Skip to content
2000
  1. Home
  2. A-Z Publications
  3. Current Molecular Medicine
  4. Volume 24, Issue 11
  5. Article
Volume 24, Issue 11
  • ISSN: 1566-5240
  • E-ISSN:

Abstract

Background

The purpose of this study was to investigate the role of miR-148b in liver injury in rats with traumatic hemorrhagic shock (THS) and to elucidate its potential mechanism.

Methods

The levels of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) in the serum of rats were detected by enzyme-linked immune sorbent assay (ELISA), and the injury of rat liver was analyzed by hematoxylin-eosin (H&E) staining. Apoptosis of rat hepatocytes and normal rat liver cell line (BRL3A) was identified by terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end labeling (TUNEL) assay and flow cytometry, respectively. MiR-148b and sirtuin 6 (SIRT6) expression was measured by quantitative reverse transcription polymerase chain reaction (qRT-PCR) and Western blot. Lactate dehydrogenase (LDH) content and cell viability were measured by commercial kits and cell counting kit-8 (CCK-8) assay, respectively. The binding sites of miR-148b and SIRT6 were predicted by the Starbase database and verified by dual luciferase reporter assay.

Results

MiR-148b expression in THS rats or ischemia-reperfusion (I/R)-treated cells was higher than in the control group. Overexpression of miR-148b further promoted the effects of I/R, which enhanced the levels of ALT, AST and LDH, cell apoptosis of liver tissue or BRL3A cells and decreased the expression of SITR6. Besides, miR-148b negatively correlated with SIRT6, and upregulated the expression of SIRT6 could partly reverse the effect of miR-148b.

Conclusion

Hepatocyte injury induced by I/R was achieved by regulating miR-148b /SIRT6 axis.

© 2024 Bentham Science Publishers
Loading

Article metrics loading...

/content/journals/cmm/10.2174/1566524023666230816112629
2023-10-19
2024-11-29

  • From This Site

    /content/journals/cmm/10.2174/1566524023666230816112629
    dcterms_title,dcterms_subject,pub_keyword
    -contentType:Contributor -contentType:Concept -contentType:Institution
    10
    5
Loading full text...

Full text loading...

