Current Analytical Chemistry - Volume 2, Issue 4, 2006

This review outlines the mass spectrometric methods used to identify disulfide functionality in peptides, particularly cleavages from peptide (M-H)- parent anions. A brief introduction to characteristic negative ion fragmentations of peptides is given, including (i) the α and β cleavages which provide data analogous to that provided by Y+2 and B cleavages of MH+ ions, (ii) characteristic side chain cleavages, and (iii) γ backbon Read More

In this review, a full discussion and update of the state-of-the-art of separation techniques interfaced to plasma spectrometers for speciation analysis of non-metallic elements, such as iodine, chlorine, bromine and fluorine as well as phosphorus and sulfur is presented. The plasma-based techniques covered include inductively coupled plasma mass spectrometry (ICP-MS) microwave-induced plasma optical emission spectr Read More

Nicotinamide containing cofactors are ubiquitous in biological systems. As a result, when studying nicotinamide dependent systems in vitro or in vivo, isotopically labeled nicotinamide cofactors are often used to reveal the mechanism of the biological system of interest. The reduced form of these cofactors is labile and is sensitive to light, oxygen, pH, temperature and other factors. Consequently, their synthesis, purification, a Read More

Compare with normal liquid chromatography (LC), high-speed ion chromatography (IC) has the benefits of fast analysis, fast method development, and increased mass sensitivity. These benefits start to attract more and more researchers' attention. This review covers recent developments in high-speed IC field, mainly focus on application of the inorganic anions and cations determination; the review show that high-speed IC m Read More

Lanthanide complexes have been used for fluorescent labels to the wide variety of analytical methods including high performance liquid chromatography, immunoassay, and fluorescent imaging with time-resolved fluorometric measurements. Introduction of a time-resolved fluorometric measurement has been permitted the easy distinction of a specific long-lived fluorescence signal from short-lived natural b Read More

In this paper, the desirable physical-chemical properties of ionic liquids, the method of ionic liquids used as running electrolytes or modifier in aqueous and non-aqueous capillary electrophoresis were introduced. The potential of capillary electrophoresis based on ionic liquids was discussed throughout the paper. The application status of capillary electrophoresis based on ionic liquids in the analysis of the bioactive componen Read More

The management of phosphorus (P) as a non-renewable resource is receiving increasing attention at the dawn of the new millennium. The importance of phosphate as a nutrient, the environment phosphate load in areas with intens livestock production and the depletion of the high quality phosphate resources are accentuating the need of a holistic and global phosphorus-resource management and understanding of Read More