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Volume 24, Issue 12
  • ISSN: 1566-5240
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Abstract

Objective

To explore a new approach for the treatment of renal interstitial fibrosis (RIF), we detected the expression of matrix metalloproteinase-9 (MMP9) and vascular endothelial growth factor (VEGF).

Methods

Twenty-four male Sprague Dawley (SD) rats were randomly divided into 2-week normal control (2NC) group, 4-week NC (4NC) group, 2-week unilateral ureteral obstruction (2UUO) group, and 4-week UUO (4UUO) group. We performed left ureteral ligation on UUO groups. Then, we sacrificed the rats of the 2NC group and 2UUO group at 2 weeks and the other groups at 4 weeks after the surgery. Immunohistochemistry and western blot were applied to detect the expression of MMP9, VEGF, fibronectin (FN), type IV collagen (Col-IV), and transforming growth factor-β1 (TGF-β1). MMP9 levels reduced after UUO surgery. Its expression was less in the 4UUO group than in the 2UUO group (<0.05). The expression of VEGF, TGF-β1, FN, and Col-IV was higher in UUO groups than in NC groups (<0.05). The expression of these indicators was higher in the 4UUO group than in the 2UUO group (<0.05).

Results

In the correlation analysis, MMP9 levels in UUO groups had a negative correlation with the expression of TGF-β1, VEGF, Col-IV, FN, and RIF index (all <0.05). In UUO groups, VEGF levels had a positive correlation with the expression of TGF-β1, Col-IV, FN, and RIF index (all <0.05).

Conclusion

In conclusion, with the aggravation of RIF lesions, MMP9 levels decreased, and VEGF levels increased. Whether there is a mutual inhibition relationship between them remains to be confirmed by further experiments.

© 2024 Bentham Science Publishers
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2024-12-01
2024-11-26

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/content/journals/cmm/10.2174/0115665240264823231101103226
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The Expression and Molecular Mechanisms of Matrix Metalloproteinase-9 and Vascular Endothelial Growth Factor in Renal Interstitial Fibrosis in Rats
Current Molecular Medicine 24, 1540 (2024); https://doi.org/10.2174/0115665240264823231101103226
/content/journals/cmm/10.2174/0115665240264823231101103226
/content/journals/cmm/10.2174/0115665240264823231101103226
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  • Article Type:     Research Article
Keyword(s): matrix metalloproteinase-9; Renal interstitial fibrosis; transforming growth factor-β1; vascular endothelial growth factor

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