Formulation of Thiamine-Laden Chitosan Nanosphere for Nutraceutical Application

Introduction: Thiamine, often known as vitamin B1, is a water-soluble vitamin that can be added to food or purchased as a supplement. It plays an important role in many cellular processes and is crucial for overall human health. The synthesis and characterization of Thiamineencapsulated chitosan nanospheres for sustained release and improved bioavailability is the major objective of this study. Materials and Methods: The chitosan nanospheres were prepared using sodium tripolyphosphate (TPP) as cross-linking agent. Results: The ionic interactions between chitosan and TPP produced small-sized stable nanospheres for the incorporation of Thiamine. The conjugate was characterized through a UV spectrophotometer and Fourier Transform Infrared spectroscopy (FTIR). The size and morphology were determined using Zetasizer and Scanning Electron Microscopy (SEM). An average size of 504 nm size was obtained for the nanospheres. The in vitro release studies were performed for 30 days to assess the sustained release of thiamine from nanoencapsulates. Cellular uptake of the thiamine-encapsulated chitosan nanoparticles was studied in the human cervical cancer cell lines (HeLa) and mus-musculus 3T3 L1 cell lines. Conclusion: This study is an early design and development of a technology for encapsulating water-soluble vitamins in biodegradable polymers, which can be examined for the vitamin's long-term bioavailability.