Combinatorial Chemistry & High Throughput Screening - Volume 13, Issue 7, 2010

Work in Analytical Chemistry is divided into two interrelated parts: The methodological part includes the study of physicochemical processes from the analytical point of view, construction of the related instrumentation and the development and optimization of analytical methods. The practical part adjusts these analytical methods to the necessities of the users. Analytical methods widely utilize the knowledge of all the other disc Read More

The electrochemical behavior of triflusal (TRF) and aspirin (ASA), before and after hydrolysis in water and in alkaline medium using two different electrode surfaces, glassy carbon and boron doped diamond, was studied by differential pulse voltammetry over a wide pH range. The hydrolysis products were 2-(hydroxyl)-4-(trifluoromethyl)- benzoic acid (HTB) for triflusal and salicylic acid (SA) for aspirin, which in vivo represent t Read More

A sensitive aptamer based electrochemical biosensor to detect human immunoglobulin E (IgE) is presented in this study. 5' Biotin labeled 45 mer DNA aptamer sequence was immobilized onto streptavidin coated graphite surfaces. Interaction between human IgE and DNA aptamer was monitored by Electrochemical Impedance Spectrometry (EIS) in a 0.48nM detection limit of IgE. EIS analyses are based on electron tran Read More

The interaction of anticancer drug irinotecan (CPT-11), which is the inhibitor of the Topoisomerase I enzyme, with fish sperm double stranded deoxyribonucleic acid (dsDNA) and synthetic short oligonucleotides were studied electrochemically based on the oxidation signals of guanine and CPT-11 by using differential pulse voltammetry (DPV) and cyclic voltammetry (CV) at pencil graphite electrode (PGE). In this work, three Read More

A rapid electrochemical procedure for the determination of dipyrone was successfully developed at a carbon nanotube modified graphite-epoxy resin composite (GrEC) electrode. The composite electrode was used as support on which multi-walled carbon nanotubes (MWCNT) were immobilised by 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide together with N-hydroxysuccinimide (EDC-NHS) in a chitosan (Chit) matri Read More

A simple, reliable and selective differential pulse (DP) and square wave (SW) voltammetric methods at glassy carbon (GC) and boron-doped diamond (BDD) electrodes of lornoxicam in pharmaceutical dosage form and in spiked human serum samples have been developed and evaluated. The possible oxidation mechanism was discussed. The voltammetric study of the model compounds allowed elucidating the possible oxi Read More

A rapid and sensitive voltammetric sensor based on reduction of betamethasone has been developed using single wall carbon nanotube modified edge plane pyrolytic graphite electrode (SWNT/EPPGE). The reduction of betamethasone occurred in a well-defined, pH dependent peak. Linear calibration curve was obtained in the range 1 to 25 nM in 1.0 M phosphate buffer solution (PBS) of pH 7.2 with the limit of detection (3σ/s Read More

Electrochemical techniques provide valuable information about drug molecules and their mechanisms in the body such as metabolism, which is one of the important actions in drug discovery. Since, many physiological processes depend on oxido-reduction reactions, it is not difficult to find associations between electrochemical and biological reactions regarding electron transfers. To investigate this proposal two compounds Read More

In recent years increased attention has been focused on the ways in which drugs interact with DNA, with the goal of understanding the toxic as well as chemotherapeutic effects of many molecules. The development of fast and accurate methods of DNA damage detection is important, especially caused by anticancer drugs or hazard compounds. The DNA-electrochemical biosensor is a very good model for evaluation of nucleic ac Read More

Aptamers are single stranded DNA or RNA ligands which can be selected for different targets starting from a huge library of molecules containing randomly created sequences. Aptamers have been selected to bind very different targets, from proteins to small organic dyes. In the last years great progress has been accomplished in the development of aptamer-based bioanalytical assays with different detection techniques. T Read More

The measurement of antiepileptic drugs (AEDs) in different samples has received considerable attention due to the direct correlation between clinical effects and plasma concentration. Numerous methods have been extensively applied to the analysis of AEDs since many years ago providing reliable and accurate results. This paper provides an overview of electrochemical techniques used for the analysis of different Read More

Recently, the use of electrochemistry and combination of this method with spectroscopic and other analytical techniques are becoming one of the important approaches in drug discovery and research as well as quality control, drug stability, determination of physiological activity, and measurement of neurotransmitters. Many fundamental physiological processes depend on oxido-reduction reactions in the body. Theref Read More