall P. Mohammad M.A. Murphy J. Nokhodchi A. Characterisation and deposition studies of recrystallised lactose from binary mixtures of ethanol/butanol for improved drug delivery from dry powder inhalers. AAPS J. 2011 13 1 30 43 10.1208/s12248‑010‑9241‑x 21057906
    [Google Scholar]
  32. Ramos R. Gaisford S. Buckton G. Calorimetric determination of amorphous content in lactose: A note on the preparation of calibration curves. Int. J. Pharm. 2005 300 1-2 13 21 10.1016/j.ijpharm.2005.04.022 16009516
    [Google Scholar]
  33. Ameh E.S. A review of basic crystallography and x-ray diffraction applications. Int. J. Adv. Manuf. Technol. 2019 105 7-8 3289 3302 10.1007/s00170‑019‑04508‑1
    [Google Scholar]
  34. Fan F. Xiang P. Zhao L. Vibrational spectra analysis of amorphous lactose in structural transformation: Water/temperature plasticization, crystal formation, and molecular mobility. Food Chem. 2021 341 Pt 1 128215 10.1016/j.foodchem.2020.128215 33032252
    [Google Scholar]
  35. M Gaffour H Bouterfas M, Bekhti N, Derrar SN, Sekkal Rahal M. Harmonic Dynamics of a-D-Lactose in the Crystalline State. J. Mol. Imaging Dyn. 2011 01 01
    [Google Scholar]
  36. Bunkin N.F. Shkirin A.V. Ninham B.W. Chirikov S.N. Chaikov L.L. Penkov N.V. Kozlov V.A. Gudkov S.V. Shaking-Induced Aggregation and Flotation in Immunoglobulin Dispersions: Differences between Water and Water–Ethanol Mixtures. ACS Omega 2020 5 24 14689 14701 10.1021/acsomega.0c01444 32596606
    [Google Scholar]
  37. Chikramane P.S. Kalita D. Suresh A.K. Kane S.G. Bellare J.R. Why extreme dilutions reach non-zero asymptotes: a nanoparticulate hypothesis based on froth flotation. Langmuir 2012 28 45 15864 15875 10.1021/la303477s 23083226
    [Google Scholar]
/content/journals/cnano/10.2174/0115734137309344240926051154
Loading
Changes of Unit Cell Parameters in Lactose Saturated by Substances Technologically Processed by Dilution
Bentham Science Publishers ; https://doi.org/10.2174/0115734137309344240926051154
/content/journals/cnano/10.2174/0115734137309344240926051154
/content/journals/cnano/10.2174/0115734137309344240926051154
Loading

Data & Media loading...


  • Article Type:     Research Article
Keywords: TPA ; drug-delivery ; technologically processed antibodies ; Crystallography ; lactose

Most Cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error
Please enter a valid_number test