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Volume 23, Issue 1
  • ISSN: 2211-3525
  • E-ISSN: 2211-3533

Abstract

Background

is a herbaceous climber species within the Vitaceae family, found in various tropical and subtropical regions of Africa. It has a history of traditional use in Ethiopia for treating various health issues in humans and livestock, including snake bites, rabies, and eczema. Despite its reported ethnomedicinal uses, there is a lack of sufficient scientific literature regarding the isolation and characterization of its bioactive chemical compounds.

Objective

The objective of this work was to isolate bioactive compounds from the leaves of , characterize their chemical structures using FTIR and NMR spectroscopy, and evaluate their antimicrobial activities.

Methods

Isolation was done using column chromatographic separation techniques. Spectroscopic techniques, including Fourier transform infrared and nuclear magnetic resonance, were used for functional group identification and structural elucidation. Moreover, the paper disc diffusion bioassay method was used to evaluate the antibacterial activity of the isolated bioactive compounds.

Results

Three fractions were successfully isolated using chloroform with dichloromethane and chloroform with ethyl acetate as eluting solvents and silica gel as a stationary phase. After careful interpretation of the experimental results and comparison with the literature, the isolated fractions were constituted mainly by stigmasterol (fraction ), β-sitosterol (fraction ), and trans-resveratrol (fraction ). The antibacterial activities of each isolated fraction were tested against , a gram-negative bacterium, and , a gram-positive bacterial pathogen.

Comparatively, the highest mean inhibitory value of 10.03 ± 0.13 mm was exhibited by fraction , which was followed by 6.25 ± 0.05 mm inhibition activity recorded for fraction against , while fraction did not exhibit any bacterial activity against both the strains.

Conclusion

We have successfully isolated and characterized three compounds (-) from leaves of and evaluated their antibacterial activity. A maximum mean inhibitory value of 10.03 ± 0.13 mm was exhibited by compound against .

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2024-06-03
2025-01-28

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/content/journals/aia/10.2174/0122113525306978240520053843
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Isolation, Characterization and Antibacterial Activity Study of Bioactive Compounds from Leaves of Cyphostemma cyphopetalum
Anti Infect. Agents 23, E030624230597 (2025); https://doi.org/10.2174/0122113525306978240520053843
/content/journals/aia/10.2174/0122113525306978240520053843
/content/journals/aia/10.2174/0122113525306978240520053843
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  • Article Type:     Research Article
Keyword(s): antibacterial activity; Cyphostemma cyphopetalum; Ralstonia solanacearum; stigmasterol; trans-resveratrol; β-sitosterol

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