Skip to content
2000
  1. Home
  2. A-Z Publications
  3. Current Bioactive Compounds
  4. Fast Track Articles
  5. Fast Track Article
image of Cucurbitacin-E; Curvularia lunata Secondary Metabolite as a BRCA 1 and 2 Regulator in Mice Associated Breast Cancer

Abstract

Background

Cucurbitacin-induced apoptosis and inhibition of cell growth can render several cancers ineffective. The microbial transformation of cucurbitacin-E-glucoside to cucurbitacin-E was carried out by (NRRL 2178). Moreover, anticancer activity against the MCF-7 cell line as well as in vivo anticancer activity against dimethylbenz (a) anthracene (DMBA)-induced breast cancer in mice using cucurbitacin-E was evaluated.

Methods

The cucurbitacin-E-glucoside was biotransformed by to cucurbitacin-E, and the isolated compound was tested in vitro against the MCF-7 cell line, and its IC was calculated. LD50 of cucurbitacin-E was estimated in mice, and its protective activity against DMBA-induced cancer in mice was studied. Breast cancer induction was done by a single-dose subcutaneous administration of DMBA (50 mg). Plasma ALT, AST, ALP, and LDH, as well as GSH, SOD, GPx, MDA, TNF-α, IL-6, and tumor suppressor P53 assays, were used to assess cucurbitacin-E's capacity to protect the liver and breast against DMBA-induced toxicity. Moreover, by assessing the gene expression of tumor suppressor genes (BRCA 1 and 2) and conducting histopathological analysis, the suppressive effect of cucurbitacin-E was examined.

Results

The IC value of cucurbitacin-E against MCF-7 cell lines equals 72.15 ± 0.64 µg/ml. LD of cucurbitacin-E given orally in adult mice is equal to 1200 mg/kg b.w. The levels of plasma ALT, AST, ALP, and LDH were decreased significantly in DMBA-treated mice when administered with cucurbitacin-E at 1/50 LD (24 mg/kg/b.w.) and 1/20 LD (60 mg/kg/b.w.). In breast tissue, the levels of GSH, SOD, GPx, and P53 were significantly increased, as were decreased levels of TNF-α, IL-6, P53, and MDA. Conversely, there was a downregulation in the mRNA expression levels of BRCA1 and BRCA2. The histopathological analysis revealed that cucurbitacin-E management improved the tissue architecture of breast tumors.

Conclusions

These findings demonstrate the ability of cucurbitacin-E to inhibit cancer cells in the rat breast by controlling oxidative stress and inflammatory biomarkers, as well as downregulating the mRNA expression levels of BRCA1 and BRCA2.

© 2024 Bentham Science Publishers
Loading

Article metrics loading...

/content/journals/cbc/10.2174/0115734072319208240928142003
2024-10-09
2024-11-15

  • From This Site

    /content/journals/cbc/10.2174/0115734072319208240928142003
    dcterms_title,dcterms_subject,pub_keyword
    -contentType:Contributor -contentType:Concept -contentType:Institution
    10
    5
Loading full text...

Full text loading...