References

  1. BellamyR.F. The causes of death in conventional land warfare: Implications for combat casualty care research.Mil. Med.198414925562 6427656
     [Google Scholar]
  2. LiuL. TianK. Small doses of arginine vasopressin in combination with norepinephrine "buy" time for definitive treatment for uncontrolled hemorrhagic shock in rats.Shock2013405398406 24089002
     [Google Scholar]
  3. LeiY. PengX. LiuL. DongZ. LiT. Beneficial effect of cyclosporine A on traumatic hemorrhagic shock.J. Surg. Res.20151952529540 25752214
     [Google Scholar]
  4. YangG. PengX. 4-phenylbutyrate benefits traumatic hemorrhagic shock in rats by attenuating oxidative stress, not by attenuating endoplasmic reticulum stress.Crit. Care Med.2016447e477e491 26646458
     [Google Scholar]
  5. TaniguchiT.A-O. FujimotoY. YawataH. HoriguchiM. AnB. TakegamiT. Renal venous congestion following hemorrhagic shock due to traumatic liver injury.CEN Case Rep.202110217818310.1007/s13730‑020‑00540‑3 33038002
     [Google Scholar]
  6. LiuH. XuefeiX. ChuanzhengS. Systemic inflammation and multiple organ injury in traumatic hemorrhagic shock.Front. Biosci.201520692793310.2741/4347 25961533
     [Google Scholar]
  7. GuM WangJ WangY XuY ZhangY WuW MiR-147b inhibits cell viability and promotes apoptosis of rat H9c2 cardiomyocytes via down-regulating KLF13 expression.Acta Biochim Biophys Sin20185032889710.1093/abbs/gmx144.29377979
     [Google Scholar]
  8. ZhangJ.A-O. XuY. LiuH. PanZ. MicroRNAs in ovarian follicular atresia and granulosa cell apoptosis.Reprod. Biol. Endocrinol.20191719
     [Google Scholar]
  9. HuangX. LeQ.T. GiacciaA.J. MiR-210 - micromanager of the hypoxia pathway.Trends Mol. Med.201016523023710.1016/j.molmed.2010.03.004 20434954
     [Google Scholar]
  10. PuisségurM-P. MazureN.M. BerteroT. miR-210 is overexpressed in late stages of lung cancer and mediates mitochondrial alterations associated with modulation of HIF-1 activity.Cell Death Differ.201118346547810.1038/cdd.2010.119 20885442
     [Google Scholar]
  11. JianminL. ChanyuanP. ChaoT. WenwenT. HuiL. JingG. The reaction pathway of miR-30c-5p activates lipopolysaccharide promoting the course of traumatic and hemorrhagic shock acute lung injury.Biomed Res. Int.202220223330552
     [Google Scholar]
  12. YanL. XiaoyongP. YiH. MingyingX. TaoL. LiangmingL. The calcilytic drug Calhex-231 ameliorates vascular hyporesponsiveness in traumatic hemorrhagic shock by inhibiting oxidative stress and miR-208a-Mediated mitochondrial fission.Oxid. Med. Cell. Longev.202020202413278510.1155/2020/4132785 33343806
     [Google Scholar]
  13. VyasH.S. UpadhyayK.K. DevkarR.V. miRNAs Signatures in patients with acute liver injury: Clinical concerns and correlations.Curr. Mol. Med.2020205325335
     [Google Scholar]
  14. KlieserE. MayrC. KiesslichT. WissniowskiT. FazioP.D. NeureiterD. The crosstalk of miRNA and oxidative stress in the liver: From physiology to pathology and clinical implications.Int. J. Mol. Sci.20192021526610.3390/ijms20215266
     [Google Scholar]
  15. WangX. HeY. MackowiakB. GaoB. MicroRNAs as regulators, biomarkers and therapeutic targets in liver diseases.Gut202170478479510.1136/gutjnl‑2020‑322526 33127832
     [Google Scholar]
  16. YangM.A-O. KongD.Y. ChenJ.C. Inhibition of miR-148b ameliorates myocardial ischemia/reperfusion injury via regulation of Wnt/β-catenin signaling pathway.J. Cell. Physiol.201923410177571776610.1002/jcp.28401 30820984
     [Google Scholar]
  17. PanW.M. WangH. ZhangX.F. miR-210 participates in hepatic ischemia reperfusion injury by forming a negative feedback loop with SMAD4.Hepatology20207262134214810.1002/hep.31221 32155285
     [Google Scholar]
  18. ZhangS JiangS WangH DiW DengC JinZ SIRT6 protects against hepatic ischemia/reperfusion injury by inhibiting apoptosis and autophagy related cell death.Free Radic Biol Med2018115183010.1016/j.freeradbiomed.2017.11.005.29129519
     [Google Scholar]
  19. OwattanapanichN ChittawatanaratK BenyakornT SirikunJ Risks and benefits of hypotensive resuscitation in patients with traumatic hemorrhagic shock: A meta-analysis.Scand J Trauma Resusc Emerg Med2010826110710.1186/s13049‑018‑0572‑4.30558650
