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- Volume 6, Issue 1, 2018
Current Metabolomics - Volume 6, Issue 1, 2018
Volume 6, Issue 1, 2018
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Autofluorescence of Breast Cancer Proteins
Background: The intensity of the blood as well as tissue autofluorescence (fluorescence of endogenous fluorophores) shows current structure of protein mixture, its current conformation (native or denatured state), concentration or activity depending on the external and internal conditions. Nicotine amide adenine dinucleotide (NAD+) is a cofactor in redox reactions of glycolysis and the citric acid cycle in eukaryotic cells and Read More
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Expression Activity of Selected Proangiogenic Factors in Patients with Limb Ischemia
Background: Blood plasma/serum is a characteristic mixture of naturally occurring endogenous fluorophores which are sensitive to endogenous and exogenous stress during physiological as well as pathological processes in the body. Methods: The structure of the patient's blood plasma/serum surfaces with critical limb ischemia and healthy subjects were studied and compared using methods of synchronous fluor Read More
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FTIR Spectroscopy to Study Bioeffects of Static Magnetic Fields on Neuronal-like Cell Cultures
Background: The use of Static Magnetic Fields (SMFs) in medicine, industry, and new technologies has significantly increased in the past few years. However, the potential health risks concerning the exposure to static magnetic fields remain a topic of debate. Objective: The objective of this study was to determine the influence of moderate static magnetic fields (SMFs) on human SH-SY5Y neuronal-like cells. Methods: SH-SY5 Read More
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The Dynamic Progress of Myocardial Infarction Studied by Spectroscopic Techniques
Background: Blood serum is characteristic mixture of naturally occurring endogenous fluorophores which are sensitive to endogenous and exogenous stress during physiological as well as pathological processes in the body. Methods: Dynamic changes of blood serum in patients with positive troponin and creatine kinase during myocardial infarction compared to control blood serum of healthy subjects were studied by Read More
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FTIR Spectroscopy Analysis can Highlight Induced Damage in Neuronallike Cells and Bio-protective Effectiveness of Agmatine
Background: Agmatine, an endogenous amine, is cosidered a novel neuromodulator with neuroprotective properties. However, the mechanisms involved in these protective effects are poorly understood. Methods: Fourier Transform Infrared (FTIR) spectroscopy analysis detects biomolecular changes in disordered cells and tissues. In the present study, we employ FTIR spectroscopy to characterize the changes in rotenone-ind Read More
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Cost Effective Natural Microspheres for the Removal of Pb from Wastewater
Background: Controlling aquatic pollution with natural resources is a worldwide hot topic of research, according to its positive environmental impact. Methods: Chitosan is cross linked with sodium Tripolyphosphate (TPP) to form microsphere. Then microsphere could be effectively utilized to remove Pb ion from water with a removal rate of 59.2% in 90 minutes. The microsphere is further enhanced as dried water hyacinth i Read More
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Polymeric Systems Under External Thermal Stress Studied by FTIR Technique
Authors: M.T. Caccamo, E. Calabro, S. Coppolino and S. MagazuBackground: Temperature behavior of a class of PolyEthylene Oxide and Ethylene Glycol (EG) by means of InfraRed spectroscopy as a function of temperature and concentration. Methods: EG and PEO with Mw of 1000 were purchased from Aldrich-Chemie; To collect the FTIR spectra in the 39°C-80°C temperature range by means of a FTIR Vertex 70V spectrometer by Bruker Optics. Spectra were obtained using an average Read More
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On a Queueing Theory Method to Simulate In-Silico Metabolic Networks
Authors: Emalie J. Clement, Beata J. Wysocki, Paul H. Davis and Tadeusz A. WysockiBackground: The vast catalog of metabolites and extensive set of interactions within even the simplest cells can present a daunting task for analyzing, and especially simulating, metabolic networks. Yet many metabolites have associations with functionally related processes, convenient for organizing metabolites into pathways. Any perturbation in concentration of a single metabolite involves dynamic changes in concentr Read More
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Metabonomic Evaluation of Fecal Water Preparation Methods: The Effects of Ultracentrifugation
Authors: Sandi Yen, Erin Bolte, Marc Aucoin and Emma Allen-VercoeBackground: Fecal water provides information on the state of the gut microbiota and host physiology and is a common biofluid to study, particularly for metabonomic purposes. Despite the interest in fecal water, a method of preparation has yet to be standardized. Current methods of preparing fecal water usually involve an extraction protocol combined with ultracentrifugation and filtration steps. If metabonomic analysis is the fi Read More
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Metabolic Responses of Thymus vulgaris to Water Deficit Stress
Authors: Parviz Moradi, Brian Ford-Lloyd and Jeremy PritchardBackground: During plant life, there are several factors affecting plant growth, development and finally their productivity. Water is one of the most important environmental factors, as it is the major constituent of all living organisms. This stress influences plant metabolism both directly and indirectly. Thymus vulgaris or common thyme is well known since ancient times for its medicinal and culinary uses. Its extract has antise Read More
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Plasma Metabolome Signature in Patients with Early-stage Parkinson Disease
Background: Recent decades have been marked by advances in omics sciences based on high-throughput technologies, which have enabled the measurement of enormous numbers of molecules in biosamples. In metabolomics, a large number of small molecules (metabolome) can be detected in a single run. The goal of this study was to evaluate the capacity for metabolomic analysis of blood plasma for early-stage Pa Read More
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