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Volume 24, Issue 11
  • ISSN: 1566-5240
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Abstract

Background

Airway remodeling is one of the reasons for severe steroid-resistant asthma related to HMGB1/RAGE signaling or Th17 immunity.

Objective

Our study aims to investigate the relationship between the HMGB1/RAGE signaling and the Th17/IL-17 signaling in epithelial-mesenchymal transformation (EMT) of airway remodeling.

Methods

CD4+ T lymphocytes were collected from C57 mice. CD4+ T cell and Th17 cell ratio was analyzed by flow cytometry. IL-17 level was detected by ELISA. The E-cadherin and α-SMA were analyzed by RT-qPCR and immunohistochemistry. The E-cadherin, α-SMA, and p-Smad3 expression were analyzed by western blot.

Results

The HMGB1/RAGE signaling promoted the differentiation and maturation of Th17 cells in a dose-dependent manner . The HMGB1/RAGE signaling also promoted the occurrence of bronchial EMT. The EMT of bronchial epithelial cells was promoted by Th17/IL-17 and the HMGB1 treatment in a synergic manner. Silencing of RAGE reduced the signaling transduction of HMGB1 and progression of bronchial EMT.

Conclusion

HMGB1/RAGE signaling synergistically enhanced TGF-β1-induced bronchial EMT by promoting the differentiation of Th17 cells and the secretion of IL-17.

© 2024 Bentham Science Publishers
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2023-10-27
2024-10-12

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/content/journals/cmm/10.2174/0115665240249953231024060610
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HMGB1/RAGE Signaling Regulates Th17/IL-17 and Its Role in Bronchial Epithelial-Mesenchymal Transformation
Current Molecular Medicine 24, 1401 (2024); https://doi.org/10.2174/0115665240249953231024060610
/content/journals/cmm/10.2174/0115665240249953231024060610
/content/journals/cmm/10.2174/0115665240249953231024060610
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  • Article Type: Research Article
Keyword(s): airway remodeling; bronchial asthma; EMT; HMGB1; RAGE; Th17/IL-17

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