Current Metabolomics - Current Issue

The establishment of metabolomics has benefited from recent developments in analytical platforms and bioinformatics, which allow for comprehensive analysis of volatile metabolites in a biosystem. Consequently, metabolomics has become one of the most dynamic of all the omics over the last decade. Mass spectrometry based metabolomics of fungal and plant volatile metabolites has been a recent focus of intens Read More

The use of genome-scale metabolic modelling in system strategies for the production of platform chemical such as succinic acid in Escherichia coli has received renewed attention in recent years. The advances in high-throughput technologies and the development of E. coli genome-scale metabolic models (GEMs) have been considered indispensable in the current emerging strategies for strain design and biobased succinic Read More

Increasingly sophisticated instrumentation for chemical separations and identification has facilitated rapid advancements in our understanding of the metabolome. Since many analyses are performed using either mass spectroscopy (MS) or nuclear magnetic resonance (NMR) spectroscopy, the spin ½ stable 13C isotope is now widely used as a metabolic tracer. There is a strong interest in quantitative analysis of metabolic flu Read More

Background: Natural product research is the most enormous field of research in terms of the amount of data and importance of information. Natural products discovery and metabolomics deals with a crucial mode of representation of the profile of biologically active metabolites. In this regard, the profiling of the chemical makeup of complex natural plant extracts necessarily requires employing sophisticated and advanc Read More

Background: Succinic acid is an important platform and/or commodity or specialty chemical with a broad range of applications. The metabolic role of pyruvate formate lyase A (pflA) in relation to succinate production in Escherichia coli under anaerobic conditions from glycerol substrate remained largely unspecified. Methods: Herein, we identified pflA gene for the first time, as a novel gene knockout target for increasing Read More

Background: Chinese hamster ovary (CHO) cells are extremely important host cells for recombinant DNA technology with their utility requiring optimization of growth. The ability to test conditions using in silico models of growing CHO cells can help advance the optimization of bioreactor conditions towards higher viable cell concentration and increased mAb production. Methods: A new kinetic model of CHO cell metabolis Read More

Introduction: Prostate Cancer (PCa) is the second most common neoplasia in men. Because it is often diagnosed at a late stage, mortality rates remain high. Studying cancer metabolome, which reflects early changes that occur in cells, has gained relevance and may contribute to the identification of early diagnostic biomarkers and understanding tumor biology. Methods: Fourier-transform infrared (FTIR) spectroscopy i Read More