References

  1. WHO, Breast cancer. 2023 Available from:https://www.who.int/news-room/fact-sheets/detail/breast-cancer(accessed on 31-8-2024)
  2. Yin S.Y. Wei W.C. Jian F.Y. Yang N.S. Therapeutic applications of herbal medicines for cancer patients. Evid. Based Complement. Alternat. Med. 2013 2013 1 15 10.1155/2013/302426 23956768
    [Google Scholar]
  3. Shirode A.B. Bharali D.J. Nallanthighal S. Coon J.K. Mousa S.A. Reliene R. Nanoencapsulation of pomegranate bioactive compounds for breast cancer chemoprevention. Int. J. Nanomedicine 2015 10 475 484 25624761
    [Google Scholar]
  4. El Gizawy H.A.E.H. Hussein M.A. Abdel-Sattar E. Biological activities, isolated compounds and HPLC profile of Verbascum nubicum. Pharm. Biol. 2019 57 1 485 497 10.1080/13880209.2019.1643378 31401911
    [Google Scholar]
  5. Hussein M.A. Prophylactic effect of resveratrol against ethinylestradiol-induced liver cholestasis. J. Med. Food 2013 16 3 246 254 10.1089/jmf.2012.0183 23305807
    [Google Scholar]
  6. Hussein M.A. Ismail N.E.M. Mohamed A.H. Borik R.M. Ali A.A. Mosaad Y.O. Plasma phospholipids: A promising simple biochemical parameter to evaluate COVID-19 infection severity. Bioinform. Biol. Insights 2021 15 10.1177/11779322211055891 34840499
    [Google Scholar]
  7. Shehata M.R. Mohamed M.M.A. Shoukry M.M. Hussein M.A. Hussein F.M. Hussein F.M. Synthesis, characterization, equilibria and biological activity of dimethyltin(IV) complex with 1,4-piperazine. J. Coord. Chem. 2015 68 6 1101 1114 10.1080/00958972.2015.1007962
    [Google Scholar]
  8. Mohammed Abdalla H. Jr Soad Mohamed A.G. In vivo Hepato-protective Properties of Purslane Extracts on Paracetamol-Induced Liver Damage. Malays. J. Nutr. 2010 16 1 161 170 22691863
    [Google Scholar]
  9. Mohamad E.A. Mohamed Z.N. Hussein M.A. Elneklawi M.S. GANE can improve lung fibrosis by reducing inflammation via promoting p38MAPK/TGF-β1/NF-κB signaling pathway downregulation. ACS Omega 2022 7 3 3109 3120 10.1021/acsomega.1c06591 35097306
    [Google Scholar]
  10. El Gizawy H.A. Abo-Salem H.M. Ali A.A. Hussein M.A. Phenolic profiling and therapeutic potential of certain isolated compounds from Parkia roxburghii against AChE activity as well as GABA A α5, GSK-3β, and p38α MAP-kinase genes. ACS Omega 2021 6 31 20492 20511 10.1021/acsomega.1c02340 34395996
    [Google Scholar]
  11. Gobba N.A.E.K. Hussein Ali A. El Sharawy D.E. Hussein M.A. The potential hazardous effect of exposure to iron dust in Egyptian smoking and nonsmoking welders. Arch. Environ. Occup. Health 2018 73 3 189 202 10.1080/19338244.2017.1314930 28375782
    [Google Scholar]
  12. Teoh P.L. Liau M. Cheong B.E. Phylanodiflora L. Extracts ınduce apoptosis and cell cycle arrest in human breast cancer cell line, MCF-7. Nutr. Cancer 2019 71 4 668 675 10.1080/01635581.2018.1559942 30663402
    [Google Scholar]
  13. Li X. Qu L. Dong Y. Han L. Liu E. Fang S. Zhang Y. Wang T. A review of recent research progress on the astragalus genus. Molecules 2014 19 11 18850 18880 10.3390/molecules191118850 25407722
    [Google Scholar]
  14. Chen J.C. Chiu M.H. Nie R.L. Cordell G.A. Qiu S.X. Cucurbitacins and cucurbitane glycosides: structures and biological activities. Nat. Prod. Rep. 2005 22 3 386 399 10.1039/b418841c 16010347
    [Google Scholar]
  15. Lee D.H. Iwanski G.B. Thoennissen N.H. Cucurbitacin: ancient compound shedding new light on cancer treatment. ScientificWorldJournal 2010 10 413 418 10.1100/tsw.2010.44 20209387
    [Google Scholar]
