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Volume 28, Issue 4
  • ISSN: 1386-2073
  • E-ISSN: 1875-5402

Abstract

Background

Previous studies have found that matrine (MAT) effectively treated Ulcerative Colitis (UC). The purpose of this study is to explore its mechanism based on the HMGB1/NLRP3/Caspase-1 signaling pathway.

Methods

MAT was administered intragastrically to DSS-induced UC mice for 14 days. The Disease Activity Index (DAI) and histological staining were measured to detect histopathological changes in colon. The levels of IL-1β, IL-6, and TNF-α in serum were measured by ELISA. The protein and mRNA expression of HMGB1/NLRP3/Caspase-1 in the colon were detected by immunohistochemistry, western Blotting or qRT-PCR.

Results

MAT improved the histological pathological changes of UC mice, as assessed by DAI, colonic length, and colonic mucosal injury. MAT also reduced colonic inflammatory damage by reducing the serum IL-1β, IL-6, and TNF-α content and decreasing the expression of HMGB1, NLRP3, Caspase-1, and IL-1β and proteins and mRNA in the colon.

Conclusion

MAT could significantly alleviate DSS-induced UC symptoms by reducing the expressions of pro-inflammatory cytokines, such as IL-1β, TNF-α, and IL-6, the mechanism of which is related to the inhibition of HMGB1/NLRP3/Caspase-1 signaling pathway.

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2024-02-12
2025-03-29

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/content/journals/cchts/10.2174/0113862073292384240209095838
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Matrine: A Promising Treatment for Ulcerative Colitis by Targeting the HMGB1/NLRP3/Caspase-1 Pathway
Comb Chem High Throughput Screen 28, 654 (2025); https://doi.org/10.2174/0113862073292384240209095838
/content/journals/cchts/10.2174/0113862073292384240209095838
/content/journals/cchts/10.2174/0113862073292384240209095838
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  • Article Type:     Research Article
Keyword(s): colon; HMGB1/NLRP3/Caspase-1 signaling pathway; inflammation; Matrine (MAT); serum; ulcerative colitis (UC)

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