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Volume 28, Issue 1
  • ISSN: 1386-2073
  • E-ISSN: 1875-5402

Abstract

Background

Taurine upregulated gene 1 (TUG1) has been identified on long non-coding RNA (lncRNA); however, its function in myocardial cells following ischemia/reperfusion (I/R) injury has not been explored. This study aimed to investigate the role of LncTUG1 in I/R injury by focusing on its relationship with autophagy induction by regulating miR-34a-5p expression.

Methods

We established a myocardial I/R model and H9C2 hypoxia-ischemic and reoxygenation (HI/R) conditions to induce I/R injury. TTC, Western blot, CCK-8 assay, quantitative reverse transcription PCR, flow cytometry, and confocal microscopy were used to assess the size of myocardial infarct, level of some apoptotic-related and autophagy-associated proteins, cell viability, the level of LncRNA TUG1, apoptosis, and autophagy, respectively.

Results

The results revealed that a TUG1 knockdown protected against I/R-induced myocardial injury by decreasing the impairment in cardiac function. LncRNA TUG1 expression was increased in a myocardial I/R model and HI/R in H9C2 cells. Moreover, inhibition of LncTUG1 enhanced H9C2 cell viability and protected the cells from HI/R-induced apoptosis. Silencing LncRNA TUG1 promoted HI/R-induced autophagy. Furthermore, TUG1 siRNA upregulated the level of miR-34a-5p compared to the HI/R group. The protective effect of LncRNA TUG1 inhibition on H9C2 cells following HI/R was eliminated by blocking autophagy with an miR-34a-5p inhibitor.

Conclusion

These findings indicated that inhibiting TUG1 may reduce the extent of myocardial I/R injury by regulating miR-34a-5p. Taken together, these results suggest that LncRNA TUG1 may represent a novel therapeutic target for myocardial I/R injury.

© 2025 Bentham Science Publishers
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2024-01-30
2025-01-19

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Inhibition of Taurine-upregulated Gene 1 Upregulates MiR-34a-5p to Protect against Myocardial Ischemia/Reperfusion via Autophagy Regulation
Comb Chem High Throughput Screen 28, 110 (2025); https://doi.org/10.2174/0113862073267559231106074309
/content/journals/cchts/10.2174/0113862073267559231106074309
/content/journals/cchts/10.2174/0113862073267559231106074309
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  • Article Type:     Research Article
Keyword(s): autophagy; ischemia/reperfusion injury; LncTUG1; miR-34a-5p; myocardial; nucleotides (nt)

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