  16. Tannin-Spitz T. Grossman S. Dovrat S. Gottlieb H.E. Bergman M. Growth inhibitory activity of cucurbitacin glucosides isolated from Citrullus colocynthis on human breast cancer cells. Biochem. Pharmacol. 2007 73 1 56 67 10.1016/j.bcp.2006.09.012 17049494
    [Google Scholar]
  17. Wakimoto N. Yin D. O’Kelly J. Haritunians T. Karlan B. Said J. Xing H. Koeffler H.P. Cucurbitacin B has a potent antiproliferative effect on breast cancer cells in vitro and in vivo. Cancer Sci. 2008 99 9 1793 1797 10.1111/j.1349‑7006.2008.00899.x 18627377
    [Google Scholar]
  18. Yang L. Wu S. Zhang Q. Liu F. Wu P. 23,24-Dihydrocucurbitacin B induces G2/M cell-cycle arrest and mitochondria-dependent apoptosis in human breast cancer cells (Bcap37). Cancer Lett. 2007 256 2 267 278 10.1016/j.canlet.2007.06.018 17681423
    [Google Scholar]
  19. Choudhary M.I. Zafar S. Khan N.T. Ahmad S. Noreen S. Marasini B.P. Al-Khedhairy A.A. Atta-ur-Rahman Biotransformation of dehydroepiandrosterone with Macrophomina phaseolina and β-glucuronidase inhibitory activity of transformed products. J. Enzyme Inhib. Med. Chem. 2012 27 3 348 355 10.3109/14756366.2011.590804 21774747
    [Google Scholar]
  20. Devkota K.P. Choudhary M.I. Nawaz S.A. Lannang A.M. Lenta B.N. Fokou P.A. Sewald N. Microbial transformation of the steroidal alkaloid dictyophlebine by Rhizopus stolonifer. Chem. Pharm. Bull. (Tokyo) 2007 55 4 682 684 10.1248/cpb.55.682 17409573
    [Google Scholar]
  21. Tong W.Y. Dong X. Microbial biotransformation: recent developments on steroid drugs. Recent Pat. Biotechnol. 2009 3 2 141 153 10.2174/187220809788700157 19519569
    [Google Scholar]
  22. Henry N.L. Azzouz F. Desta Z. Li L. Nguyen A.T. Lemler S. Hayden J. Tarpinian K. Yakim E. Flockhart D.A. Stearns V. Hayes D.F. Storniolo A.M. Predictors of aromatase inhibitor discontinuation as a result of treatment-emergent symptoms in early-stage breast cancer. J. Clin. Oncol. 2012 30 9 936 942 10.1200/JCO.2011.38.0261 22331951
    [Google Scholar]
  23. Wang S. Lu Z. Lang B. Wang X. Li Y. Chen J. Curvularia lunata and Curvularia Leaf Spot of Maize in China. ACS Omega 2022 7 51 47462 47470 10.1021/acsomega.2c03013 36591195
    [Google Scholar]
  24. Hou J.M. Ma B.C. Zuo Y.H. Guo L.L. Gao S.G. Wang Y.Y. Liu T. Rapid and sensitive detection of Curvularia lunata associated with maize leaf spot based on its Clg2p gene using semi-nested PCR. Lett. Appl. Microbiol. 2013 56 4 245 250 10.1111/lam.12040 23278833
    [Google Scholar]
  25. Lu Y. Song Y. Xue Z. Multiplex polymerase chain reaction detection of Curvularia lunata, Bipolaris maydis, and Aureobasidium zeae in infected maize leaf tissues. J. Basic Microbiol. 2019 59 9 862 866 10.1002/jobm.201900185 31330054
    [Google Scholar]
  26. Patil B.B. Chincholkar S. Influence of substrate preparation method on rifamycin oxidase activity of C. lunata. Indian J. Exp. Biol. 1997 35 917 919
    [Google Scholar]
  27. He S. Ou R. Wang W. Ji L. Gao H. Zhu Y. Liu X. Zheng H. Liu Z. Wu P. Lu L. Camptosorus sibiricus rupr aqueous extract prevents lung tumorigenesis via dual effects against ROS and DNA damage. J. Ethnopharmacol. 2018 220 44 56 10.1016/j.jep.2017.12.021 29258855
    [Google Scholar]
  28. Yuyama K.T. Fortkamp D. Abraham W.R. Eremophilane-type sesquiterpenes from fungi and their medicinal potential. Biol. Chem. 2017 399 1 13 28 10.1515/hsz‑2017‑0171 28822220
    [Google Scholar]
  29. van de Loosdrecht A.A. Beelen R.H. Ossenkoppele G.J. Broekhoven M.G. A tetrazolium based colorimetric MTT assay to quantitate human monocyte mediated cytotoxicity against leukemic cells from cell lines and patients with acute myeloid leukemia. J. Immunol. Methods. 1994 174 1-2 311 320 10.1016/0022‑1759(94)90034‑5
