Current Analytical Chemistry - Volume 3, Issue 1, 2007

With over 500 members catalyzing an estimated 100,000 phosphorylation reactions, the kinases are among the largest and most complex families of enzymes. As phosphorylation represents the pivotal mechanism for regulation of biological processes, they are also one of the most biologically significant. Development of analytical techniques for the characterization of phosphorylation events, in particular from a global vantage Read More

Sensitive yet selective techniques for characterizing protein unfolding are important for assessing the conformational stability of wild-type and recombinant protein. This review presents a summary of recent developments in protein unfolding by capillary electrophoresis (CE). Relative to conventional spectroscopic methods, CE offers a versatile microseparation format for performing unfolding studies using small amounts Read More

Since ancient times many natural organic materials have been used in artistic and historic works as binders, adhesives, fillers and coatings. The identification of these materials is important not only for a proof of authenticity; for restorers and conservators it is essential to recognize the materials and technologies employed by artists and craftsmen. For identification and characterisation of the different natural organic bi Read More

A simple, rapid, selective and sensitive electrochemical method for the direct determination of prednisolone in spiked human serum and tablets was developed. The electrochemical oxidation and determination of prednisolone has been carried out at glasy carbon electrode (GCE) in various aquaeous solution in the pH range of 0.56-12.30 by cyclic (CV) and Osteryoung square wave voltammetry (OSWV). The best results wer Read More

Heteronuclear coherence transfer in liquid-state NMR applications has been traditionally performed using pulse-interrupted delay schemes such as INEPT-type pulse trains. So far, the alternative use of heteronuclear crosspolarization (HCP) has only been limited to a few cases involving exclusively in-phase to in-phase transfers. In this revision work a theoretical description on the effect and the characteristic anisotropic f Read More

One of the most important ecological problems is mutagenic pollution in the environment, and the determination of mutagenic compounds in environmental samples is of special interest. In the current article, a review of the liquid chromatography-mass spectrometry (LC/MS) based methods published so far for the determination of mutagens in the environment is presented. Mutagens included in this review are poly Read More

Early studies in to lipids that comprised cellular membranes noted an asymmetrical distribution of lipids between different membranes which contrasted with the contemporary concept of the plasma membrane as a homogeneous mixture of lipid in which both lipids and membrane-associated proteins exhibit unfettered mobility. The ability of lipids to form inhomogeneities capable of restricting lateral mobility has sin Read More