Combinatorial Chemistry & High Throughput Screening - Volume 7, Issue 1, 2004

As 2003 begins, Combinatorial Chemistry & High Throughput Screening has completed its six year of publication. During the last year, several new members joined the Editorial Board replacing others whose years of service were much appreciated. In particular, I would like to acknowledge the addition of the following new members of our Editorial Board: Dr. Brian Kay, Argonne National Laboratories, Argonne, IL. Brian has alrea Read More

Drugs exert their functions mainly by affecting proteins. Therefore, it seems straightforward to focus on proteins in order to investigate drug effects. Unfortunately, proteins are of very high complexity, rendering it much more difficult to screen for protein alterations as compared to gene regulation. However, the efficiencyand applicability of proteome analysis has been dramatically increased recently. We are on the way t Read More

Screening a library of molecular graphs for an exact or approximate match with one particular molecular graph, the query graph, is reduced to list comparisons. The lists contain lengths of shortest paths ingraph Voronoi regions. This induces the notion of shortest path similarity. All graphs that are shortest path similar to the query graph are efficiently retrievable. The same applies to approximate or similarity matching. For the r Read More

We introduce a graphical representation of DNA primary sequences by taking four special vectors in a 3-D space to represent the four nucleic acid bases in DNA sequences, so that a DNA primary sequence isdenoted in a 3-D space by a successive vector sequence which is a directed walk on the space. It is demonstrated that this representation has no overlap and intersection and allows numericalcharacterization.

DNA analysis plays a great role in genetic and medical research, and clinical diagnosis of inherited diseases and particular cancers. Development of new methods for high throughput DNA analysis is necessitated with incoming of post human genome era. A new powerful analytical technology, called microchip capillary electrophoresis (MCE), can be integrated with some experimental units and is characterized by high-speed, s Read More

Time-resolved fluorescence (TRF) assay formats are frequently used technologies in highthroughput screening. In this article, we have characterised the novel Plate::Vision2 96-microlens array reader (Carl Zeiss Jena GmbH, Germany) and compared it to the novel LEADseeker Generation IV multimodality imaging system (LEADseeker Gen IV;; Amersham Biosciences UK Ltd., UK) for applications in the TRF mode. Read More

When using multiple targets and libraries, selection of affinity reagents from phage-displayed libraries is a relatively time-consuming process. Herein, we describe an automation-amenable approach to accelerate the process by using alkaline phosphatase (AP) fusion proteins in place of the phage ELISA screening and subsequent confirmation steps with purified protein. After two or three rounds of affinity selection, the open Read More

In this paper we introduce a quantitative model that relates chemical structural similarity to biological activity, and in particular to the activity of lead series of compounds in high-throughput assays. From this model we derive the optimal screening collection make up for a given fixed size of screening collection, and identify the conditions under which a diverse collection of compounds or a collection focusing on particular reg Read More