     [Google Scholar]
  20. TranA YatesJ Permissive hypotension versus conventional resuscitation strategies in adult trauma patients with hemorrhagic shock: A systematic review and meta-analysis of randomized controlled trials.J Trauma Acute Care Surg201884580280810.1097/TA.0000000000001816.29370058
     [Google Scholar]
  21. HutchingsS.D. NaumannD.N. HopkinsP. MellisC. RiozziP. SartiniS. Microcirculatory impairment is associated with multiple organ dysfunction following traumatic hemorrhagic shock: The MICROSHOCK study.Crit. Care Med.2018469e889e896
     [Google Scholar]
  22. Chico-FernándezM. Barea-MendozaJ.A. Pérez-BárcenaJ. García-SáezI. Quintana-DíazM. MarinaL. Concomitant traumatic brain injury and hemorrhagic shock: Outcomes using the spanish trauma icu registry (RETRAUCI).Am. Surg.2021873370375
     [Google Scholar]
  23. TachonG. HarroisA. Microcirculatory alterations in traumatic hemorrhagic shock.Crit. Care Med.201442614331441
     [Google Scholar]
  24. KhattabH. FouadA. HamzaM. MoheyM.A. El-AkelW. GhoneimH. Relation of ALT and AST levels to the histopathological changes in liver biopsies of patients with chronic hepatitis C genotype 4.Arab J. Gastroenterol.20151625053
     [Google Scholar]
  25. XiongX. RenY. CuiY. LiR. WangC. ZhangY. Obeticholic acid protects mice against lipopolysaccharide-induced liver injury and inflammation.Biomed. Pharmacother.20179612921298
     [Google Scholar]
  26. MylonasA.I. OrfanosN.F. KarmaniolouI.I. LolisE.D. StergiouE.P. PapaloisA.E. The effects of hemorrhagic shock secondary to hepatectomy in a swine model.J. Surg. Res.20151951228234
     [Google Scholar]
  27. SaidiR.F. KenariS.K. Liver ischemia/reperfusion injury: An overview.J. Invest. Surg.2014276366379
     [Google Scholar]
  28. BaikS.W. ParkB.S. KimY.H. KimY.D. KimC.H. YoonJ.Y. Effects of remifentanil preconditioning on osteoblasts under hypoxia-reoxygenation condition.Int. J. Med. Sci.2015127583589
     [Google Scholar]
  29. ZhangL. LiC. CaoL. LiH. ZouH. LiH. microRNA-877 inhibits malignant progression of colorectal cancer by directly targeting MTDH and regulating the PTEN/Akt pathway.Cancer Manag. Res.20191127692781
     [Google Scholar]
  30. ZhuJ. ZhangC. QingY. Genistein induces apoptosis by stabilizing intracellular p53 protein through an APE1-mediated pathway.Free Radic. Biol. Med.20158620921810.1016/j.freeradbiomed.2015.05.030 26032169
     [Google Scholar]
  31. LiuQ. XuY. WeiS. GaoW. ChenL. ZhouT. miRNA-148b suppresses hepatic cancer stem cell by targeting neuropilin-1.Biosci. Rep.2015354e0022910.1042/BSR20150084
     [Google Scholar]
  32. ZhuY. NiT. LinJ. ZhangC. ZhengL. LuoM. Long non-coding RNA H19, a negative regulator of microRNA-148b-3p, participates in hypoxia stress in human hepatic sinusoidal endothelial cells via NOX4 and eNOS/NO signaling.Biochimie2019163128136
     [Google Scholar]
  33. YaoL. ChenH. WuQ. XieK. Hydrogen-rich saline alleviates inflammation and apoptosis in myocardial I/R injury via PINK-mediated autophagy.Int. J. Mol. Med.201944310481062
     [Google Scholar]
  34. AnY. WangB. WangX. DongG. JiaJ. YangQ. SIRT1 inhibits chemoresistance and cancer stemness of gastric cancer by initiating an AMPK/FOXO3 positive feedback loop.Cell Death Dis.202011211510.1038/s41419‑020‑2308‑4 32051395
     [Google Scholar]
  35. LiM ZhangZ LiuB ChenL WangM. LncRNA GAS5 upregulates miR-214 through methylation to participate in cell apoptosis of sepsis.Arch Physiol Biochem2022128512596410.1080/13813455.2020.1764051.32551941
     [Google Scholar]
/content/journals/cmm/10.2174/1566524023666230816112629
Loading
MiR-148b Caused Liver Injury in Rats with Traumatic Hemorrhagic Shock by Inhibiting SIRT6 Expression
Current Molecular Medicine 24, 1390 (2024); https://doi.org/10.2174/1566524023666230816112629
/content/journals/cmm/10.2174/1566524023666230816112629
/content/journals/cmm/10.2174/1566524023666230816112629
Loading

Data & Media loading...


  • Article Type:     Research Article
Keyword(s): alanine aminotransferase; apoptosis; I/R; liver injury; miR-148b; SIRT6; traumatic hemorrhagic shock

Most Cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error
Please enter a valid_number test