    [Google Scholar]
  30. Abal P. Louzao M. Antelo A. Alvarez M. Cagide E. Vilariño N. Vieytes M. Botana L. Acute Oral Toxicity of Tetrodotoxin in Mice: Determination of Lethal Dose 50 (LD50) and No Observed Adverse Effect Level (NOAEL). Toxins (Basel) 2017 9 3 75 10.3390/toxins9030075 28245573
    [Google Scholar]
  31. Ross D.S. Steele G. Jr Rodrick M.L. Milford E. Bleday R.S. Lahey S.J. Deasy J.M. Rayner A.A. Wilson R.E. The effect of 1,2‐dimethylhydrazine (DMH) carcinogenesis on peripheral T cell subsets in the wistar furth rat. J. Surg. Oncol. 1984 26 4 238 244 10.1002/jso.2930260408 6236335
    [Google Scholar]
  32. Kim C.S. Choi S.J. Park C.Y. Li C. Choi J.S. Effects of silybinin on the pharmacokinetics of tamoxifen and its active metabolite, 4-hydroxytamoxifen in rats. Anticancer Res. 2010 30 1 79 85 20150620
    [Google Scholar]
  33. Reitman S. Frankel S. A colorimetric method for the determination of plasma oxaloacetic acid and glutamic pyruvic transaminases. Am. J. Clin. Pathol. 1957 28 56 63 10.1093/ajcp/28.1.56 13458125
    [Google Scholar]
  34. Kind P.R.N. King E.J. Estimation of plasma phosphatase by determination of hydrolysed phenol with amino-antipyrine. J. Clin. Pathol. 1954 7 4 322 326 10.1136/jcp.7.4.322 13286357
    [Google Scholar]
  35. Buhl S.N. Jackson K.Y. Optimal conditions, and comparison of lactate dehydrogenase catalysis of the lactate to pyruvate to lactate reactions in human plasma at 25, 30 and 37 0C. Clin. Chem. 1978 2415 828 10.1093/clinchem/24.5.828 25724
    [Google Scholar]
  36. Uchiyama M. Mihara M. Determination of malonaldehyde precursor in tissues by thiobarbituric acid test. Anal. Biochem. 1978 86 1 271 278 10.1016/0003‑2697(78)90342‑1 655387
    [Google Scholar]
  37. Chanarin I. Textbook of Laboratory Hematology: An Account of Laboratory Techniques New York Churchill Livingstone 1989
    [Google Scholar]
  38. Marklund S. Marklund G. Involvement of the superoxide anion radical in the autoxidation of pyrogallol and a convenient assay for superoxide dismutase. Eur. J. Biochem. 1974 47 3 469 474 10.1111/j.1432‑1033.1974.tb03714.x 4215654
    [Google Scholar]
  39. Paglia D.E. Valentine W.N. Studies on the quantitative and qualitative characterization of erythrocyte glutathione peroxidase. J. Lab. Clin. Med. 1967 70 1 158 169 6066618
    [Google Scholar]
  40. Alturkistani H.A. Tashkandi F.M. Mohammedsaleh Z.M. Histological Stains: A Literature Review and Case Study. Glob. J. Health Sci. 2015 8 3 72 79 10.5539/gjhs.v8n3p72 26493433
    [Google Scholar]
  41. Petronelli A. Pannitteri G. Testa U. Triterpenoids as new promising anticancer drugs. Anticancer Drugs 2009 20 10 880 892 10.1097/CAD.0b013e328330fd90 19745720
    [Google Scholar]
  42. Garcia-Granados A. Martinez A. Parra A. Rivas F. Manoyl-oxide biotransformations with filamentous fungi. Curr. Org. Chem. 2007 11 8 679 692 10.2174/138527207780598774
    [Google Scholar]
  43. Arantes S. Hanson J. The biotransformation of sesquiterpenoids by Mucor plumbeus. Curr. Org. Chem. 2007 11 8 657 663 10.2174/138527207780598747
    [Google Scholar]
  44. Parra A. Rivas F. Garcia-Granados A. Martinez A. Microbial transformation of triterpenoids. Mini Rev. Org. Chem. 2009 6 4 307 320 10.2174/157019309789371569
    [Google Scholar]
  45. Changtam C. Sukcharoen O. Yingyongnarongkul B. Chimnoi N. Suksamrarn A. Functional group-mediated biotransformation by Curvularia lunata NRRL 2178: synthesis of 3-dehydro-2-deoxy-ecdysteroids from the 3-hydroxy-2-mesyloxy analogues. Tetrahedron 2008 64 11 2626 2633 10.1016/j.tet.2008.01.033
