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Volume 25, Issue 4
  • ISSN: 1389-2002
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Abstract

Objective

5-Methoxy-α-Methyltryptamine (5-MeO-AMT) is a new psychoactive substance which is abused due to its hallucinogenic and euphoric effects. This study aimed to study the metabolic characteristics of 5-MeO-AMT.

Methods

Five rats were given intraperitoneal injection at a dose of 50 mg/kg of 5-MeO-AMT, and their urine was subsequently collected at different times within 7 days. Ultra-high performance liquid chromatographytandem high-resolution mass spectrometry (UPLC-LTQ-Orbitrap) was used to detect the precise molecular weight and fragment ions of 5-MeO-AMT and its possible metabolites in the urine sample extracted with benzene-ethyl acetate.

Results

Three metabolites, including OH-5-MeO-AMT, α-Me-5-HT, and N-Acetyl-5-MeO-AMT were identified in rats’ urine. The major metabolic pathways involved O-demethylation, hydroxylation of indole ring, and Acetylation on aliphatic amines.

Conclusion

The results of this study are an important reference for the identification and screening of toxicants of 5-MeO-AMT.

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2024-05-01
2024-11-26

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/content/journals/cdm/10.2174/0113892002295551240628061732
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UPLC-LTQ-Orbitrap Study on Rat Urinary Metabolites of 5-Methoxy-Alpha-Methyltryptamine
Current Drug Metabolism 25, 298 (2024); https://doi.org/10.2174/0113892002295551240628061732
/content/journals/cdm/10.2174/0113892002295551240628061732
/content/journals/cdm/10.2174/0113892002295551240628061732
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  • Article Type:     Research Article
Keyword(s): 5-MeO-AMT; hallucinations; high resolution; metabolic pathway; metabolites; UPLC-LTQ-Orbitrap

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