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Volume 23, Issue 1
  • ISSN: 2211-3525
  • E-ISSN: 2211-3533

Abstract

Background

The use of commercial tinctures for the design of a formulation with improved anti-microbial activity in a polyherbal lozenge dosage form has not been described.

Objective

This study aimed to develop and evaluate the antimicrobial activity of a novel polyherbal lozenge formulation containing Ashwagandha, Neem, and Tulsi tinctures in a single-dose administration. According to previous studies, each of these herbs could be used in herbal medicine to provide relief from infection owing to their antimicrobial activity, besides other properties, such as anticancer, antidiabetic, .

Methods

The lozenges were prepared using the molding method using three concentrations (1, 3, and 5% w/w) of commercialized herbal tinctures. Then, they were studied using different assays, including those designed to evaluate physical properties, stability, and antimicrobial activity.

Results

B4, containing 5% w/w of each tincture, showed the highest antimicrobial activity compared with the other batches. This batch exhibited the highest value of bacterial inhibition (17.1 ± 0.07 mm), according to the agar well diffusion method, including as a test microorganism. Hence, B4 was chosen for additional analyses, including physical properties and stability tests. The results followed Indian Pharmacopeia standards and ICH guidelines, respectively. The formulation was stable after 2 weeks, and no significant changes were observed in its physical properties or antimicrobial activity.

Conclusion

This study shows that polyherbal lozenges have anti-microbial activity, with a disintegration period of 3.86 ± 0.07 minutes in the B4 batch.

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2024-07-05
2025-03-07

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Evaluation of Anti-microbial Activity of Novel Polyherbal Lozenges for the Management of Infection
Anti Infect. Agents 23, E050724231678 (2025); https://doi.org/10.2174/0122113525302223240606045531
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  • Article Type:     Research Article
Keyword(s): anti-microbial activity; chemical constituents; evaluation tests; Polyherbal lozenges; soft lozenges formulation; stability studies; well diffusion method

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