    [Google Scholar]
  46. Tang Y. Li W. Cao J. Li W. Zhao Y. Bioassay-guided isolation and identification of cytotoxic compounds from Bolbostemma paniculatum. J. Ethnopharmacol. 2015 169 18 23 10.1016/j.jep.2015.04.003 25882313
    [Google Scholar]
  47. Jiang H. Tan R. Jiao R. Deng X. Tan R. Herpecaudin from Herpetospermum caudigerum, a Xanthine Oxidase Inhibitor with a Novel Isoprenoid Scaffold. Planta Med. 2016 82 11/12 1122 1127 10.1055/s‑0042‑108210 27272398
    [Google Scholar]
  48. Cheng A.C. Hsu Y.C. Tsai C.C. The effects of cucurbitacin E on GADD45β‐trigger G2/M arrest and JNK‐independent pathway in brain cancer cells. J. Cell. Mol. Med. 2019 23 5 3512 3519 10.1111/jcmm.14250 30912292
    [Google Scholar]
  49. Hsu Y-C. Chen M-J. Huang T-Y. Inducement of mitosis delay by cucurbitacin E, a novel tetracyclic triterpene from climbing stem of Cucumis melo L., through GADD45γ in human brain malignant glioma (GBM) 8401 cells. Cell Death Dis. 2014 5 2 e1087 10.1038/cddis.2014.22 24577085
    [Google Scholar]
  50. Borik R.M. Hussein M.A. Synthesis, molecular docking, biological potentials and structure-activity relationship of new quinazoline and quinazoline-4-one derivatives. Asian J. Chem. 2021 33 2 423 438 10.14233/ajchem.2021.23036
    [Google Scholar]
  51. Boshra S.A. Hussein M.A. Cranberry extract as a supplemented food in treatment of oxidative stress and breast cancer induced by N-Methyl-N-Nitrosourea in female virgin rats. Int. J. Phytomed. 2016 8 217 227
    [Google Scholar]
  52. Hussein M.A. Borik R.M. A novel quinazoline-4-one derivatives as a promising cytokine inhibitor: Synthesis, molecular docking, and structure-activity relationship. Curr. Pharm. Biotechnol. 2022 23 9 1179 1203 10.2174/1389201022666210601170650 34077343
    [Google Scholar]
  53. Mostafa M.M. Amin M.M. Zakaria M.Y. Hussein M.A. Shamaa M.M. Abd El-Halim S.M. Chitosan Surface-Modified PLGA Nanoparticles Loaded with Cranberry Powder Extract as a Potential Oral Delivery Platform for Targeting Colon Cancer Cells. Pharmaceutics 2023 15 2 606 10.3390/pharmaceutics15020606 36839928
    [Google Scholar]
  54. Sonpavde G. Pond G.R. Berry W.R. de Wit R. Armstrong A.J. Eisenberger M.A. Tannock I.F. Serum alkaline phosphatase changes predict survival independent of PSA changes in men with castration-resistant prostate cancer and bone metastasis receiving chemotherapy. Urol. Oncol. 2012 30 5 607 613 10.1016/j.urolonc.2010.07.002 20888271
    [Google Scholar]
  55. Chung S.O. Kim Y.J. Park S.U. An updated review of Cucurbitacins and their biological and pharmacological activities. EXCLI J. 2015 14 562 566 10.17179/excli2015‑283 26648815
    [Google Scholar]
  56. Lu J. Ding T. Qin X. Liu M. Wang X. In vitro and in vivo evaluation of cucurbitacin E on rat hepatic CYP2C11 expression and activity using LC-MS/MS. Sci. China Life Sci. 2017 60 2 215 224 10.1007/s11427‑015‑4911‑7 26354504
    [Google Scholar]
  57. Xie H. Tuo X. Zhang F. Bowen L. Zhao W. Xu Y. Dietary cucurbitacin e reduces high-strength altitude training induced oxidative stress, inflammation and immunosuppression. Acad. Bras. Cienc. 2020 92 4 e20200012
    [Google Scholar]
  58. M Soliman S. Mosallam S. Mamdouh M.A. Hussein M.A. M Abd El-Halim S. Design and optimization of cranberry extract loaded bile salt augmented liposomes for targeting of MCP-1/STAT3/VEGF signaling pathway in DMN-intoxicated liver in rats. Drug Deliv. 2022 29 1 427 439 10.1080/10717544.2022.2032875 35098843
    [Google Scholar]
