Current Analytical Chemistry - Volume 3, Issue 2, 2007

Most current methods for determining protein carbonyl content are based on the reaction of carbonyl groups with 2,4-dinitrophenylhydrazine (DNPH) to form 2,4-dinitrophenylhydrazone derivatives, which can then be detected and quantitated spectrophotometrically or immunochemically. In this article, we evaluate different quantitative and semi-quantitative techniques for measuring protein carbonylation. We assessed the Read More

The advent of the “omics” revolution has reawakened an interest in the mass spectrometric analysis of bile acids, particularly in the related fields of lipidomics and metabolomics. This is due to the presence of bile acids in body fluids and their potential to act as biomarkers. Bile acids and bile alcohols are formed from cholesterol in the liver. Bile acids are excreted from the liver into the small intestine via the bile duct conjugat Read More

The objective of this paper is to contribute to the methodology available for extracting and analyzing signal content from protein mass spectrometry data. Data from Matrix-Assisted Laser Desorption Ionization Time-of-Flight (MALDI-TOF) or Surface-Enhanced Laser Desorption and Ionization Time-Of-Flight (SELDI-TOF) spectra require considerable signal pre-processing such as noise removal and baseline level error Read More

Virgin olive oil, obtained from Olea europaea L. (Oleaceae) fruits, is an important ingredient in the Mediterranean diet. There is now overwhelming scientific evidence that it has health benefits; that include reduction of risk factors for coronary heart disease and the prevention of several pathologies, including some types of cancer. Olive oil appears to be an example of a functional food with a high content of volatiles that Read More

Calixarenes, following cyclodextrines and crown ethers, are the third generation of supramolecules used in HPLC as stationary phases. They consist of phenol units linked via methylene bridges and can also form inclusion complexes like the other host supramolecules. The resulting interactions influence the retention factors and improve the selectivity of the solutes. Additionally, modification of the calixarenes, for instance by va Read More