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image of Evaluation of Antioxidant Properties and Antihyperlipidemic Activity of Phytochemicals in Luffa acutangula Leaves

Abstract

Background

Hyperlipidaemia, characterized by elevated triglyceride and cholesterol levels in the blood, is linked to premature aging and degenerative diseases, often driven by free radicals. Antioxidants can help address these issues by neutralizing free radicals. leaves are known for their antioxidant properties due to their content of flavonoids and phenolic compounds.

Objective

This study aimed to extract, fractionate, and analyze the phytochemical composition of leaves and to evaluate their antioxidant and antihyperlipidemic activities across different fractions: ethyl acetate, chloroform, hexane, and methanol.

Methods

The extraction was performed using 96% methanol and liquid-liquid fractionation with solvents n-hexane, ethyl acetate, chloroform, and methanol. Phytochemical screening and antioxidant activity assessments were conducted on the methanolic extract, with LC-MS/MS analysis revealing various phytoconstituents. Antioxidant activity was measured using standard methods, along with enzyme inhibition assays.

Results

Methanol extraction yielded 19.73% of the extract, with the methanol fraction providing the highest dried extract (41.64%), followed by the ethyl acetate fraction at 14.09%. The methanolic extract contained flavonoids, phenolic compounds, and other phytoconstituents. The ethyl acetate fraction showed the lowest IC value (22.04, 115.2, and 69.74) for DPPH, ABTS, and FRAP, respectively, demonstrating the strongest antioxidant potential. The ethyl acetate fraction displayed maximum antihyperlipidemic potential (IC-17.85 for HMG-CoA reductase). Each fraction exhibited varying yields and secondary metabolites, with the ethyl acetate fraction showing the most significant antioxidant and antihyperlipidemic effects.

Conclusion

This study provided comprehensive information regarding the extraction, fractionation, and phytochemical composition of leaves, noting that the ethyl acetate fraction possesses significant antioxidant and antihyperlipidemic potential.

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2024-12-17
2025-04-23

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Evaluation of Antioxidant Properties and Antihyperlipidemic Activity of Phytochemicals in Luffa acutangula Leaves
Bentham Science Publishers ; https://doi.org/10.2174/0122127968351878241204103648
/content/journals/ccb/10.2174/0122127968351878241204103648
/content/journals/ccb/10.2174/0122127968351878241204103648
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  • Article Type:     Research Article
Keywords: antioxidants ; obesity ; Luffa acutangula ; extraction ; fraction ; antihyperlipidemic

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