  59. Arulkumaran S. Ramprasath V.R. Shanthi P. Sachdanandam P. Alteration of DMBA-induced oxidative stress by additive action of a modified indigenous preparation—Kalpaamruthaa. Chem. Biol. Interact. 2007 167 2 99 106 10.1016/j.cbi.2007.01.013 17349985
    [Google Scholar]
  60. Hussein M.A. Borik R.M. A Novel Quinazoline-4-one Derivatives as a Promising Cytokine Inhibitors: Synthesis, Molecular Docking, and Structure-activity Relationship. Curr. Pharm. Biotechnol. 2022 23 9 1179 1203 10.2174/1389201022666210601170650 34077343
    [Google Scholar]
  61. El-Gizawy H.A. Hussein M.A. Fatty acids profile, nutritional values, anti-diabetic and antioxidant activity of the fixed oil of malvaparviflora growing in Egypt. Int. J. Phytomed. 2015 7 219 230
    [Google Scholar]
  62. Ahn J. Xia T. Konno H. Konno K. Ruiz P. Barber G.N. Inflammation-driven carcinogenesis is mediated through STING. Nat. Commun. 2014 5 1 5166 10.1038/ncomms6166 25300616
    [Google Scholar]
  63. Mosaad Y.O. Hussein M.A. Ateyya H. Mohamed A.H. Ali A.A. Ramadan Youssuf A. Wink M. El-Kholy A.A. Vanin 1 Gene Role in Modulation of iNOS/MCP-1/TGF-β1 Signaling Pathway in Obese Diabetic Patients. J. Inflamm. Res. 2022 15 6745 6759 10.2147/JIR.S386506 36540060
    [Google Scholar]
  64. Zhong H. Huang Y. Deng X. Liu M. Luo W. Cucurbitacin B. Cucurbitacin B supplementation reduces inflammatory responses and alveolar bone loss via regulating MPO, COX-2 and RANK/RANKL/OPG signals in a rodent model of ligature-induced periodontitis. J. King Saud Univ. Sci. 2020 32 3 1889 1895 10.1016/j.jksus.2020.01.028
    [Google Scholar]
  65. Song H. Wang Y. Li L. Sui H. Wang P. Wang F. Cucurbitacin E. Cucurbitacin E Inhibits Proliferation and Migration of Intestinal Epithelial Cells via Activating Cofilin. Front. Physiol. 2018 9 1090 10.3389/fphys.2018.01090 30131725
    [Google Scholar]
  66. De S. Sarkar B. Jadhav G.R. Ramasamy S.K. Banerjee S. Moorthy A. Paira P. K A.K.S. Experimental and Theoretical Study on the Biomolecular Interaction of Novel Acenaphtho Quinoxaline and Dipyridophenazine Analogues. ChemistrySelect 2018 3 38 10593 10602 10.1002/slct.201801448
    [Google Scholar]
  67. Kuo Y.C. Yen M.H. De S. Rajesh R. Tai C.K. Optimized lipopolymers with curcumin to enhance AZD5582 and GDC0152 activity and downregulate inhibitors of apoptosis proteins in glioblastoma multiforme. Biomaterials Advances 2023 154 213639 10.1016/j.bioadv.2023.213639 37793310
    [Google Scholar]
  68. Kuo Y.C. Lin S.Y. De S. Rajesh R. Regeneration of Pancreatic Cells Using Optimized Nanoparticles and l -Glutamic Acid–Gelatin Scaffolds with Controlled Topography and Grafted Activin A/BMP4. ACS Biomater. Sci. Eng. 2023 9 11 6208 6224 10.1021/acsbiomaterials.3c00791 37882705
    [Google Scholar]
  69. De S. Ashok Kumar S.K. Development of highly potent Arene-Ru (II)-ninhydrin complexes for inhibition of cancer cell growth. Inorg. Chim. Acta 2020 508 119641 10.1016/j.ica.2020.119641
    [Google Scholar]
/content/journals/cbc/10.2174/0115734072319208240928142003
Loading
Cucurbitacin-E; Curvularia lunata Secondary Metabolite as a BRCA 1 and 2 Regulator in Mice Associated Breast Cancer
Bentham Science Publishers ; https://doi.org/10.2174/0115734072319208240928142003
/content/journals/cbc/10.2174/0115734072319208240928142003
/content/journals/cbc/10.2174/0115734072319208240928142003
Loading

Data & Media loading...


  • Article Type:     Research Article
Keywords: Curvularia lunata ; Cucurbitacin-E ; MCF-7 ; DMBA ; P53 ; BRCA1 and BRCA2

Most Cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error
Please enter a valid_number test